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microbiology capsule stain mycobacterium ziehl-neelsen smear preparation bacterial structure acid-fast staining differential staining india ink congo red nigrosine methylene blue crystal violet chromophore gram stain staining bacterial staining exosporium clostridium perfringens clostridium botulinum clostridium tetani clostridium bacillus endospore sporulation and germination medical bacteria bacteriology germination in bacteria sporulation