Gold ampule ANTIAGING

Anti-ageing Stem Cells Therapy -
Golden Vial for Rejuvenation
PROFESSOR OLEKSANDR
KUKHARCHUK
21 April, 2015

What is aging?
Theory of aging before the discovery of stem cells
was more than 300. All seems to be erroneous.
Scientific descriptions of mechanism of aging were
similar to history of the blind and the elephant… One
day an elephant was brought to a city. Six blind
people decided to know – what is elephant? – And
they continued to study. Four groped the feet of
elephant, one – trunk, and the last one – tail. Later
they said: “Elephant – it is four big thick trees”, - said
those who groped the legs. “No! This is big thick
snake”, - said by who groped the trunk. “No”, - said
the one who groped the tail, “Elephant – it is a small
thin lizard”.

.Today we exactly know that aging is related due to decrease of stem
cells in the organism of humans.
Cells of our body constantly renews, for example:
Skin – 3 days;
Stomach – 5 days;
Intestine – 7 days.
How this renewal does take place? Very simple: stem cells divide and
give rise to new cells in the organism. In all adult tissues and organs
contains stem cells without any exception. The division of cells provides
renewal of organ and tissue which gives their youth and youthfulness of
the whole organism of body. But with age, number of stem cells
progressively decreases. Cells of the tissues stop to renew and ages. And
together with cells human also ages.
So: Aging – it is decrease of stem cells reserve in organs and tissues of
the human.
CONCLUSION: For true rejuvenation need to increase stem cells reserve
in human organs and tissues.

.What are aging diseases?
Experienced doctors say that until now no one has died of
aging. All die of diseases. What are these diseases that
people die in aging? Cancer, myocardial infarction, stroke,
diabetes mellitus, Alzheimer’s disease, Parkinson,
osteoporosis, arthrosclerosis, hypertension,
immunodeficiency – this is not complete list that are named
as DISEASE OF AGING.
Why particularly these diseases kill us in aging? Answer is
very simple – they are caused by the same reason of aging:
due to decrease of stem cells reserve in the tissues of
human organism.

.Well known fact that with age the immune system weakens.
This is related with that cells of the immune system does not
renew. They age and lose their power. Such weak cells
already cannot destroy the constantly arising cancer cells
and develops carcinoma in humans. In the early stages 40-50
of yrs aging the renewal of immune system cells is already
disturbed. As the aging changes to immune cells appear,
they start to attack and destroy their own cells of organs and
tissues instead of protecting the organism. In this manner
autoimmune disease develops – diabetes mellitus, liver
cirrhosis, glomerulonephritis, arthrosclerosis, and multiple
sclerosis. Deep aging of immune system leads to
development of immune-deficiencies and aging humans die
of infectious diseases – such as inflammation of lungs,
sepsis, meningitis.

.Cells of vascular innermost membrane (intima) –
endotheliocytes renew rapidly – once in 7 days. If reserve of
stem cells forming new endotheliocytes reduces with age
then the renewal of vascular innermost membrane (intima)
does not occur. As they age covers with atherosclerotic
plague blood flow through vessels gets disturbed and people
die of stroke, myocardial infarction, thrombosis of
pulmonary artery.
Therefore: reason of aging diseases is as aging itself the
decreases of stem cells reserve in organs and human
tissues.
CONCLUSION: to block the development of aging disease it
is necessary to increase reserve of stem cells in organs and
human tissue.

.What is chronological age and biological age?
Can we know how much stem cells reserve remains in our organism? In
other words can we know how faster are we aging and when will
diseases of aging arise? Direct method of calculating number of stem
cells in the organism of living human does not exist. But there are
scientific evidential based methods of evaluating intensiveness of
human aging. One among them is the method comparison between
chronological age and biological age of humans.
Chronological aging – it is number of year from the moment of your
birth till date of your life. This is your life. This – “Age according to date
of birth”.
Biological age – this measures the change over time of the biological
possibility of organism and its back up ability to the vital systems. It
allows the prognosis of human life expectancy. This is “level of age
wear” your body depends on stem cells reserve.

.Example. Research of Zagumenova O. (Russia, 2000),
showed that in men and women (age 25-50 years,
altogether 398 people), who lived for ten years in the area
of radioactive contaminated territory after Chernobyl
nuclear station incident the biological age was higher than
that of chronological by 9.2±0.5 years. This indicates that
people in radiation zone aged to 10 years older and 79% of
people were identified with aging diseases. Pay attention!
Radiation kills active stem cells! Intensity of aging sharply
increases and radiation made people premature aging to 10
years older due to the decrease in stem cells reserve.

.Objective criteria of anti-aging therapy efficacy evaluating
Aging in each person is individual and characterized by
qualitative point of view – type of aging, by quantitative
point of view – rate of aging, with the structure of aging
point of view – ratio of different system of aging rate i.e.
profile of aging.
Differentiated 4 types of aging: cardio vascular, endocrine,
neuro-psychological, metabolic, and miscellaneous.
Profile aging determined in unevenly aging by ratio of aging
rate of different organ and system. Profile aging determined
by cardiovascular system, respiratory, muscular, nervous,
immune system, vision, hearing, and biochemical
parameters of blood.

.Standard quantitative characteristics of aging are ratio of
biological and chronological age.
Methods of determining biological age are many – from
simple to very complicated, which requires doctors
consultation and expensive analysis. Fortunately currently
developed and successfully tested method of complex
evaluation of biological age, which could be used
individually even without our participation. Program of
determination of biological age you could find internet
through links:
http://xn--b1adef0ban2h.com.ua/sam-sebe-sanolog/biologicheskij-vozrast
http://faberlic-voronezh.ru/tests/bioage/?test=1

.This would take not much of time. As a result you could
compare chronological age with biological age. This could
help you to take decision on rejuvenation (anti-aging). For
example if your chronological age is 35 years, and test shows
your current biological age is 45 then you have problem this
is fast aging which needed to fight. Otherwise decrepit
appearance and complex old age diseases are awaited
ahead. At the same time any scale in which biological age
soar up over chronological age already requires rejuvenation
even if this difference is 1 year.
Can we rejoice if chronological age and biological age
coincides? Alas, no. This indicates only about that your body
is getting older in time. Aging, but is not rejuvenating. But
ideally should be on the contrary – your biological age
should be lesser than the chronological!

.If biological age is more than the chronological, this indicates that period
of functional system aging changes accumulation beginning. Objective
characteristics of aging can be converted into new quantitative and
qualitative level – the level evaluating index of age damage and deficit of
functions.
This index calculated by formula:
The frailty index is calculated as:
The number of deficits that an individual has
The total number of deficits considered
10 deficits presents in an individual = Frailty index score of 10/40=0.25
40 deficits considered in total
Measuring frailty as an index of (40) deficits
The higher the Frailty Index, the more frail the individual
List of 40 deficit functions needed to be calculated for age damage
index, mentioned in the below table:

.
Deficit Level Deficit Level Deficit Level Deficit Level Deficit Level
Eyesight 5 Ability to take care
of appearance
3 Help to take a
bath or shower
3 Help to prepare
own meals
3 Self-rated
health
3
Hearing 5 Help to walk 3 Help to use the
telephone
3 Help to do
housework
3 Troubles
prevent normal
activities
3
Help to eat 3 Help to get in and
out of bed
3 Help to travel
beyond walking
distance
3 Ability to take
medications
3 Lives alone 2
Help to dress 3 Help to go to the
bathroom
3 Help with
shopping
3 Ability to handle
own money
3 Having cough 2
Deficit Level Deficit Level Deficit Level Deficit Level Deficit Level
Feeling tired 2 Stroke or effects of
stroke
2 Ear trouble 2 Kidney trouble 2 Trouble with
feet or ankle
2
Nose stuffed up
or sneezing
2 Arthritis or
rheumatism
2 Dental problems 2 Losing control of
bladder
2 Skin problems 2
High blood
pressure
2 Parkinson’s disease 2 Chest problems 2 Losing control of
bowels
2 Fractures 2
Heart and
circulation
problems
2 Eye trouble 2 Trouble with
stomach
2 Diabetes 2 Trouble with
nerve
2
List of 40 deficit functions needed to be calculated for age damage index, mentioned in the below table:

Wide-zoom (experimented around 70000 people) study of Rockwood et al.
(2011) revealed precise exponential relation of Frailty Index to age:
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
10 20 30 40 50 60 70 80 90 100
FrailtyIndex
Age (years)
National Population Health Survey – Mean Frailty Index at each cycle in
relation to age (Rockwood et al., CMAJ 2011; E-pub April 28)

.This study proved that Frailty Index is objectively quantitative and qualitative
criteria for evaluating aging.
In experimental study of Parks et al. (2011) was proved that Frailty Index process
of aging increases both in men and women – aging does not spare anyone.
Adult Aged
Males 0.08 0.5
Females 0.09 0.39
0
0.1
0.2
0.3
0.4
0.5
0.6
FrailtyIndex
Frailty Index inMalesandFemales
Parks et al., J Gerontol Biol Sci, 2011

Experimental study Shi et al. (2011) showed that as the Frailty Index increases
probability of death sharply rises. Thus more higher the Frailty Index lesser the
life span.
0
0.2
0.4
0.6
0.8
1
1.2
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7
DeathRate
Frailty Index
Men
Women
For men & women, deficit accumulation is highly related with mortality (r>0.95); men
have a higher death rate than women (Shi et al., BMC Geriatr. 2011 Apr 20; 11:17)

.
Conclusion: Currently are two objective parameters which characterizes aging. First
– it is difference between chronological and biological age, second – Frailty Index.
How not only to appear younger but actually to become younger?
How vital physiological systems ages?
Nervous system. Skin sensitivity, vision and hearing acuity and blood supply to tissue
reduces.
Endocrine system. Male and female hormone production (testosterone, estradiol),
and insulin reduces. In period where in men and women climax changes in all level
of endocrine system:
Immune system. Reduces all types of immune protection of the body: non-specific
reactivity, cellular and humoral immunity, anti-cancer and anti-fungal immunity.
Autoimmune aggression is developed.
Cardio-vascular system. Inner membrane of artery thickens, muscle layers get
atrophied, reduces vascular wall elasticity, vein broadens, tone of heart walls
reduces, cardiac muscle power reduces
Respiratory system. Mucosa of trachea and bronchi gets atrophied, lungs elasticity
reduces, vital capacity decreases. Atrophic and fibrotic-dystrophy process of
respiratory muscles commences.

.
Digestive system. Salivary gland, mucous and muscular membrane of the
esophagus, stomach and intestines atrophies, and secretary function
reduces. Cells of pancreatic gland starts to atrophy, number of beta-cells
reduces which produces insulin. Production of pancreatic enzymes reduces
– trypsin, amylase and lipase. Mass of liver and its size reduces. Lipids
accumulates in hepatocytes, bile flow reduces. Fibrotic musculature
changes at the bottom of gall bladder and reduction of wall elasticity leads
to increase of residual volume of bile which is capable of stone formation.
Urinary system. Renal vessels gets sclerosis, renal blood supply reduces.
After 70 yrs number of functioning nephrons reduces to 50%, glomerular
filtration rate reduces. Urinary bladder wall losses its elasticity and
thickens. Atrophy occurs in the muscular layer, contractibility feature
reduces, and sphincter weakens.
Muscle-skeletal system. Most characteristics to reduce thickness of bone
tissue – osteoporosis is the reason of bone fracture arising in the senile
group of people mostly in vertebrae, radius and femoral neck. Muscle
power reduces; they become lethargic, hypo-tropic.
THESE CHANGES OCCURS IN ALL INDIVIDUALS WITHOUT ANY EXEPTIONAL
CASES.

.
What leads the aging of vital physiological systems to?
Aging of physiological system creates a base for series of disease development. Below we
mention most common out of those: arthrosclerosis and arterial hypertension, chronic
ischemic heart disease, myocardial infarction, heart failure, stroke, cancer, autoimmune
disease, pneumonia and respiratory failure, stomach ulcers and duodenal bleeding,
perforation, penetration and malignant diseases, blindness, deafness, fracture, osteomalacia,
joint diseases, renal disease, liver, diabetes mellitus, asthenia, depression, senile dementia,
Alzheimer’s disease.
Why vital physiological system ages?
Say for instance that 100% of cells of a single organ works in young healthy body whereas
with those number of cells people live till the senile equals to 25-30% and remaining 70-75%
dies or losses its function as the result of wear and diseases. However well known that after
removal of 50% of cells from vital organs (lungs, liver, kidney, and adrenal) in the younger age
the individual can still live for long. Fact is that young stem cells enough for regeneration of
damaged organs. In the process of aging stem cells reserve sharply reduces which leads to
wear of physiological systems and diseases of aging. Subsequently for true rejuvenation
requires increasing stem cells reserve in the aging body.
So, important indicator of actual rejuvenation is period of healthy life. Successful aging –
this is absence of disease and disability, saving capability to assimilation of new information
and physical activity in family and society. That is successful aging – this is active longevity.

.Program and aim of rejuvenation with stem cells
Any treatment program has its own goals. Goals of our treatment:
1. To eliminate aging changes of vital physiological system and prevent
them from aging in near 10 years after stem cells transplantation.
2. To eliminate or to stop aging disease development – to provide
patient successful aging.
3. To normalize ratio of biological and chronological age for patients
elder than 60 yrs and reduce the chronological age in comparison with
biological age for the patients who are younger than 60 yrs.
4. To restore function of immune system in the level of young organism
5. To provide patient the external rejuvenation with the help of bio-
cosmetic procedure for hair, face, neck, décolleté, and hand.
Program includes transplantation of fetal stem cells “GOLDEN VIAL” and
special complex bio-creams which provides regeneration of hair, skin
and nails.

.What is “GOLDEN VIAL”?
To restore all functional system and rejuvenation of the patient we need
to increase of organ and tissue specific stem cells reserve in the whole
body: In brain, heart, lungs, vessels, liver, kidneys, bone marrow, tissue
of immune system and endocrine system, organs of vision and hearing,
skin, muscles and bone tissue – altogether required to renew more than
240 different types of stem cells. This can be done exclusively with the
help of fetal progenitor cells transplantation. Golden vial – it is program
of transplantation of all types stem cell (without any exception!) which
is necessary for complete renewal of all functional system and
rejuvenation.
About our complex biological active creams
Unique outstanding composition of our creams contains extracts of
stem cells specific for hair follicles, face, neck and hand. Symphony
from these musical notes of our cream is to awaken your dormant
youth reserve which returns your delight in socialization, build in
confidence, blossom and beauty!

.Creams composition: fetal growth factors of skin and hair, cytokeratins
K17, involucrin, dermal Ki-67, fibronectin and chondroitin sulfate,
collagen type III, I, glycosaminoglycans, SKALP (skin derived antileuko
proteinase), triglycerides, sterol ester, glycosphingolipids, hyaluronan,
hyaluronan stimulating activity.
Properties of creams:
Stimulation of own stem cells of the skin and hair follicle of the patient
Whitening - inhibits the melanin which gives age spots and dullness to
skin
Anti-inflammatory effect - repress pimples inflammation, redness
Moisture - restores normal moisture retaining properties of skin
Cell proliferation - improves metabolism, and generates healthy skin.
Collagen (fine, reticular, less cross-linking) production - improves
collagen generation which signifies skin elasticity.
Blood circulation – improves blood circulation
Amino acids – building blocks of the cells
Antioxidant effect – restrains active enzymes that cause aging

.1. Anti-aging Face Cream 50 ml (for 1 course).
The Cream is absorbed intensively into the dermis to strengthen the
revitalize collagen and elastin for moisture retention. Tissue extracts of
skin can regulate and normalize cases of pigmentations such as melasma
and age spots, pigmentation, acne, and scar, induce skin healing, and
help overcome other skin problems. Skin looks plump with increased
hydration and radiant luminosity. Renewed sense of skin firmness is felt.
2. Anti-aging Neck Cream 50 ml (for 1 course).
Cream gives younger, firmer, smoother skin and reduces rough and
crepey skin. The cream has powerful effect of lifting, moisturizes and
refines skin of neck and the area of decollete, eliminates undesired lines,
rejuvenates skin due to the stimulation of own stem cells of the skin.
3. Anti-aging Hand Cream 50 ml (for 1 course).
Moisturizes and refines skin of hands, eliminates undesired lines,
rejuvenates skin of hands due to the stimulation of own stem cells of the
skin. Rich, protective hand cream to deeply moisturize and restore
smoothness, Leaves hands feeling soft and supple. Provides long-lasting
hydration and natural antioxidant protection.

.
4. Anti-aging Cream for Neck and Jaw Line 50 ml (for 1 course).
Intensified lifting of skin (night). Reduces the sagging jaw by firming the skin texture,
helps in obtaining pronounced jaw-line.
5. Hair Cream 100 ml (for 1 course).
Hair follicle regeneration is characterized by dramatic changes in its microanatomy
and cellular activity. During hair follicle active growth and hair production, the
activity of factors promoting proliferation, differentiation, and survival predominates,
while hair follicle regression is characterized by activation of various signaling
pathways that induce apoptosis (programmed cell death) in hair follicle cells. Instant
stop of hair fall stimulates growth of new hair due to activation at the level of scalp
hair follicles by specific fetal hair follicle growth factors.
Results of our patients’
To prevent disease and delay commencement of aging
Program of rejuvenation “Golden Vial” successfully approved in Ukraine and Russia.
With the help of “Golden Vial” many famous people received rejuvenation. Below we
have shown average statistics data of rejuvenation results in 365 people (247 female
and 138 male) and likewise quoting evaluation of patients who has completed
program of “Golden Vial”.

General Characteristics of patients, participated in study (before treatment)
Age group
Возрастные
группы
Female, n = 247 Male, n = 138
CA,
Yrs
BA
Yrs
CA – BA
± Yrs
FI
Units
CA,
Yrs
BA
Yrs
CA – BA
± Yrs
FI
Units
30-39 yrs
34.8±1.5
n = 56
40.3±1.7
n = 56
+5.5±0.8
n = 56
0.33±0.02
n = 56
35.4±1.6
n = 22
41.4±1.8
n = 22
+6.0±0.6
n = 22
0.35±0.03
n = 22
40-49 yrs
45.3±1.9
n = 103
52.6±2.0
n = 103
+7.3±0.5
n = 103
0.38±0.03
n = 103
46.1±1.5
n = 75
53.6±1.7
n = 75
+7.5±0.4
n = 75
0.41±0.02
n = 75
50-59 yrs
56.5±2.1
n = 65
66.0±2.4
n = 65
+9.5±0.7
n = 65
0.42±0.03
n = 65
53.8±2.3
n = 24
63.6±2.9
n = 24
+9.8±0.5
n = 24
0.47±0.04
n = 24
60-69 yrs
63.3±2.5
n = 23
74.1±2.7
n = 23
+10.8±0.9
n = 23
0.47±0.04
n = 23
64.2±2.7
n = 23
75.6±3.1
n = 23
+11.4±0.8
n = 23
0.52±0.04
n = 23
70-80 yrs
72.2±3.0
n = 9
83.5±3.2
n = 9
+11.3±1.2
n = 9
0.53±0.04
n = 9
71.9±3.3
n = 7
83.9±3.5
n = 7
+12.0±0.9
n = 7
0.58±0.05
n = 7
CA – Chronological age. BA – biological age. CA – BA – difference between
biological and chronological age. FI - Frailty Index

Frequency of aging symptoms among all study patients of all age group (%)
Symptoms F M Symptoms F M
Presence of grey hairs 44 47 Lip chapping 24 25
Presence of baldness, less dense hair
growth on scalp
52 59 Frequent (2-3 times/year) herpes on
mouth
48 39
Hair fall 26 5 Symptom "worm" – a throbbing,
thickening of temporal artery
41 78
Symptom of diagonal folds of ear 95 98 Symptom "red drops" – hemangiomas on
the body, telangiectasia, spider veins
80 97
Wrinkles on the face and neck 50 57 Symptoms of leukolonychia (white spots
on the nails)
55 46
Xanthelasmatosis 8 29 Face asymmetric 5 10
Symptom of rabbit eye (sclera injection) 6 30 Dryness of skin, edema and paleness of
skin
11 17
Pigment dots and appearance of age senile
nevus
37 51 Symptom "dark", scaly hands and knees 15 21
Scanty hairs in eyebrows and eyelashes 75 88 Hyper-pigmentation of orbit skin, palmar
creases
2 9
Imprints of teeth in the tongue 3 15 Impaired smooth speech 14 7
Coating on tongue, “geographic tongue” 9 13 Hyperemia of palms and soles 36 47

Frequency of aging diseases among all study patients of all age group (%)
Aging Disesaes F M Aging Disesaes F M
Atherosclerosis 53 79 Immunodeficiency 69 53
Arterial hypertension 32 66 Osteoporosis 78 24
Arterial hypotonic 57 5 Joint diseases 68 36
Ischemic cardiac disease 5 13 Diabetes Mellitus 9 6
Heart failure 3 7 Weakness of hearing and vision 61 67
Post stroke condition 4 2 Asthenia syndrome 71 19
Autoimmune disease 45 44 Depression 46 12

Characteristics of morphological type of aging of the face in study groups of
female
Type of aging Signs Number of
observation
%
Fatigue morpho-
type
Reduction of tone in the smooth tissues of the face and muscles,
dry and dull skin. Early appearance of folds and wrinkles,
deepening of naso-labial folds, lowering of mouth corners and
appearance of wrinkles there (wrinkles “puppets”), lacrimal
groove.
157 63.6
Wrinkles morpho-
type
Wrinkles due to thinning of skin, dry, prone to irritation and
redness of skin. Poor contours saggy smooth tissue of face.
59 23.9
Deformative
morpho-type
Puffiness of face tissue, flabby pore skin. Redness of cheek with
deformities face contours, second chin formation, “flew”, neck
folds, eye bags and droopy eyelids.
28 11.3
Muscular morpho-
type
Disturbance in pigmentation, folding of upper and lower
eyelids, pronounced naso-labial folds. Skin of cheek is flat and
smooth. Contours of oval face save up to old age.
3 1.2

Gerontology and clinical-instrumental analysis was performed after 6, 12, 18, 24, 36, 42, 48,
54 and 60 months after patients undergoing of full program of “Golden Vial”:
Evaluation of viscoelastic parameter of hand skin method of cutometer (Cutometer, Courage
& Kazaka, Germany). Method based on the principle of vertical deformation. Parameter: F –
maximum level of skin deformation; U – elasticity of skin.
0
20
40
60
80
100
0 6 12 18 24 30 36 42 48 54 60
units
months
F
U
Dynamics of patient’s cutometer parameter after undergoing complete program of
“Golden Vial”
Result: Maximum level of the skin deformation reduced parallel to increase its
elasticity in whole observation period.

Clinical evaluation conducted by the following features: roughness, radiance and
uniformity of hand skin tone (by scale in points).
0
2
4
6
8
10
0 6 12 18 24 30 36 42 48 54 60
units
months
Dynamics of parameters of the smoothness (green line), radiance (brown line)
and uniformity of hand skin tone (blue line) after patients undergoing complete
program “Golden Vial”

Evaluation of results. Evaluation results of skin rejuvenation of hand, neck, décolleté
and face of doctors and patients conducted based on the fundamental international
evaluation scale GAIS (Global Aesthetic Improvement Scale):
Points Evaluation of doctors Evaluation of patients
3 Optimal cosmetic results for the given
patient
Complete satisfaction of results
2 Significant improvement, but not
complete correction
Satisfied of the results, but wanted to
improve it little more
1 Improvement, but required to additional
correction
Improvement insignificant, desirable to
additional correction
0 No changes, condition similar as before
procedure
No changes
−1 Deterioration in comparison with the
initial condition
Condition is worse than before
performing procedure

.Evaluation of doctors:
Optimal cosmetic results for the given patient - 198 (80.2%)
Significant improvement but not complete correction – 21 (8.5%)
Improvement, but required additional correction – 28 (11.3% –
practically all women with deformities type of face of aging)
No changes, condition same as before procedure – 0
Deterioration in comparison to initial condition – 0
Evaluation of patients:
Completely satisfied of results – 195 (78.9%)
Satisfied of the results, but wanted to improve it little more – 24 (9.7%)
Improvement insignificant, desirable for additional correction – 28
(11.3% – practically all women with deformities type of face of aging)
No changes – 0
Condition worse, than before performing procedure – 0

Dynamics of Frailty Index in female and male age group of 30-39 yrs
after patients undergoing complete program “Golden Vial”

Dynamics of Frailty Index in female of all age group after patients
undergoing complete program “Golden Vial”
0
0.1
0.2
0.3
0.4
0.5
0.6
0 6 12 18 24 30 36 42 48 54 60
FrailtyIndex,units
months
30-39yrs
40-49yrs
50-59yrs
60-69yrs
70-80yrs

Dynamics of Frailty Index in male of all age group after patients
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0 6 12 18 24 30 36 42 48 54 60
FrailtyIndex,units
months
30-39 yrs
40-49 yrs
50-59 yrs
60-69 yrs
70-80 yrs

.Conclusion: better results observed in patients of
age 30-39 yrs. Consequently earlier the
rejuvenation program started “Golden Vial”, lesser
the probability of aging disease development. But
even in patients of age groups 70-80 yrs our
program significantly reduces Frailty index that
evidences about the decrease in risk of aging
diseases appearance.

.Dynamics of difference between biological and chronological age
parameters in female and male age group of 30-39 yrs after patients

Dynamics of difference between biological and chronological age

parameters in female of all age group after patients undergoing
complete program “Golden Vial”
-20
-15
-10
-5
0
5
10
15
0 6 12 18 24 30 36 42 48 54 60
±yrs
months
30-39 yrs
40-49 yrs
50-59 yrs
60-69 yrs
70-80 yrs

parameters in male of all age group after patients undergoing complete
program “Golden Vial”
-20
-15
-10
-5
0
5
10
15
0 6 12 18 24 30 36 42 48 54 60
±yrs
months
30-39 yrs
40-49 yrs
50-59 yrs
60-69 yrs
70-80 yrs

.
Conclusion: Dynamic changes of difference between biological and
chronological age evidences that with the help of rejuvenation program
“Golden Vial” true rejuvenation is reached.
Maximum result in the age group for female:
30-39 yrs – to 15.6 yrs
40-49 yrs – to 15.8 yrs
50-59 yrs – to 13.6 yrs
60-69 yrs – to 10.8 yrs
70-80 yrs – to 4.9 yrs
Maximum result in the age group for male:
30-39 yrs – to 16.4 yrs
40-49 yrs – to 16.5 yrs
50-59 yrs – to 12.6 yrs
60-69 yrs – to 11.7 yrs
70-80 yrs – to 5.4 yrs
Thus, observing these above data you could anticipate your rejuvenation
after undergoing complete rejuvenation program “Golden Vial”.

.
Case History (Female)
Female, 37 yrs, actress of theatre and movie. “Approached us and said I am scared
of aging. I have problem with face, neck, hand, and breast skin. Hair is like oakum,
falling – morning whole comb “hairy”. Nails are simply terrible, hands have some
small pale brown pigmentation, wrinkles in fine mesh, often appearance of chapping.
Off late cannot read anything without spectacles, cannot hear the interlocutor while
talking in mobile phone but better in the left than the right ear therefore I hold it
away. Volatile pain in the small joints of hands and leg, by the mid day I have
“crumpled face”, and most important I noticed that my cream and other cosmetic
products does not help. In shooting I become tired easily and even could break out
with tantrum. Friends tell that I have become easily resentful, tearful, irritable and
rude. I have frequent ailments and cold, menstrual cycle is disturbed. I am sleepless
at nights and sleepy in the noon. I have reduced libido and scandals with husband.
Shout at children, dry skin and when I panic and worry become sweaty and tremor of
hands. Sweat smells very unpleasant. Feel often tiredness. Dress does not fit already;
I need to change the wardrobe. In the mirror to see myself very scary: wrinkles,
cellulites, simply terrible. I don’t want to see anyone, don’t to meet school friends,
birthdays, marriages, parties, chat with friends. I have often depression, I started to
smoke, consume alcohol often to remove stress and aging. I am just 37 please help
me!”

.
Result of complete medical analysis of patients. Biological age exceeds chronological age
on 9 years. Disturbances were identified in cardiovascular system (arterial hypotonic), joints
(polyarthritis), immune system (T-cell deficit) and endocrine (unbalanced production of
estrogen and progesterone). Frailty Index – 0.375.
Gerontological diagnosis: Early (accelerated) aging [+9 yrs – 37 yrs wear of functional
system of patient corresponds to 46 yrs, early pathological climacteric status], type of
combined aging (cardio-vascular, endocrine and neuro-psycho-somatic).
Treatment: complete course “Golden Vial
Treatment Results:
2005 yr 2006yr 2007 yr 2008 yr 2009 yr 2010 yr
BA>CA= + 7yrs
Frailty Index =
0.375
Undergone
complete
program of
“Golden Vial”
BA>CA= + 2yrs
Frailty Index =
0.275
Improvement of
condition of
functional system
of menstrual
cycle-normal
BA<CA= − 1yr
Frailty Index
=0.050
Functional
systems - normal
External
appearance –
normal for the
current age
menstrual cycle –
normal
BA<CA= − 3yr
Frailty Index
=0.025
Functional
systems - normal
External
appearance -
rejuvenation
menstrual cycle –
normal
BA<CA= − 5yr
Frailty Index
=0.025
Functional
systems - normal
External
appearance -
rejuvenation
menstrual cycle –
normal
BA<CA= − 5yr
Frailty Index
=0.025
Functional
systems - normal
External
appearance -
rejuvenation
menstrual cycle –
normal

Case History (Male)
Male, 42 yrs, successful business men. “One day I woke up and I noticed in the
mirror my moustache and temple region got grey hairs. Took second small mirror
and found that in the region of crown scanty hair and began to take shape of bald. I
changed my hair style, started to comb from forehead to back so that to hide
thinning of hair. Started to analyze whole health condition and surprised of aging
signs. First of all, potency and interest in women indifference to sex has reduced. I
became irritable. Surprised unpleasantly that redness of face appears during
pressurized work after meeting or important conversations. It warned me that, by
the end of the day face remained red, irritable, feeling of unreasoning anxiety and
even fear. Unpleasant odor in mouth from the morning appears to be even though
last evening didn’t drink alcohol. Started to get tired faster at work and in sports
hall, muscle strength has gone down. I decreased interest to work and success.
Weakness and apathy period has started. That why I have approached your help”.
Result of complete analysis of patient. Biological age exceed the chronological age
by 13 years. Disturbances were identified in cardiovascular system (arterial
hypertension), liver (reduced detoxification function) and endocrine system
(reduced production of testosterone and insulin). Frailty Index – 0.275.

Gerontological diagnosis: Early (accelerated) aging [+13 yrs – in 42 yrs wear of
functional system of patient corresponds to 55 yr, onset of male climacteric status],
type of combined aging (cardio-vascular, endocrine and neuro-psychological).
Treatment: complete course “Golden Vial”.
Treatment results:
2005 yr 2006 yr 2007 yr 2008 yr 2009 yr 2010 yr
BA>CA = + 13 yr
Frailty Index =
0.275
Undergone
complete program
of “Golden Vial”
BA=CA
Frailty Index =
0.125
Improvement of
condition of
functional system
BA<CA= − 3 yr
Frailty Index
=0.050
Functional
systems - normal
Patient married
BA<CA= − 5 yr
Frailty Index
=0.025
Functional
systems – normal
Patient had male
child
BA<CA= − 6 yr
Frailty Index
=0.025
Functional
systems – normal
BA<CA= − 6 yr
Frailty Index
=0.025
Functional
systems – normal

.SUMMARY
Aging – this is decrease of stem cells reserve in organs and human tissues.
For true rejuvenation need to increase stem cells reserve in human organs and
tissues.
Aging Disease: Arthrosclerosis and arterial hypertension, Chronic ischemic heart
disease, myocardial infarction, heart failure, stroke, cancer, autoimmune disease,
pneumonia and respiratory failure, stomach ulcers and duodenal bleeding,
perforation, penetration and malignant diseases, blindness, deafness, fracture,
osteomalacia, joint diseases, renal disease, liver, diabetes mellitus, asthenia,
depression, senile dementia, Alzheimer’s disease.
Reason for aging diseases: reason of aging disease is as aging itself the reduction of
stem cells reserve in organs and human tissues.
In prophylactics of aging diseases stem cells reserve is required to be increased in
organs and tissues of the human.
Aging – it is decrease of stem cells reserve in organs and tissues of the human.
For true rejuvenation need to increase stem cells reserve in human organs and
tissues.
Chronological age – this is age as per birth of date.

.Biological age – this is “level of age wear” of our body.
Standard quantitative characteristic of aging is difference between
biological aging and chronological aging.
Standard qualitative characteristic of aging is Frailty Index evaluating
age damage and deficit function. More higher the Frailty Index lesser the
quality of life and life span.
Important indicator of true rejuvenation – period of healthy life.
Successful aging – this is absence of disease and physical disability,
continued ability to assimilate new information and physical activity,
active part in family life and society. Successful aging – this is active
longevity
“Golden Vial” – it is program is transplantation of all types stem cell
without any exception which is necessary for complete renewal of
functional system and rejuvenation. Program includes 240 types of fetal
stem cells transplantation and special complex bio-creams which
provides regeneration of hair, skin and nails

.Results of rejuvenation of the hand, neck, décolleté and face skin in
women, undergone program “Golden Vial”, according to international
assessment scale GAIS (Global Aesthetic Improvement Scale):
Evaluation of doctor/patient:
Optimal cosmetic result of the given patient – 80.2/78.9%
Significant improvement but not complete correction – 8.5/9.7%
Improvement but additional correction required – 11.3/11.3% (women
with deformities type of aging in face)
No changes condition is same as before procedure – 0/0
Deterioration in comparison with initial condition – 0/0
Earliest to start the rejuvenation program “Golden Vial” the lesser
probability of aging disease development.

.Anticipated result of true rejuvenation with the help of
rejuvenation program “Golden Vial”:
For women :
30-39 yrs – to15.6 yrs younger
40-49 yrs – to 15.8 yrs younger
For men:
70-80 yrs т – to 5.4 yrs younger

.Our publication
THE AGING: THEORY OF THE BODY STEM-SPACES DEPLETION
O.L. Kukharchuk, V.V. Radchenko, B.M. Gandhi, A.B. Padma Priya, A.O.
Kukharchuk, Amol P. Gitte
EmProCell Clinical Research Pvt. Ltd., Mumbai, India
The review contains an analysis of basic information about the principles
and methods of a new direction in the treatment of human diseases
named as regenerative medicine, basic information on stem-spaces of
organism, provides information on the depletion of stem cells resources
in the aging, and presented authors own theory of the aging
mechanisms.
Key words: stem cell, stem-spaces, immune system replacement, aging,
regenerative medicine.

.
Kukharchuk O.L., Radchenko V.V., Gandhi B.M., Padma Priya A.B., Kukharchuk A.O., Amol P. Gitte. “The aging: theory
of the body stem spaces depletion” (2011) Medicine Today and Tomorrow 50-51 (1-2) 292-310 (Eng).
Kukharchuk A.L., Radchenko V.V. “Embryonic pluripotent progenitor cells, immunological tolerance, and aging of
organisms” (2008) Transplantology 10 (1) 51-54 (Rus).
RadchenkoV.V., Kukharchuk A.L., Sirman V.M. “Embryonic pleuripotent progenitor cells, immunological tolerance,
autoimmune diseases and aging of organism”(2003) Transplantology 4 (1) 225–228 (Rus).
Radchenko V.V., Kukharchuk A.L. “Influence of embryonic pleuripotent progenitor cells on intensiveness and structure
of tissue fibrinolysis in the aorta of old rats” (2003) Herald of Biology and Medicine Problems 5 (3) 53-56 (Rus).
Radchenko V.V., Kukharchuk O.L. “Influence of embryonic pluripotent progenitor cells on proteolysis intensiveness in
old rats aorta tissues” (2003) Medical Chemistry (21) (9) 32-37 (Ukr).
Radchenko V.V., Kukharchuk A.L. “Transplantation of allogenic embryonic pleuripotent progenitor cells for the
correction of arterial pressure regulation dysfunction: Assesment of effectiveness in experiment on rats with
genetically determined arterial hypertension like position of theory of stem space organism depletion” (2003)
Medicine Today and Tomorrow (7) 15-22 (Rus).
Kukharchuk A.L., Radchenko V.V., Sirman V.M. “Aging, stem spaces and immune system” (2003) International Journal
Immunorehabitation 5 (2) 134 (Rus).
Kukharchuk O., Radchenko V.V., Padma Priya A.B., Kukharchuk A.O., Pranav Anam “Stem cell therapy in nephrology”
(2012) Medi-focus 12 (1) 1-7 (Eng).
Kukharchuk O., Salyutin R., Kukharchuk A., Radchenko V, Anand Baskaran Padma Priya, Pranav Anam “ Efficiency of
human fetal liver hematopoietic stem cells (hFL-HSC) transplantation in treatment of chronic limbs ischemia (CLI)”
(2012) J Cell Sci Ther 3 (8) 59 (Eng).
Kukharchuk O.L. “Ukrainian experience of fetal stem cells transplantation for severe human diseases treatment: 13
years of clinical applications in legal field” (2012) J Cell Sci Ther 3 (8) 27 (Eng).
Kukharchuk A.L., Palaynica S.S., Bikhunov V.L. “Diabetes mellitus, regenerative medicine and immunological
tolerance” (2008) Transplantology 10 (1) 44-50. (Rus).

.Kukharchuk A.L., Radchenko V.V. “Influence of embryonic progenitor cells transplantation on the reproductive
system of old male rats” (2007) Transplantology 9 (1) 142-146 (Rus).
Skavulyak A.N., Kukharchuk A.L. “The study of mechanism neurogenesis dysfunction as the cell therapy basis for
schizophrenia” (2007) Transplantology 9 (1) 263-269 (Rus).
Kukharchuk O.L., Ganja T.M. “Stem cells of fetal liver: identification problem and prospects of application in
practical medicine” (2007) Transplantology 9 (1) 147-150 (Ukr).
Kukharchuk A.L., Palyanica S.S. “Theoretical basis for neutral stem cells application in treatment of the brain
ischemic lesions” (2007) Transplantology 9 (1) 203-208 (Rus).
Kukharchuk A.L., Radchenko V.V., Sirman V.M. “Evaluation of effectiveness of various types of embryonic brain cells
transplantation in rats with experimental mode of hemi-Parkinson’s” (2004) Transplantology (7) (No.3) (Rus).
Kukharchuk A.L., Radchenko V.V., Sirman V.M. “Regenerative medicine: Directions, achievements problems and
prospects. Part IІ: Stem spaces” (2004) Ukrainian Medical Annals 41 (3) 99-107 (Rus).
Kukharchuk A.L., Radchenko V.V., Sirman V.M. “Regenerative medicine: Directions, achievements problems and
prospects. Part I: Principles and Methods” (2004) Ukrainian Medical Annals 40 (2) 70-79 (Rus).
Kukharchuk A.L., Radchenko V.V., Sirman V.M. “Stem cells: experiment, theory, clinic. Embryonic, mesenchymal,
neural, and hematopoetic stem cells” (2004) Chernivtsi: Gold Litavry, 505 (Rus).
Kukharchuk O.L., Radchenko V.V., Sirman V.M., Sagach V.F. “Influence of allogenic embryonic pleuripotent
progenitor cells transplantation on the systemic arterial pressure dynamic in spontaneous hypertensive rat” (2003)
Journal Physiology, 49 (4) 68-72 (Ukr).
Kukharchuk A.L., Radchenko V.V., Sirman V.M., Radchenko M.R., Dyba S.A. “Achievements, problems and prospects
of regenerative medicine” (2003) Transport Ukraine Medicine 7 (3) 87-100 (Rus).

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