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LINKAGE AND CROSS OVER
1
Polytene Chromosome
2
LINKAGE
3
F
eE
cC
bB
aA
f
4
F
eE
cC
f
B
A
5
Thomas Hunt Morgan
6
7
Red - dominant (R)
Purple - recessive (r)
Normal wings - dominant(N)
Vestigial wing - recessive (n)
Eye colour
Type of wing
8
9
Normal Vestigial
10
RrNn x rrnn
RRNN X rrnn
RrNn
RN rn
Parent
Gamete
F1
Test cross
11
Expected results were:
25% Red Normal
25% Red vestigial
25% purple Normal
25% purple vestigial
The actual results were:
47% Red Normal
43% Purple vestigial
5% Red vestigial
5% purple normal
12
Types of linkage
1.Complete linkage
Closely associated
Pass to 2/ more generations
2.Incomplete linkage
Widely located
High chance of separation by cross over
13
CROSS OVER
14
15
16
Crossover is the process by which two chromosomes paired up during
prophase I of meiosis exchange a distal portion of their DNA
Crossover occurs when two chromosomes, normally two homologous
instances of the same chromosome, break and connect to each other's
ends
If they break at the same locus, this merely results in an exchange of
genes
This is the normal way in which crossover occurs
17
If they break at different loci, the result is a duplication of genes on one
chromosome and a deletion on the other
If they break on opposite sides of the centromere, this results in one
chromosome being lost during cell division
Any pair of homologous chromosomes may be expected to cross over three or
four times during meiosis
This aids evolution by increasing independent assortment, and reducing the
genetic linkage between genes on the same chromosome
18
Dihybrid cross – one point
19
Trihybrid cross – 2 point
20
Types of cross over product
1. Parental/ Non cross over type
Coupling phase
2. Recombinant/ Cross over type
Repulsion phase
21
Genetic map
Any method used for determining the location of and relative
distances between genes on a chromosome
Map distance =
Number of recombinant offspring
Total number of offspring
× 100
Map unit – centiMorgan (cM)
1 Map unit = 1% recombination frequency
22
 Parental phenotypes – most frequently
 Double cross over – least frequently
 Single cross over – Intermediate frequently
Variation in the
number of chromosomes
Variation in the
arrangement of chromosomes
32
Why it is not possible to use a homozygous dominant organism
(such as TT) in a test cross experiment to determine the
genotype of an organism showing the dominant phenotype.
Illustrate your answer with fully using appropriate genetic
symbol?
33
In maize the following cross between pure breeding plants were made,
coloured shrunken starchy × colourless full waxy. The F1 plants were all
found to be coloured full starchy. The F1 plants were then test crossed
and the following progenies were obtained. Explain the results and
obtain the necessary information regarding the inheritance of these
characters.
Coloured shrunken starchy 2538
Colourless full waxy 2708
Coloured full waxy 116
Colurless shrunken starchy 113
Colured shrunken waxy 601
Colourless full starchy 626
Colured full starchy 4
Colourless shrunken waxy 2

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2015 03-13 Linkage and Crossover

  • 7. 7
  • 8. Red - dominant (R) Purple - recessive (r) Normal wings - dominant(N) Vestigial wing - recessive (n) Eye colour Type of wing 8
  • 9. 9
  • 11. RrNn x rrnn RRNN X rrnn RrNn RN rn Parent Gamete F1 Test cross 11
  • 12. Expected results were: 25% Red Normal 25% Red vestigial 25% purple Normal 25% purple vestigial The actual results were: 47% Red Normal 43% Purple vestigial 5% Red vestigial 5% purple normal 12
  • 13. Types of linkage 1.Complete linkage Closely associated Pass to 2/ more generations 2.Incomplete linkage Widely located High chance of separation by cross over 13
  • 15. 15
  • 16. 16 Crossover is the process by which two chromosomes paired up during prophase I of meiosis exchange a distal portion of their DNA Crossover occurs when two chromosomes, normally two homologous instances of the same chromosome, break and connect to each other's ends If they break at the same locus, this merely results in an exchange of genes This is the normal way in which crossover occurs
  • 17. 17 If they break at different loci, the result is a duplication of genes on one chromosome and a deletion on the other If they break on opposite sides of the centromere, this results in one chromosome being lost during cell division Any pair of homologous chromosomes may be expected to cross over three or four times during meiosis This aids evolution by increasing independent assortment, and reducing the genetic linkage between genes on the same chromosome
  • 20. 20 Types of cross over product 1. Parental/ Non cross over type Coupling phase 2. Recombinant/ Cross over type Repulsion phase
  • 21. 21 Genetic map Any method used for determining the location of and relative distances between genes on a chromosome Map distance = Number of recombinant offspring Total number of offspring × 100 Map unit – centiMorgan (cM) 1 Map unit = 1% recombination frequency
  • 22. 22  Parental phenotypes – most frequently  Double cross over – least frequently  Single cross over – Intermediate frequently
  • 23.
  • 24. Variation in the number of chromosomes
  • 25.
  • 26.
  • 27.
  • 29.
  • 30.
  • 31.
  • 32. 32 Why it is not possible to use a homozygous dominant organism (such as TT) in a test cross experiment to determine the genotype of an organism showing the dominant phenotype. Illustrate your answer with fully using appropriate genetic symbol?
  • 33. 33 In maize the following cross between pure breeding plants were made, coloured shrunken starchy × colourless full waxy. The F1 plants were all found to be coloured full starchy. The F1 plants were then test crossed and the following progenies were obtained. Explain the results and obtain the necessary information regarding the inheritance of these characters. Coloured shrunken starchy 2538 Colourless full waxy 2708 Coloured full waxy 116 Colurless shrunken starchy 113 Colured shrunken waxy 601 Colourless full starchy 626 Colured full starchy 4 Colourless shrunken waxy 2