Ponencia: Instructions for the collection of plant specimens and endophytes studies
Autor: Dr. Gary Strobel
Evento TF Innova:
Workshop Biotechnology "Isolation and identification of endophytic fungi from vascular plants"
24. The PTR – MS of Standard VOCs of M. albus
Compound Major ion and % in ( )
Acetic acid, 2-phenylethyl 43 (37), 61 (63)
ester
Phenyl ethyl alcohol 105 (100)
Propanoic acid, 2-methyl, 41 (7), 43 (18), 57 (35), 89 (31), 145 (9)
2-methylpropyl ester
Propanoic acid, 2-methyl, 43 (39), 71 (42), 89 (16), 159 (3)
3-methylbutyl ester
Propanoic acid, 2 methyl, 43 (4), 89 (11), 117 (85)
ethyl ester
Propanoic acid,2-methyl 41 (11), 43 (42), 89 (47)
Propanoic acid, 2-methyl, 43 (3), 103(97)
methyl ester
1-Butanol, 3-methyl, 41 (10), 43 (47), 71 (37), 131 (4)
acetate
25. 34
Temperature
800
33
mass 131
32
Temperature (C)
600
Intensity (cps)
31
400
30
mass 103 29
200
Mass 131= Ca. 18ppb 28
Mass 103=Ca. 12ppb
0
14 15 16 17 18 19
Time (days)
47. Effects of B-23 on human pathogenic bacteria
Organism Type of Exposure Growth/ No Comments
Cell Wall Time Growth
(in the presence of M.
crispans)
S. aureus 6538 Gram + 2, 4 and 6 No growth
days
S. cholerasuis 10708 Gram - 2, 4 and 6 No growth
days
P. aeruginosa 15442 Gram - 2 days Growth No visible difference between
exposed and control plates.
S. aureus ATCC 43300 Gram + 2, 4 and 6 Growth No actual colonies formed,
(MRSA) days just a slightly filmy growth.
Y. pestis 91-3365 Gram - 3 and 5 days No growth
B. anthracis A2084 Gram + 3 and 5 days Growth Only a few colonies left after
exposure and when
incubated, more grew.
M. tuberculosis 3081 Acid-fast 2, 4, 7 and 14 No growth
(resistant to isoniazid) days
M. tuberculosis 50001106 Acid-fast 2, 4, 7 and 14 No growth
(resistant to streptomycin) days
M. tuberculosis 59501228 Acid-fast 2, 4, 7 and 14 No growth
(resistant to days
streptomycin/ethambutol)
M. tuberculosis 59501867 Acid-fast 2, 4, 7 and 14 No growth
(susceptible) days
50. Lessons Learned from M. albus and its
relatives
1. Each has 95% -99% 18S rDNA partial sequence
similarity to the original isolate of M. albus -620.
2. They make different VOC’s and in differing amounts.
3. They are found as endophytes in the wet tropical
regions of the world from Thailand, to Indonesia,
Australia, Venezuela, and Peru. They are confined to +/-
16 degrees from the equator.
4, Each has some type of bioactivity.
5. Some are being developed for commercial purposes.
6. Many other surprises remain in the jungle.