10. EmbrionariasNO USAMOSs Blástula Desarmar el embrión In 1998, researchersfromtheuniversity of Wisconsin isolatedstemcellsfromIVF-blastocysts.
11.
12. Problemas para el uso clínico de células madre embrionarias Capacidad de inducción de tumores Rechazo (diferencias en los antígenos de histocompatibilidad entre embrión y receptor) Necesidad de inmunosupresión o clonación Necesita mucha tecnología (mayor costos para el manejo)
15. Fuentes de CM Adultas Médula Ósea: Múltiples tipos de células madre Sangre venosa periférica: las células son movilizadas hacia la sangre periférica utilizando Factores de crecimiento (G-CSF) y luego colectadas por aféresis Sangre de Cordón Umbilical: “semejantes” a las de la MO, son colectadas al momento del parto Líquido amniótico Placenta Grasa
21. Hematopoyética VSEL MIAMI Mesenquimal C. Progenitoras Endoteliales MAPC MIAMI VSEL CPE SC mesenquimales A population of very small embryonic-like (VSEL) CXCR4þSSEA-1þOct-4þ stem cells identified in adult bone marrow M Kucia, R Reca, FR Campbell, E Zuba-Surma, M Majka, J Ratajczak and MZ Ratajczak Hemangioblatos MAPCs
22. Preliminary Evidence of Plasticity Among Nonhuman Adult Stem Cells. NIH USA T D T D D Diferenciación D T D T Transdiferenciación
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24. Bibliografía Hematopoietic cells differentiate into both microglia and macroglia in the brains of adult mice Eglitis MA, et al. Proc Natl Acad Sci U S A 1997;94:4080-5 Muscle regeneration by bone marrow-derived myogenic progenitors Ferrari G, et al.Science 1998;279:1528-30 Turning brain into blood: a hematopoietic fate adopted by adult neuronal stem cells in vivo Bjornson CR, et al.Science 1999;283:534-7 Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow Mezey E, et al.Science 2000;290:1779-82 Purified hematopoietic stem cells can differentiate into hepatocytes in vivo Lagasse E, et al. Nat Med 2000;6:1229-34 Allogeneic mesenchymal stem cells restore cardiac function in chronic ischemic cardiomyopathy via trilineage differentiating capacity Joshua M. Harea PNAS, August 18, 2009, vol. 106 no. 33, p. 14022–27
25. Theise ND, et al. Hepatology 2000Alison MR, et al. Nature 2000 Hepatocito XX Evidencia en seres humanos Trasplantes de MO Receptor Receptor Donante XY XY XX
26. Cardiomiocito Laflamme MA, et al. Circ Res 2002Quaini F, et al. N Engl J Med 2002Minami E, et al. Circulation 2005 Evidencia en seres humanos Trasplantes de órganos sólidos Receptor Donante Donante XY XX XY XY
27. Células Madre que normalmente salen y migran hacia tejidos lesionados, para ayudar en la reparación de tejidos. HOMING Guiadas por señales químicas de inflamación. AMPLIAR este mecanismo. Células madre ADULTAS AUTÓLOGAS con mínima manipulación Médula Ósea
32. Allogeneic mesenchymal stem cells restore cardiac function in chronic ischemic cardiomyopathy via trilineage differentiating capacity. Joshua M. Harea, PNAS, August 18, 2009, vol. 106 no. 33, p. 14022–27 Perpetuán el estímulo
33. Stem cells show potential for many different areas of health and medical research, and studying them can help us understand how they transform into the dazzling array of specialized cells that make us what we are. Some of the most serious medical conditions, such as cancer and birth defects, are caused by problems that occur somewhere in this process. A better understanding of normal cell development will allow us to understand and perhaps correct the errors that cause these medical conditions. Research on one kind of stem cell—human embryonic stem cells—has generated much interest and public debate. Pluripotent stem cells (cells that can develop into many different cell types of the body) are isolated from human embryos that are a few days old. Pluripotent stem cell lines have also been developed from fetal tissue (older than 8 weeks of development). As science and technology continue to advance, so do ethical viewpoints surrounding these developments. It is important to educate and explore the issues, scientifically and ethically. For More Information: The following links to other Internet sites are offered only for the convenience of World Wide Web users. The NIH is not responsible for the availability or content of these external sites, nor does the NIH endorse, warrant, or guarantee the products, services, or information described or offered at these other Internet sites. Research Ethics and Stem Cells
34. Presidential Commission on Bioethics The Presidential Commission for the Study of Bioethical Issues advises President Obama on bioethical issues that may emerge from advances in biomedicine and related areas of science and technology. National Academy of Sciences Guidelines for Human Embryonic Stem Cell Research The National Academy of Sciences developed its first set of ethical standards for stem cell research in 2005. The guidelines were updated in 2007, 2008, and 2010. The Kennedy Institute of Ethics at Georgetown University Library & Information Services allows searches of books, newspapers, journal articles, and other materials on bioethical issues. Stem Cell Research and Applications: Monitoring the Frontiers of Biomedical Research(300K PDF; get Adobe Reader) The American Association for the Advancement of Science (publisher of Science magazine) and the Institute for Civil Society produced this report addressing stem cells and ethics. The Ethics of Human Embryonic Stem Cell Research The International Society for Stem Cell Research provides this information as a public service to those wishing to discuss stem cell ethics. Report On Human Embryonic Stem Cell Research from the European Union (1.5MB PDF; get Adobe Reader) Bioethics Advisory Committee (BAC) Singapore Addresses the ethical, legal, and social issues arising from biomedical sciences research.
41. STROKE – ACV – Infarto CerebralHipoxia cerebralFalta de oxígeno en cerebro
42. EXPERIENCIA INFARTOS CEREBRALES: -1 pacientes con 18 años de infarto -1 paciente con 4a de infarto cortical -1 paciente con 6 meses de ACV -1 paciente con 3 meses de infartotroncal
45. Estudios futuros son necesarios para evaluar la eficiencia del implante de celulas madre.
46. La presencia de células madre marcada con TC 99m a las 4 horas después del trasplante sugiere pasaje de estas células por la barrera hematoencefálica
47. La imagen PET-FDG sugiere la presencia de células madre metabólicamente vivas a los 7 dias post implante de las Células Madre Dr. Hans Dohman - Brazil II Congreso de Terapia Celular del Pacífico Sur Agosto 2006 Lima, Perú
48. SPECT Cerebral BASAL 7 DIAS POST 6 MESES POST 5to dia post infarto 7imo dia post implante CM Manejo del Stroke Agudo con Stem Cells 7mo dia post implante CM 5to dia post infarto 16avo dia post implante CM Dr. Hans Dohman - Brazil II Congreso de Terapia Celular del Pacífico Sur Agosto 2006 Lima, Perú
51. CIRROSISALTERNATIVAS DE TRATAMIENTO Eliminar las causas. Tratar cada síntoma. Trasplante de hígado. TIPS PARACENTESIS SHUNT PERITONEO VENOSO EMBOLIZACIÓN DE VÁRICES ESOFÁGICAS
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