1. THAMEEM AZARUDEEN. R, M.SC., M.PHIL.
51/18, KAYITHE MILLATH STREET, ARUPPUKOTTAI-626101, TAMIL NADU, INDIA.
e-mail: thameemrmsait@gmail.com • CELL: 8438123397, 9442059439.
EDUCATIONAL SUMMARY
S.No Year Name of the
course
Name of the Institution Percentage of
marks
1 2013-
Present
Ph.D. Khadir Mohaideen college,
Adirampattinam, Tamilnadu.
Thesis in
progress
2 2011-2013 M.Phil.,
Zoology
Jamal Mohamed College,
Trichy, Tamilnadu.
73
3 2009-2011 M.Sc., Zoology Khadir Mohaideen college,
Adirampattinam, Tamilnadu.
69
4 2006-2009 B.Sc., Zoology SBK College,
Aruppukottai, Tamilnadu.
61
5 2004-2006 H.Sc Al Ameen Muslim Higher secondary school,
Aruppukottai, Tamilnadu.
60
EDUCATION AND TRAINING
PH.D., STUDENT AND TEACHING ASSISTANT – (August 2013 - present):
BHARATHIDASAN UNIVERSITY, KHADIR MOHAIDEEN COLLEGE, DEPT. OF. ZOOLOGY,
TAMILNADU. (GUIDE: DR. A. AMSATH, ASSOCIATE PROFESSOR)
PhD Thesis: Investigate the larvicidal, pupicidal and repellant effects of mangrove plant extracts and its
bioactive compound on A.albopictus and C.quinquefasciatus mosquito species.
• Isolation of mangrove plant crude extract with different solvents using soxhelt apparatus.
• Screening the extract for biological activity against mosquitoes.
• Isolation of the bioactive compound in the extract by chromatography techniques.
• NMR spectral analysis and characterization of isolated compound(s).
• Study the efficacy of purified compound(s) for larvicidal and repellant activities on mosquitoes.
• Volunteer research training at New York University, New York, USA (to learn advanced
molecular biology laboratory techniques)(August 2015 – December 2015)

2. Thameem Azarudeen – Page 2 of 4
Ph.D. PROPOSAL DETAILS
Mosquitoes are well known for their public health importance, Since they act as vectors of many
tropical and sub-tropical diseases such as malaria, dengue, chikungunya, lymphatic filariasis, yellow fever,
and Japanese encephalitis. Aedes albopictus and culex quinquefaciatus (Diptera:Culicidae) are the major
urban vectors of dengue and lymphatic filariasis respectively in India. Controlling of these vectors is being
achieved for a long time by using synthetic chemicals. But they chemicals may cause pollution problem
and help to develop resistance in mosquito species. Emergence of resistance among mosquitoes is a
recent problem. Therefore safe and ecofriendly agents from biological origins are the need of hour.
Biologically active plants extracts have been well documented for evolving an ecologically sound and
environmentally acceptable mosquito control program. A number of such plant products have been used
for insect control for the time immemorial. About 2000 species of terrestrial plants have been reported for
their insecticidal properties. More than 400 terrestrial plants have been screened for their mosquito
larvicidal as per the records. The plant products like Azadirachtin, Pyrethrins, Nicotines, Rotenones are
being used as larvicidae or repellent for pest control. However no detailed studies on effect of plant
derived bioactive compounds on physiological and reproductive activities of mosquito have been
performed. Therefore, the present study is aimed to investigate the effect of mangrove plants extract and
its bioactive compound on larvicidal , pubicidal and repellant activities of mosquitoes Aedes albopictus
and culex quinquefaciatus.
M. PHIL. (MASTER OF PHILOSOPHY) IN ZOOLOGY – (2011- 2013):
BHARATHIDASAN UNIVERSITY, JAMAL MOHAMED COLLEGE, DEPT. OF. ZOOLOGY, TRICHY,
TAMILNADU.
Project Work: “Determination of biochemical adulterations and radionucleotide concentration in different
brands of milk consumed in Tiruchirappalli” - Under the Guidance of Dr. H.E. Syed Mohamed, Asst.
Professor, Jamal Mohamed College, Trichy.
• Bacteriological examination of milk samples.
• Chemical contamination tested using milk adulteration kit from NICE chemicals, INDIA.
• Analysis of Iodine and Potassium isotopes concentration using Gamma-ray spectroscopy (NaI
(TI) Gamma Detector, Spectroscopy Amplifier (with NETS-3MU).
M.SC. IN ZOOLOGY – (2009- 2011):

3. Thameem Azarudeen – Page 3 of 4
BHARATHIDASAN UNIVERSITY, KHADIR MOHAIDEEN COLLEGE, DEPT. OF. ZOOLOGY,
ADIRAMPATTINAM, TAMILNADU.
Project work: “Effect of pesticide (Rogor) on the biogeochemical constituents of Channa punctatus (fish)
habitat”, under the Guidance of Dr. O. Sathik, Asst Professor, Bharathidasan University Khadir
Mohaideen College, Adirampattinam, Tamilnadu.
• Studied the level of pesticide contamination in the habitat and in fish biological samples.
• Quantitative estimation of total protein, glycogen and lipids (Bragdon colorimetric method)
B.SC. IN ZOOLOGY – (2009):
MADURAI KAMARAJ UNIVERSITY, SBK COLLEGE, ARUPPUKOTTAI, TAMILNADU.
Passed with first class marks.
RESEARCH RELATED SKILLS
• Molecular Biology - Experience in PCR, Gel electrophoresis, SDS-PAGE, Western Blot.
Completed certified training course in disease diagnosis using Enzyme Linked Immunosorbent
Assay (ELISA) (Dipstick ELISA, Dot ELISA).
• Cell culture - Fair working knowledge with mammalian cell culture.
• Biochemistry - Protein, DNA, RNA estimation. Preparation of Buffers, pH measurements and
pKa value determination. Experience in using affinity/ion exchange chromatographic techniques.
• Biochemical assays - carbohydrate, glucose estimation, amylase and nitrate reductase tests.
• Microbiology - Sterilization techniques, preparation of culture media, culturing of
microorganisms, Gram staining of microorganisms.
• Histology - Tissue sectioning using microtome (paraffin embedded), staining and analysis of tissue
sections.
• Computer Software - Diploma in computer application-Grade “A”. Proficient in MS Windows,
MSOffice (Word, Excel, PowerPoint) and C Language (C++, HTML).
CONFERENCE PRESENTATIONS
• Research synopsis presented in International Conference on Vector Borne Diseases – Combat
and Control, Justice Basheer Ahmed syed college for women, Chennai, Tamilnadu, India (2014).
• Participated in National Workshop “Analytical Methods And Tools in Biological Research,
Annamalai University, Annamalainagar, Chidhambaram, Tamilnadu, India (2014).
• Participated in “Global Warming on Biodiversity of Insects, Management and Conservation
Strategies. (GW –BIMC), Bharathiyar University, Coimbatore, Tamilnadu, India (2013).
• Paper presented in National Conference On Current Challenges in Environment-Supported by
Association of Environmental analytical Chemistry of India (AEACI), Jamal Mohamed College,
Trichy, Tamilnadu, India (2013).
• Paper Presented in National seminar on Emerging Trends And Challenges In Aquatic
Biotechnology”, Khadir Mohaideen College, Adirampattinam, Tamilnadu, India (2011).
• Paper Presented in “Future Prospective of natural products and food as medicine”-
(CSIR&ICMR), Rajah Serfoji Govt. College, Thanjavur, Tamilnadu, India (2011).
• Attended “National Seminar on Health Care & Management” seminar, Rajah Serfoji Govt.
College, Thanjavur, Tamilnadu, India (2011).

4. Thameem Azarudeen – Page 4 of 4
• Participated in “Science EXPO-2010”, Khadir Mohaideen College, Adirampattinam, Tamilnadu,
India (2010).
• Participated in UGC Sponsored national level seminar on “Animal Food Processing”, V.V.
Vanniaperumal College for Women, Virudhunagar, Tamilnadu, India (2008).
TEACHING EXPERIENCE
• Part time Teaching Assistant, as part of PhD course work at Khadir Mohaideen College.
• Handled zoology (both theory and practical) course for bachelor’s and master’s degree students.
EXTRA-CURRICULAR ACTIVITIES
• Participated in NSS (National Service Scheme) & Camping (2009-2011) Bharathidasan
University.
• Participated in 12 km Marathon Bharathidasan University.(2010).
• NCC (National Cadet Corps) “B” Certificate 2006-2009.
• Attended Army attachment camp with 9th Battalion the Sikh Light Infantry, Bangalore. Ranked
as a SGT (Sergeant)-2008
• Active group member in “ RED RIBBON CLUB”(RRC)-2008
• Participation in “SATHYASODHANI”-SAP CHARITABLE TRUST, Rajapalayam.(2007)
• Regular Blood donor in Blood center (from 2007).
• Winner at Football and Volley ball in Annual day of college.(2006-2009).
• Zone winner, District winner and Divisional runner and in “Throw Ball”(2005).
• NSS School 10 days special camping (2005)
• Kabadi game winner in ,Annual day sports.(2004)
DECLARATION:
I do here by declare that all statements made in the above application are true, complete and found
correct to the best of my knowledge and belief.
Place: ARUPPUKOTTAI Yours faithfully
R. Thameem Azarudeen
REFERENCE:
Dr. A. Amsath,
Associate Professor,
Department of Zoology,
Khadir Mohaideen College,
Adirampattinam, Tamilnadu.
Cell: 952458297
e-mail:aamsath@gmail.com
Dr. Ayesha Mariam,
Professor and Head
Department of Physics,
Khadir Mohaideen College,
Adirampattinam, Tamilnadu.
Cell: 9486738806
e-mail:ashammakmc@yahoo.com
Dr. H. E. Syed Mohamed,
Assistant Professor,
Department of Zoology,
Jamal Mohamed College,
Trichy, Tamilnadu
Cell: 9790112105
e-mail:syed_md@yahoo.com
HANDLED TECHNIQUES:

5. Thameem Azarudeen – Page 5 of 4
Wet Lab Experience :
Biochemistry:
1. preparation of Buffers, pH Measurements and pKa value determination.
2. Qualitative determination of carbohydrates.
i. Molischs test
ii. Anthrone test
iii. Iodine test
iv. Barford’s test.
v. Seliwanoffs test
vi. Benedicts test
vii. Picric acid test.
viii. Mucic Acid test
ix. Bials test.
x. Fehlings test.
3. Quantitative Estimation of Carbohydrate (Anthrone method)
4. Determination of protein concentration (Lowry’s method)
5. Determination of protein concentration (Brad fords method)
6. Quantitative estimation of Amino acids (Ninhydrin method)
7. Paper chromatography for amino acids
8. Estimation of DNA by colorimetric method.
9. Estimation of RNA by colorimetric method.
10. Estimation of Cholesterol by colorimetric method.
11. Salivary Amylase Activity
12. Nitrate Reductase Activity
13. Activity of phosphatase.
14. Immobilisation of Enzyme Alkaline phospate.
15. Native gel Electrophoresis of protein
Microbiology
1. Sterilization techniques
2. Preparation of culture media
3. Culturing of Microorganisms.
4. Staining Techniques
i. Simple staining
ii. Gram staining
iii. Spore staining
iv. Capsular staining
5. Biochemical tests:
i. Indole production test
ii. Methyl Red test,
iii. Voges-Proskauer test,
iv. Citrate utilization test
v. Carbohydrate fermentation test
vi. Triple sugar iron agar test,
vii. Starch hydrolysis
viii. Gelatin hydrolysis
ix. Casin hydrolysis
x. Catalase test,
xi. Urease test,
xii. Oxidase test,

6. Thameem Azarudeen – Page 6 of 4
xiii. Antimicrobial Susceptibility test (Kirby –Baur Method)
6. Qualification of microorganisms by (Turbidity method)
7. Environmental sample Analysis- Water, soil.
8. Isolation of Bacteria and Fungi from soil.
9. Bacteriological examination of milk sample
10. Preservation of bacterial cultures
Histology
● Section the tissues in microtome ( Paraffin embedding)
● Staining the sectioned tissue.
● Analyze the stained sections.
INSTRUMENTS HANDLED
11. Laminar flow hood CLAS., Atlanta, USA.
12. Autoclave Asian Engineering Pvt Ltd, India
13. Deep freezer (-70) Thermo forma, U.S.A.
14. Deionized water Milli-Q, Millipore Corporation, USA.
15. Electroporator BIORAD GENE PULSER II, USA.
16. Micro centrifuge KUBOTA, USA.
17. Gel electrophoresis system American Pharmacia biotech, USA
18. Gel documentation system SYNGENE, GERMANY
19. Ice flakers Ice matic F160 Electronic, India
20. Incubator Yorco scientific industry, India.
21. PCR thermal cycler MJ research PTC 200., Germany.
22. PH meter Digisun Electronics., India.
23. Tabletop Centrifuge Eppendroff., Germany.
24. UV transilluminator Fotodyne., USA.
25. UV-Vis spectrometer Shimadzu., Japan
26. Weighing balance Afcoset FX 300 and FX 1200., India.

7. Thameem Azarudeen – Page 6 of 4
xiii. Antimicrobial Susceptibility test (Kirby –Baur Method)
6. Qualification of microorganisms by (Turbidity method)
7. Environmental sample Analysis- Water, soil.
8. Isolation of Bacteria and Fungi from soil.
9. Bacteriological examination of milk sample
10. Preservation of bacterial cultures
Histology
● Section the tissues in microtome ( Paraffin embedding)
● Staining the sectioned tissue.
● Analyze the stained sections.
INSTRUMENTS HANDLED
11. Laminar flow hood CLAS., Atlanta, USA.
12. Autoclave Asian Engineering Pvt Ltd, India
13. Deep freezer (-70) Thermo forma, U.S.A.
14. Deionized water Milli-Q, Millipore Corporation, USA.
15. Electroporator BIORAD GENE PULSER II, USA.
16. Micro centrifuge KUBOTA, USA.
17. Gel electrophoresis system American Pharmacia biotech, USA
18. Gel documentation system SYNGENE, GERMANY
19. Ice flakers Ice matic F160 Electronic, India
20. Incubator Yorco scientific industry, India.
21. PCR thermal cycler MJ research PTC 200., Germany.
22. PH meter Digisun Electronics., India.
23. Tabletop Centrifuge Eppendroff., Germany.
24. UV transilluminator Fotodyne., USA.
25. UV-Vis spectrometer Shimadzu., Japan
26. Weighing balance Afcoset FX 300 and FX 1200., India.