Truck platooning has the potential to lower variable costs for road haulage companies through reduced labor and fuel costs. Simulations showed savings of up to 5% for large companies with over 200 weekly truck missions through natural overlap in routing and minimal impact on route choice planning. Smaller companies could achieve savings up to 2% through flexible time windows and cooperation, but with a significant impact on route planning. Third party platooning providers represent an opportunity. Successful implementation will require stakeholder cooperation, pilot projects with large companies, and leveraging synergies to benefit smaller companies.