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COMMERCIALISATION OF
NATURAL MEDICINAL
SUBSTANCES
By L. T. M. Muungo
Why Commercialisation?
• Increased for domestic needs
• Research and Development process
• Profit creation and gains
• Medical use value additions
• etc
ENVIRONMENTAL PRODUCTION CONDITIONS
• Plant growth and development and often the
nature and quantity of secondary metabolites
are affected by the following:
➢Temperature,
➢Rainfall,
➢Aspect length of day that includes quality of light ,
➢Type of soil and
➢Altitude
• Studies have shown that cannabis grown
in England are reach in CBD (cannabidiol)
and devoid of THC (tetrahydrocannabinol)
TEMPERATURE
• Temperature is a major factor controlling the
development and metabolism of plants
• Each species has become adapted to its own
natural environment
• Plants are able to exist in a considerable range
of temperature
• Many tropical and subtropical plants will grow in
temperate regions during summer months, but
lack frost resistance to withstand winter
• The highest temperatures are experienced
near the equator, but as the temperatures
fall about 1°c for every 200m of elevation,
it is possible in say Jamaica to have a
tropical climate on the coast and become
temperate in the mountains
• The annual variations in temperature for
Jamaica can be just as important as the
temperature in the hottest month in the
year as from the cooler part of the same
year
• Formation of volatile oils in Hyoscyamus
muticus is affected by temperature
variation
• Logically, hot days may lead to excess
physical loss of volatile oils
• This would mean each plant will have the
mean optimum temperature for production
of volatile oil.
RAIN FALL
• The important effects of rainfall on vegetation
must be considered in relation to the following:
➢ annual rainfall pattern,
➢ its distribution pattern through out the year,
➢ its effect on humidity and
➢ its effect coupled with the water holding
properties of the soil
• Variable results have been reported for the
production of volatile oils under different
conditions of rainfall and the development
of glandular hairs( trichomes)
• Continuous rainfall may lead to excess
loss of water soluble substances from the
leaves and roots by leaching, this is
particularly common in alkaloids and
glycosides even volatile oils
• This could account for low production of
the active constituents in wet seasons
from plants
Length of the day and Radiation Characteristics
• Plants vary much in both the amount and
intensity of light which they require
• Research has shown that light is a factor
which helps to determine the amount of
glycosides or alkaloids produced
• With Cinchona ledgeriana, full sunshine
gives higher content of alkaloids than that
produced in the shade
• During the summer when the days tend to be
longer, production for Cinchona ledgeriana could
be better than the winter days
• Experiment indicate that with Datura stramonium
vartatula, long exposure to intense light brought
a sharp increase in hyoscine content at the time
of flowering
• Irradiation of intact plants of Cantharatus roseus
with ultraviolet light in the range of 290-380 nm
(peak 370 nm) stimulates the synthesis of
dimeric alkaloids probably by inducing
catharathine oxidation as a trigger reaction
• It has been shown that on long day condition
peppermint leaves contain methone, menthol,
and traces of menthofuran
• Plants grown under short day conditions contain
menthofuran as a major component of the
volatile oil
• Long photo period for young leaves activates the
reduction pathway with conversion of menthone
to menthol
• Presence or absence of light together with
wavelength range, have a marked effect on the
secondary metabolite production of some plants
in tissue culture
Altitude
• The coconut palm needs a marinetime
climate and the sugar cane is a lowland
plant
➢Tea - 1000-20000m,
➢cocoa - 100-200m,
➢coffee - 800-1800m
➢Tragacanth and Cinchona require elevated
growing land
➢Cinchona succirubra will grow in low land
but produces no alkaloids
• The bitter constituent of Gentiana lutea
increase with altitude
• alkaloid of Lobelia inflata will decrease and
so will the oil content of peppermint at
certain altitude
• Pyrethrum gives the best yields of
flowering head and pyrethrins at high
altitudes or near the equator
Atmospheric composition
• Digitalis lanata grown in
greenhouses with carbon dioxide
–enriched atmosphere (1000
p.p.m.CO2 during the whole of the
growth period produce 3.5 times
more the amount of digoxin, that
normally would be produced
Cultivated and wild plants
• Certain plants are obtained exclusively from
cultivated plants
• These include Indian hemp (cannabis sativa),
ginger, peppermint, spearmint, cinnamon,
opium, cinchona
• In some cases both wild and cultivated are used
• Owing to the demand and sparse distribution or
inaccessibility ,difficulty in collection, cultivation
becomes essential
• Indian hemp and opium are subjected to
legal control
• In many cases cultivation is advisable
because of the improved quality of the
drug with which it is possible to produce
• The improvement may be due to the
following:
• The power to confine collections to species
varieties or hybrids which have the desired
character e.g. cinchona,cinammon
• The better development of the plants owing to
improved conditions of the soil, pruning ,and
control of insect pests , fungi
• The better facilities for treatment after collection
e.g. drying at correct temperatures ,and peeling
of ginger
• For success in cultivation it is necessary to
study the conditions under which the plant
flourishes in the wild state and reproduce
these conditions or improve on them
• Small changes in ecology can affect plant
products
SOIL
• Different plant species vary enormously in
their soil and nutritive requirements
• Three basic characteristics of soil are the:
➢physical,
➢chemical and
➢microbiological properties
• Variation in particular size results in
different soils ranging from clay, via sand,
to gravel
• Particle size is one factor influencing water
holding capacity , and some plants e.g.
Althaea officinalis which produce mucilage
as a water retaining material contain less
mucilage when grown on soil with high
moisture content.
• In moist regions such as Western Europe,
clay soil absorb water beyond their
absolute capacity
• Their high moisture content make them
cold i.e. they heat up slowly
• In drier regions such as the
Mediterranean's soils have the capacity to
absorb and retain moisture
• The basic soil type can be modified by the
presence of humus, organic fertilizers,
chalk lime etc.
• Fine soil rich in humus and having a
permeable substratum posses a degree of
humidity which is favorable for plants
• Sandy soil are suitable for xerophilous
(plant adapted for life with a limited supply of water) plants
• Soils containing much humus and little
lime are inclined to be more acidic while
those with abundant lime are alkaline
• All plants require calcium for their normal
nutrition
• The effect of nitrogen-fertilizers as soil
nutrients will increase the size of the plant
and the amount of total alkaloids and
glycosides produced
Medicinal Plants Growth
Propagation
Growth Propagation from seeds
• To ensure success the seed must be collected
when perfectly ripe
• If not planted immediately they must be properly
stored in a cool dry place and must not be kiln
(artificial) dried
• Some seeds like cinnamon cocoa and nutmeg
lose their power to germinate if allowed to dry or
if stored for quite short periods
• In some, long storage decrease the percentage
of germination e.g. some cereals and nuts
• Seed can be sown at different time of the year
• Some seeds are sown in spring while others can
be immediate as fresh seed e.g. colchicum
autumnale. If dried just for a few days only 5%
will germinate in a year
• Seeds slow to germinate can be soaked in water
or a solution of Geberrilic acid solution for 48 hrs
before sowing to increase the chances of
germination
Growth Propagation by vegetative
means
1. By the development of bulbs
squill, corms, e.g. colchicum,
tubers e.g. jalap or
rhizome e.g. ginger
2. By division, a term usually applied to the
separation of a plant which has a number
of aerial stems or buds into separate parts
each having roots and a growing point this
method may be used for Althaea, Gentiana
3. By runners or offsets e.g. chamomile and
mints
4. By suckers or stolon e.g. liquorice and
valeriana
5. By cuttings or portions of the plant
severed from the plant by use of rooting
hormones
- Cuttings may be employed for the
propagation of mints, lavender,
rosemary
6. By layers: A layer is a branch or shoot which is
induced to develop roots before it is completely
severed from the parent plant
- This is done by partly interrupting the food
supply by means of a cut and embedding the
part
7. By grafting and budding: Grafting is an
operation which two cut surfaces usually
of different but closely related plants are
placed so as to unite and grow together
• The rooted plant is called the stock and
the portion cut off is the scion or graft e.g.
Cinchona
• Budding consist of the introduction of a
piece of bark bearing a bud into a suitable
cavity or T-shaped slit made in the bark of
the stock
• Budding is used in citrus spp
8. By fermentation : This process applies to
the production of moulds and bacteria and
is used for the manufacture of antibiotics
and some vitamins
9.By inoculation : Specific to ergot whereby
spores are of the fungus are artificially
cultured and injected into the rye by
special machines
10.By cell culture: The same example as for
ergot spores.
Propagation by means of Chemical
Growth Regulators
• The growth and development of plants
may be regulated by a number of
intentional added chemical substances
such as:
➢Auxins
➢Gibberellins
➢Cytokinins
➢Abscisic acid
➢Ethelyne
• These substances occur naturally in all
plants
• They are specific in their action and are
very active in very low concentrations
• They regulate cell enlargement, cell
division, cell differentiation,
organogenesis, senescence and
dormancy
• Other hormones are concerned with
flowering formation and reproduction
• The essential role of these hormones is
illustrated in cell and tissue culturing
Auxins
• These are growth-promoting substances
which are divided into the following:
➢Auxin-a
➢Auxin –b
• They are obtained from human urine and
cereal products
• They have similar properties to indole 3-
acetic acid (IAA)
• Auxins are found in actively growing
tissues and is derived in the plants from
tryptophan
• Effects of auxins are cell elongation giving
an increase in stem length of root growth
adventitious roots production and fruit
setting in the absence of pollination
• Synthetic auxins include:
➢Indole-3-butyric acid (I-3-BA)
➢Naphthalene-1-acetic acid (NAA)
➢2,4-dichlorophenoxyacetic acid (2,4-D)
USES FOR AUXINS
1. In low concentrations to accelerate the
rooting of woody and herbaceous cuttings
2. (Placed for 24hrs in a 1: 500,000 solution
of NAA, cuttings develop roots)
3. In higher concentrations to act as
selective herbicides or weed-killers
4. Seedlings of Mentha piperitta when
treated with derivatives of NAA increased
the yield 30-50% of oil which contained
4.5%-9% menthol
5. Auxins on Datura stramoniun showed an
increase in production of trichomes,
production of smooth fruits but there was
no increase production of the tropane
alkaloids
Gibberellins
• Discovered by Japanese worker in
connection while working in the fields of
rice.
• This was during the the disease of rice
fields
• The effect on the plant is excessive
elongation the plant to fail to support itself
• The causative organism of the disease is
Gibberella Fugikuroi
• Gibberellins are distinguished as
GA1,GA2,GA3…….
• GA3 commonly known as gibberellic acid
and is produced commercially by fungal
cultivation
• Gibberellins are synthesized in leaves and
they accumulate in large quantities in the
immature seeds and fruits
• Application of gibberellins to short –node
plants produce rosettes of leaves e.g.
Digitalis lanata and Hyocymus niger
• Gibberellins initiate the synthesis of
hydrolytic and proteolytic enzymes upon
which seeds germinate
• The growth effect of gibberellins arises by
cell elongation in the sub-apical meristem
region where young internodes are
developing
• As with auxins, gibberellins occur in plants
in deactivated forms
Effects on Alkaloids
• GA treatment with the seeds assist in
obtaining a uniformity germination and
total emergence
• The plants elongation gives 2-3 folds
increase in height, rapid onset of flowers
and decrease in dry weight
• Cantharatus recorded a lowering of
alkaloid content
• Datura treated with GA showed elongation
of the stems and reduced alkaloid content
EFFECTS ON VOLATILE OIL
• GA treatment of volatile oil containing
plants showed changes in morphological
characters
• Oil of peppermint gave an increased yield
by 40%
Cytokinins
• Cytokinins have specific effects on cell
division (cytokinesis)
• Cytokinins also regulate the pattern and
the frequency of organ production as well
as position and shape of the plant stem
• Cytokinins promote formation of
adventitious buds and shoots from
undifferentiated cells in tissue culture
• They have an inhibitory effects on
senescence
Abscisic acid (ABA)
• These are generally referred to as Growth
Inhibitors
• Natural growth inhibitors are present in
plants and affects bud opening, seed
germination and development of dormancy
• One such substance is Abscisic acid
(ABA)
• These act as inhibitors to shoot elongation
• Sprayed on plants showed reduced height
Ethylene
• Ethylene induces growth Reponses in
plants
• Ethylene is synthesized in plants from S-
adenosylmethonium
• However, it has been found to inhibit the
growth of potato shoots
Medicinal Plants Harvesting
COLLECTION
• Drugs may be collected from the wild or
cultivated plants
• It is known that the active constituents of
medicinal plants are affected by many
factors and may vary during the course of
plant growth.
• This also requires skilled and unskilled
labour
Collection of medicinal plants
• The season at which the drug is collected is as
important as the environment of growth - Proper
time of collection is very important to obtain a
drug of a good quality.
• The amount of the active constituents is not
constant through out the year
• The age of the plant is also important as it would
depend on the time of collection
• The age governs the total quantity of the
active constituent produced but also the
relative proportion of the components of
the active mixture
• Composition of the secondary metabolites
varies throughout the day and night
• Leaves are collected when the flowers
begin to open
• Under ground organs are collected as the
aerial part die down e.g. nuts
• Leaves flowers and fruits should not be
collected when covered with dew or rain
• Any plant attacked by mould or insects
should not be collected
• It is almost impossible to collect one part
of the plant without collecting other parts
• A certain percentage of stalks parts are allowed
according the official monographs as “foreign
matter” as defined by the BP or EP
• Special machines are used to collect ergot and
lavender
• Reaping machines are sometimes used as
compared to manual or human labour
• Barks are usually collected after a period of
damp weather which makes it easier for the bark
to separate from the wood
• Gums and gum resins are collected in dry
weather
• Care must be taken when collecting to
reduce debris
• Underground organs must be free from
soil and must be dug in dry weather e.g.
potate
• Certain under ground organs must be
washed and others are peeled e.g.
carotine
Factors affecting collection
1. Time of the year:
The plant may contain a substance in winter that is not
present in summer, or its amount varies markedly e.g.
Rhubarb contains no anthraquinone in winter, instead
it contains anthranols, which in summer, are oxidized to
anthraquinones.
Colchicum corm is free from bitterness and is devoid of
the alkaloid colchicine in autumn, hence is used in
Austria as a food, instead of potatoes. Bitterness starts
to appear in spring and early summer when it is used
as a drug.
2- Time of the day:
Some drugs, like Digitalis, contain different
amounts of active constituents at different
times of the day. Being highest in the
afternoon.
3- Stage of maturity and age:
• The value and content of active
constituents of many drugs depends on
the stage of maturity and age.
• Conium fruits contain coniin when fruits
are mature and unripe.
• Santonica flowers are rich in santonin,
when unexpanded, when it starts to open,
the santonin content decreases.
Drying of crude drugs
Reasons for drying:
1. To help in their preservation.
2. To fix their constituents, by preventing
reactions that may occur in presence of water.
3. To prevent the growth of micro-organisms such
as bacteria and fungi.
4. To facilitate their grinding.
5. To reduce their size and weight.
6. Insufficient drying favors spoilage by micro-
organisms and makes it possible for enzymatic
destruction.
• If enzymes’ action is to be encouraged slow
drying at moderate temperatures is necessary
e.g. cocoa
• If enzymatic action is not desired drying should
take place as soon as possible after collection
• Drugs containing volatile oil are liable to lose
their aroma if not dried or if the oil is not distilled
from them immediately
• All moist drugs are likely to develop mould if left
long in a dumpy environment
• For this reason drying apparatus and stills
should be situated as near to the growing
plants as possible
• The duration of drying varies from a few
hours to weeks
• Open air drying is used for cloves,
cinnamon
• For drying in shades drugs may be
suspended in bundles
• Trays are also used that are made of wire
mesh
• Drying by artificial heat is used where
rapid drying is desired or where humidity is
high
• Continuous belts are used for drying large
crops such as digitalis
• Heat may also be applied as open fires, by
use of stoves and hot water pipes
• In all drying sheds the space between the
trays should be 15cm apart
• Rapid drying of flowers helps them retain
their colour and aromatic drugs also retain
there aroma
• Leaves, herbs and flowers may be dried at
20-40c
• Barks and roots slightly higher than that of
leaves as above, at 30-65c
Methods of drying
Drying is carried out either by natural or artificial
methods.
1- Natural drying: this is accomplished by natural
air in sun or shade.
2- Artificial drying: this is a rapid method done at
well-controlled temperature and is accomplished
by:
• direct fire.
• Use of heated stones.
• Use of stoves.
• Lyophilization (Freeze drying):
Frozen material is placed in an evacuated apparatus which has a
cold surface maintained at -60 to -80 °C. Water vapour from the
frozen material passes rapidly to the cold surface.
It is used for drying heat-sensitive substances e.g. antibiotics and
proteins.
• Chemical drying using desiccators
• An absolutely dried drug is that completely freed from water,
when exposed to air it absorbs 8-10% of moisture and is called
air-dry drug.
Stabilization
• On long storage, enzymatic reactions will
slowly destroy the constituents, because the
last traces of water can never be removed.
• In order to avoid this degradation, the
enzymes should be destroyed before drying,
a process usually called stabilization.
• The most common method being brief
exposure (a few minutes only) of the plant
material to ethanol vapor under pressure (0.5
atm).
Fermentation
• Enzymatic transformation of the original plant
constituents is sometimes desirable. e.g. Vanilla pods
• The fresh material is placed in thick layers,
sometimes covered and often exposed to raised
temperatures (30-40 °C) and humidity, so as to
accelerate the enzymatic processes.
• The fermented product must be dried afterwards to
prevent attack by microorganisms, e.g. moulds.
Preservation and protection of
crude drugs
Storage represents the last stage of preparing crude drugs.
Drugs usually deteriorate along the time of storage, except in few cases
e.g. Cascara and Frangula should not be used except after certain period
of storage.
Certain drugs such as Nux vomica are hardly affected by storage.
Generally, changes that take place during storage of crude drugs are
objectionable, e.g. drugs containing volatile oils gradually lose their
aroma.
Improper methods of storing and inadequate protection during storage can
cause a pronounced deterioration.
There are two principal reasons for deterioration:
Physiochemical: moisture, heat, air and light.
Biological: fungi, bacteria, insects and rodents.
Physicochemical factors
1. Moisture: moisture sometimes affects drugs adversely
through activating the enzymes (as in cardiac
glycosides).
2. Heat: rise of temperature up to 45 activates the
enzymes causing decomposition of active constituents.
Volatile oil containing drugs are also affected by higher
temperatures, their content decreases.
3. Air: oxygen of air oxidizes certain constituents of crude
drugs, e.g. linseed and lemon oil, it causes rancidity of
fixed oils and resinification of volatile oils.
4. Light: it affects drugs, especially those having marked
colours.e.g. yellow colour of Rhubarb changes to
reddish tint, white coloured corollas turn brown.
Biological factors
1. Bacteria: cotton fibres are rendered brittle by bacterial attack
which makes the cotton wool objectionable and dusty.
2. Moulds: the mycelium of delicate hyphae produces an
unpleasant mass of clinging particles in powdered drugs.
3. Insects: they seem to attack all drugs but have preferences to
certain drugs as ginger, belladonna, kola, liquorice - Insects
which infest vegetable drugs include beetles, mites and moths.
They render drugs porous and powdery.
4. Rodents: they cause much spoilage of crude drugs during
storage, especially if wrapped in paper, cloth or put in
cardboard or wooden containers. The presence of rodent's
filth, excreta, hairs causes rejection of the drug.
Methods for controlling insects
1. Heat treatment: it is the simplest method and
is done by exposing the drug to a temperature
of 60-65. it is effective especially for insect
eggs which are not affected by insecticides.
2. Fumigation: this is done by volatile
insecticidal agents in closed areas e.g. CCL4,
CS2, CN. Most fumigants do not kill eggs of
insects. It is advisable to repeat fumigation at
intervals to obtain better results.
3. Liming: liming of certain drugs as ginger,
nutmeg to protect against insect attack provides
only partial protection.
4. Low temperature storage: this method is
preferred to fumigants and liming. Adult insects,
pupae, larvae and eggs are sometimes killed by
very low temperatures.
5. Exposure to alternate periods of low and
high temperatures: frequently is more effective
for killing insects than a prolonged period of low
temperature exposure.
NATURAL PRODUCTS PROCESSING CONDITIONS
Grinding of crude drugs
• Regardless of whether the crude drug is to be used
for isolation of a pure compound or for manufacture
of a simple preparation, the first operation that must
be performed is grinding of the plant material to a
powder of suitable particle size.
• It is important that the particles are of as uniform size
as possible.
• Excessive dust can clog percolators and result in a
turbid extract which is hard to clarify.
• Large particles take a longer time for complete
extraction than small ones.
• Large differences in particle size thus slow down the
extraction process.
Types of machines used for grinding crude drugs:
1. Hammer mill; a common type for grinding crude
drugs.
2. Knife mill; is useful for production of low-dust
powders of leaves, barks and roots for subsequent
percolation or maceration.
3. Tooth mill; is used for production of very fine
powders.
Notes:
• Grinding produces a certain amount of heat
which must be observed when grinding crude
drugs containing heat-sensitive compounds.
• Mills cooled with liquid nitrogen are available
for such purposes.
• Cold grinding is also preferable for crude
drugs containing volatile oils.
• Following grinding, the material must be
sifted to ensure the proper particle size.
Extracts
• Extracts can be defined as preparations of crude
drugs which contain all the constituents which are
soluble in the solvent used in making the extract.
• In dry extracts all solvent has been removed.
• Soft extracts and fluid extracts are prepared with
mixtures of water and ethanol as solvent.
• Tinctures are prepared by extraction of the crude
drug with five to ten parts of ethanol of varying
concentration, without concentration of the final
product.
• Plant constituents are usually contained inside the
cells. Therefore, The solvent used for extraction must
diffuse into the cell to dissolve the desired
compounds whereupon the solution must pass the
cell wall in the opposite direction and mix with the
surrounding liquid.
• An equilibrium is established between the solute
inside the cells and the solvent surrounding the
fragmented plant tissues.
The speed with which this equilibrium is
established depends on:
1. Temperature
2. pH
3. Particle size
4. The movement of the solvent
Choice of solvent
• The ideal solvent for a certain pharmacologically
active constituent should:
1. Be highly selective for the compound to be extracted.
2. Have a high capacity for extraction in terms of
coefficient of saturation of the compound in the
medium.
3. Not react with the extracted compound or with other
compounds in the plant material.
4. Have a low price.
5. Be harmless to man and to the environment.
6. Be completely volatile.
• Aliphatic alcohols with up to three carbon atoms, or
mixtures of the alcohols with water, are the solvents
with the greatest extractive power for almost all
natural substances of low molecular weight like
alkaloids, saponins and flavonoids.
• According to the pharmacopoeias, ethyl alcohol is
the solvent of choice for obtaining classic extracts
such as tinctures and fluid, soft and dry extracts.
• The ethanol is usually mixed with water:
1- to induce swelling of the plant particles
2- to increase the porosity of the cell walls which facilitates the
diffusion of extracted substances from inside the cells to the
surrounding solvent.
• For extraction of barks, roots, woody parts and seeds the
ideal alcohol/water ratio is about 7:3 or 8:2.
• For leaves or aerial green parts the ratio 1:1 is usually
preferred in order to avoid extraction of chlorophyll.
Extraction Procedures
There are many procedures for obtaining
extracts
1- Infusion
2- Maceration
3 Percolation
4- Digestion
5- Decoction
6- Continuous hot extraction
7- Solvent-solvent precipitation
8- Liquid-liquid extraction
9- Distillation 10- Specific procedures
STORAGE
• Long storage for herbal drugs is not
recommended
• Indian hemp deteriorates with long storage
• Drugs can be stored in sacks, cardboard
boxes ,wooden cases and paper bags
which, enables them to reabsorb about 10-
20% surrounding moisture
• These are termed air dry materials with
such moisture percentage content
• Plastic sacks will seal effectively the
contents
• Permissible moisture for starch ,acacia
gum is found in standard International
Pharmacopeias such as EP, BP, USP etc
• A combination of moisture and
temperature on humidity and the
subsequent water condensation when
temperature falls must be considered in
during storage as it can be detrimental to
the substances
• They must be kept in sealed containers
with dehydrating agent
• For large quantities the bottom of the
box may be filled with quicklime and
separated from the drug by perforated
grid or sackings
• Volatile oil should be sealed in well-filled
containers and in cool dark places
The End
Terrestrial Ecosystem
(Biomes)
Terrestrial Ecosystem Response to
Climate Change – This is just a reminder
of the global natural system changes on
global natural medicinal plants
distribution
Terrestrial Ecosystem (Biomes)
Global Change and Effects on
Terrestrial Ecosystem
Introduction
• Temperature, precipitation, latitude and altitude all
determine distribution of major terrestrial ecosystems
(biomes).
• Plants found within the different biomes are influenced
by soil type, water shed conditions and amount of sun.
• Specific combinations of temperatures and
precipitation ensure the survival and thriving of plants
within a given environment (known as Climate Space
and Change).
Terrestrial Ecosystems are an…
• Integral part of global carbon system
• As earlier stated that plants take in and store carbon dioxide from the atmosphere through
photosynthesis
• Below ground microbes decompose organic matter and release organic carbon back into the
atmosphere
Cycle shows how nature’s sources of CO2 are self regulating – that which is released will
be used again – Anthropogenic carbon not part of natures cycle – is in excess
www.bom.gov.au/.../ change/gallery/9.shtml
Forests
• Forests occupy major portions of land mass in different
countries.
– In the U.S. forests occupy 33% of the land mass
while in Africa, natural forestry occupy over 60%
of the land
• Forests have many functions:
– Provides habitat for plants and animals
– Influence amount of and availability of water
runoff
– Provide sites for recreation
– Provide timber for harvesting lumber; wood pulp,
fire wood for fuel
– Total commercial estimated valued in U.S. =
$290 billion and more than twice for Africa
Land Formation
• 18,000 years ago glaciers retreated in the Northern hemisphere
resulting in a rocky and lifeless ecosystem
• Pioneer plants scientifically may have emerged due to the following:
– Lichens – broke down rock
– Decaying lichens mixed with broken rock chips developed
soil (long/slow process)
• Winds blew – soil flew – dispersed into crevices of rocks – provided
nutrients for small plants to grow
• Soon mosses appeared followed by grasses, then larger plants
(shrubs & trees) known as primary succession
Succession and Climax Forests
•Primary succession - How temperate forests first
emerged
•As forests continue to grow – become more diverse
in both plant and animal life
•Simple plants unable to compete – live in the
shade of larger plants
•Saplings unable to develop due to shade - slowly
die out (Birch, Aspen) – gives rise to middle stage
succession
•Shade tolerant plants emerge (Maple, Hemlock)
and dominate forests – now have a climax forest
Secondary Succession
• Secondary succession occurs quicker than primary succession (soils
already there)
– Exception to this – land cleared for development and agriculture
• Temperate forests dominate because of their resistance to fires
• Less resistant trees eventually die off – leaving the evergreens still standing
• Fires promote evergreen growth by busting seed coats open and scattering
them in the soil
• Deciduous forest devastated by fires
• Secondary succession only way new forest will dominate
people.eku.edu/ritchisong/ secondarysuccession
A look back into time…..
Driving forces effecting global
temperature;
•Plate tectonics
•Earths orbital geometry
(eccentricity, obliquity and
precession)
Billions of years ago
Major continental shifts
– earth extremely hot
Underwent cycles of
glaciations
ice sheets existed
at lower latitudes
Paleozoic Era
Interglacial period
plants invade
land
Permian
Major Glaciation
Paleocene
Elevated greenhouse
gases warmed up
planet
Palm trees in Alaska
Crocodiles in the
Arctic
610 – 575 Ma
438 – 408 Ma
292 - 250 Ma
55 – 52 Ma
Antarctic
ice sheets
formed
34 Ma
Pleistocene
Ice Age
1 Ma
1.8 Ma –
18 Kya
Holocene – cycle of
glaciation and
melting of ice caps
– rising sea levels
18 Kya
Last glacial
period
Changes in Global Climate 65 ma to Present
Asteroid hit Chesapeake led
to major glaciation period
Billions of years ago
Major continental shifts
– earth extremely hot
Underwent cycles of
glaciations
ice sheets existed
at lower latitudes
Paleozoic Era
Interglacial period
plants invade
land
Permian
Major Glaciation
Paleocene
Elevated greenhouse
gases warmed up
planet
Palm trees in Alaska
Crocodiles in the
Arctic
610 – 575 Ma
438 – 408 Ma
292 - 250 Ma
55 – 52 Ma
Antarctic
ice sheets
formed
34 Ma
Pleistocene
Ice Age
1 Ma
1.8 Ma –
10 Kya
Holocene – cycle of
glaciation and
melting of ice caps
– rising sea levels
18 Kya
Last glacial
period
Time Line of Plant Life
Paleozoic era (438 – 408 Ma)
Bryophytes
Cretaceous era (110 Ma)
Angiosperms dominate
Carboniferous (360 – 286 Ma)
Ferns, Gymnosperms
Liverworts
Hornworts
Mosses
taggart.glg.msu.edu/isb200/fland.htm
Jurassic (213 – 144Ma) → Cretaceous (144 – 65 Ma)
570 505 438 408 360 286 245 208 144 66 2 10K
Million years before present
Global Temperature
Paleogeographic Biome
Late Jurassic (150 Ma)
www.geo.arizona.edu/ ~rees/Jurassic.html
Five Main Biomes:
• winter wet (seasonally dry)
• summer wet (subtropical)
• desert
• warm temperature
• cool temperate
▪ Fossils of plant life
used to reconstruct
climate biomes
summerwet
(subtropical)
warm temperature
cool temperature
winter wet
(seasonally dry)
desert
570 505 438 408 360 286 245 208 144 66 2 10K
Million years before present
Prentice, C.I., Guiot, J., Huntley, B., Jolly D. and Cheddadi, R., 1996,
Reconstructing biomes from palaeoecological data:
a general method and its application to European pollen data at 0 and 6 ka.
Climate Dynamics 12:185-194.
Global Distribution of Vegetation
18,000 years ago
conifers
tundra
taiga
grassland
woodland
desert
Prentice, C.I., Guiot, J., Huntley, B., Jolly D. and Cheddadi, R., 1996,
Reconstructing biomes from palaeoecological data:
a general method and its application to European pollen data at 0 and 6 ka.
Climate Dynamics 12:185-194.
Global Distribution of Vegetation 6,000 years ago
taiga
temperate deciduous
woods & scrub
conifers
grassland
desert
tundra
cold deciduous
Prentice, C.I., Guiot, J., Huntley, B., Jolly D. and Cheddadi, R., 1996,
Reconstructing biomes from palaeoecological data:
a general method and its application to European pollen data at 0 and 6 ka.
Climate Dynamics 12:185-194.
Global Distribution of Vegetation - Present
taiga
tundra
temperate deciduous
grassland
cold deciduous
tropical R.F.
warm mix
18 kya
9 kya
Present
Distribution of spruce and oak forests in Northern Hemisphere since the
glacial period 18,000 kya
spruce
spruce
spruce
spruce
spruce
ice sheet
ice
ice
ice
ice
oak
oak
oak
oak
• 18,000 years ago Spruce
trees and oak trees filled
small pockets of habitat –
as climates warmed
Spruce trees migrated
into the Northern
Hemispheres and the
Oak trees expanded in to
Southeastern U.S.,
Western Europe and
Southern Europe
• Shifts in vegetation occur
slowly tree species were
able to successfully
expand into favorable
regions
Shifts in Terrestrial Habitat
18Kya
9Kya
Present
Major Biomes and Their Vegetation
• Tundra – no trees, lichens, grasses and shrubs
• Taiga (or Boreal Forest) – coniferous
evergreens
• Temperate forests – include evergreens
(spruce), deciduous forests (oaks), mixed
forests, and temperate rain forests (sequoias)
• Tropical rain forests – greatest amount of
diversity in vegetation (vines, orchids, palms)
• Grasslands – grasses, prairie clover
• Deserts – cacti, small bushes
Major Terrestrial Biomes
• Geographic distribution of biomes are dependent on temperature,
precipitation, altitude and latitude
• Weather patterns dictate the type of plants that will dominate an
ecosystem
faculty.southwest.tn.edu/. ../ES%20%20we16.jpg
Major Terrestrial Biomes
• Geographic distribution of biomes are dependent on temperature,
precipitation, altitude and latitude
• Weather patterns dictate the type of plants that will dominate an
ecosystem
faculty.southwest.tn.edu/. ../ES%20%20we16.jpg
P
R
E
S
E
N
T
D
A
Y
B
I
O
M
E
S
tundra
taiga
desert
temp.
decd.
forest
taiga
desert
desert
trop.
rain
forest
grassland
temp
rain
forest
scrub
forest
Alaska
Greenland
Ice land
Siberia
Canada
Location:
Regions south of the ice caps of the Arctic
and extending across North America,
Europe and Siberia (high mountain tops)
Yakutsk
Tundra
N. Europe
Average Temperature: -40°C to 18°C
Average Precipitation:
150 to 250 mm of rain per year
Type of vegetation:
Almost no trees due to short growing
season and permafrost; lichens, mosses,
grasses, and shrubs
Yakutsk, Russia
Location:
62.1 N; 129 W
earthobservatory.nasa.gov
Average annual temperature
and precipitation
Temperature:
-40°C to 20°C, average summer temperature
is 10°C
Precipitation:
300 to 900 millimeters of rain per year
Vegetation:
Coniferous-evergreen trees
Location:
Canada, Europe, Asia, and the United States
Other:
Coniferous forest regions have cold, long,
snowy winters, and warm, humid summers;
well-defined seasons, at least four to six frost-
free months
Boreal Forest (Taiga)
Canada Europe Asia
S.W. U.S
Beaverlodge, Alberta, Canada
earthobservatory.nasa.gov
Boreal Forest (Taiga)
Average annual temperature
and precipitation
55oN; 119o W
Average Yearly Temperature:
Between -30°C to 30°C; hot summers, cold
winters; sunlight varies between seasons
Average Yearly Precipitation:
750 to 1,500 mm of rain per year
Vegetation:
Deciduous - Broadleaf trees (oaks, maples,
beeches), shrubs, perennial herbs, and mosses.
Evergreen – (N. America) – Spruce, Hemlock,
Pine and Fir trees
Temperate Rainforests – (CA, OR, & WA) –
made up of Red wood and Sequoias
www.windows.ucar.edu/.../ earth/forest_eco.html
Four types:
1. deciduous forests
2. evergreen forests
3. mixed deciduous and evergreen
4. temperate rain forests
Location:
Eastern United States, Canada,
Europe, China, and Japan
Temperate Forests
Staunton, Virginia, United States
38oN; 79oW
earthobservatory.nasa.gov
Average annual temperature and rainfall
Tropical
Forests
General Characteristics:
Average Temperature: 20°C to 25°C, must remain
warm and frost-free
Average Precipitation: 2,000 to 10,000 millimeters of
rain per year
Average Humidity: Between 77 – 88%
Types of Vegetation: Large trees reaching up to 240
feet, have the most diverse trees than any other
biome: vines, orchids, ferns
Tropical rainforests:
• Cover less than 6% of Earth’s land surface
• Produce more than 40% of Earth’s oxygen
• Contain more than half of all the worlds
plants and animals
• ¼ of all medicines come from rainforest plants
• Scientists believe more than 1400 tropical plants
thought to be potential cures to cancer
3 major geographical areas:
1. America: Amazon river basin
2. Africa: Zaire basin, small area of W. Africa, Eastern portion of Madagascar
3. Asia: West coast of India, Assam, S.E. Asia, New Guinea and Queensland,
Australia
Tropical Rainforest
Amazon river basin Zaire
Madagascar
India S.E. Asia
Queensland
New Guinea
"Rainforests", http://passporttoknowledge.com/rainforest/GEOsystem/Maps/se_asia.html, (3/18/02)
Campa Pita, Belize
15 N latitude
earthobservatory.nasa.gov
Tropical Forest
Average annual temperature and
precipitation
Southeast Asia Tropical Rainforest
Monsoons role
• SE Asia has a tropical wet climate which is influenced by ocean wind
systems originating in the Indian Ocean and China Sea
• 2 monsoon seasons:
– Northeast monsoons (Oct. – Feb) – bring heavy rains to Eastern side of
the islands
– Southwest monsoons (April – Aug) – more powerful of the two seasons
– brings heavy rainfall to the western side of the islands – Eastern side
of islands dry – but windy (due to rain shadow)
• Change in monsoon cycle bring heavy consequences
– Ex. 1992 – 1993 – logging degraded primary foresting making it vulnerable
to fires. A drought brought on by El Nino created devastating fires
destroying 27,000 km2 of acreage.
– In 1998 the same type of thing happened again when El Nino created a
weak monsoon season – destroying many plant and animal species.
Monsoons Seasons
Summer monsoon
India
Indian
Ocean
S. China
Sea
Location: The prairies of the Great
Plains of North America, the pampas of
South America, the veldt of South
Africa, the steppes of Central Eurasia,
and surrounding the deserts in
Australia
Temperature: Dependent on latitude,
yearly range can be between -20°C to
30°C
Precipitation: About 500 to 900 mm of
rain per year
Vegetation: Grasses (prairie clover,
salvia, oats, wheat, barley,
coneflowers)
Other: Found on every continent
except Antarctica
Grasslands
Ingeniera White, Argentina
40oS; 6oW
earthobservatory.nasa.gov
Average annual temperature and rainfall
Location: Found north and south of the
Equator
Temperature: Average of 38°C (day),
average of -3.9°C (night)
Precipitation: About 250 mm of rain per yr
Vegetation: Cacti, small bushes, short
grasses
Other:
Perennials survive for several years by
becoming dormant and flourishing when
water is available. Annuals are referred to
as ephemerals because some can complete
an entire life cycle in weeks.
Deserts
El-Oasr el-Akhdar, Egypt
26oS; 30oE
earthobservatory.nasa.gov
Average yearly temperature and rainfall
So … what are the
predictions?????
Present day
Predicted Distribution
for
est
for
est
grassland
grassland
ari
d
woodl
and
shrub land
shrub land woodl
and
grassland
grassland
Climate change p. 104
• Arid deserts in Southwestern U.S.
will shrink as precipitation
increases
• Savanna/shrub/woodland
systems will replace grasslands in
the Great Plains
• Eastern U.S. – forests will expand
northerly – weather conditions will
become more severe
• Southeastern U.S. – increasing
droughts will bring more fires –
triggering a rapid change from
broadleaf forests to Savannas
Predicted Change in Biomes
Loss of existing habitat that could occur under doubling of CO2
concentration. Shades of red indicate percentage of vegetation
models that predicted a change in biome type.
Distribution of Sugar
Maple in Eastern North
America will change due
to an increase in
temperature and a
decrease in moisture
shifting further north east.
present range
Prediction based on Prediction based on increased
increased temperature temperature and decrease
precipitation
present range
Predictions of Sugar Maple in Eastern North America
overlap
predicted new
growth
predicted new
growth
overlap
Wet western
slope will
shrink and be
replaced by
pine and oak
Eastern slope
will become
drier and shift
to Juniper and
Sagebrush
More Predictions Western Hemlock
and Douglas fir
found on Western
slope
❑ Douglas Fir found in
wet coastal mountains of
CA and OR will shrink in
low lands and be
replaced by Western
pine species which are
more drought tolerant.
❑ Overall Western U.S.
climate is predicted to
shift to favor more
drought tolerant species
of pine
Frequency of forest fires will increase,
reducing total American boreal forest area.
Shifts in Terrestrial
Habitat
• It is predicted that at the
end of this century there
will be large scale shifts in
the global distribution of
vegetation in response to
anthropogenic climate
change.
• With man doubling the
amount of carbon dioxide
entering into the
atmosphere the climate is
changing more rapidly then
plant migration can keep
up.
Potential distribution of the major world
biomes under current climate conditions
Projected distribution of the major world biomes by
simulating the effects of 2xCO2-equivalent concentrations
www.usgcrp.gov/usgcrp/ seminars/960610SM.html
Boreal and Alpine
Vegetation
• Research indicates the greatest
amount of change will occur at
the higher latitudes
• Northern Canada and Alaska are
already experiencing rapid
warming and reduction of ice
cover
• Vegetation existing in these areas
will be replaced with temperate
forest species
• Tundra, Taiga and Temperate
forests will migrate pole ward
• Some plants will face extinction
because habitat will become too
small (ex. Mountain tops of
European Alps)
Predicted changes in Siberian vegetation in
response to doubling of CO2
Climate change
Grasslands and Shrub
Lands
• Grassland will change to deserts or shrub lands
– Exposing greater amounts of soil
– Increasing soil temperature – poor nitrogen content – poor plant
growth
– Barren soil exposed to winds and transported into atmosphere
as dust and trapping IR – leading to more warming
• Models of:
• Climate change
• Plant growth
• Soil – water
Predict shifts in distribution of major North American prairie
grasses over a 40 year period
Those at Risk
• Northern countries (Russia, Sweden, Finland) ½ of
existing terrestrial habitats at risk
• In Mexico, it’s predicted that 2.4% of species will lose
90% of their range and threatened with extinction by the
year 2055
• Population at greatest risk are the rare and isolated
species with fragmented habitats or those surrounded by
water, agriculture or human development
• Polar bears facing extinction by prolonged ice melts in
feeding areas along with decline in seal population
www.sciencedaily.com/.../ 09/050918132252.htm
❑ 35% of worlds existing terrestrial
habitat predicted to be altered
❑ Studies found that deforestation in
different areas of the globe affects
rainfall patterns over a considerable
region
▪ Deforestation in the Amazon
region of South America
(Amazonian) influences rainfall
from Mexico to Texas and in the
Gulf of Mexico
▪ Deforesting lands in Central
Africa affects precipitation in the
upper and lower U.S. Midwest
Phenological
Changes
• Life-cycles of plants and animals have been
affected by global change
• Temperatures affecting plants growing season,
flowering time and timing of pollination by insects
have all been altered
• Studies already showing
– Mediterranean deciduous plants now leaf 16 days earlier
and fall 13 days later than 50 years ago
– Plants in temperate zones flowering time occurring earlier
in the season
– Growing season increased in Eurasia 18 days and 12 days
in N. America over past two decades
Phenological Changes
Penuelas J and Filella I 2001. Response to a warming world. Science 294: 793 – 795
References:
• Trease and Evans’, Pharmacognosy, 1986
• Trease and Evans’, Pharmacognosy, 1989
• Pharmacognosy and Pharmacobiotechnology, 2007
Any questions on ecosystem
reflection?
Any Questions
• Study Questions
• Define the following terms:
[Temperature, Altitude, ecology, radiation, irradiation,
cultivation, Propagation, grafting, budding, fermentation,
inoculation, seedling, dissector, fermentation,
fumigation, extraction, Infusion, Maceration, Percolation,
Digestion, Decoction, precipitation, distillation,
ecosystem, fossil, phenology, pollution, fertilization,
germination, guttation, etc]
Study Questions
• Respond to the following questions:
➢ Explain the term Commercialisation in terms of natural medicinal plants
utilization
➢ Plant growth and development and often the nature and quantity of secondary
metabolites are affected by which of the critical growth factors
➢ Give a detailed account of growth plant propagation process by vegetative
means
➢ Give a detailed account of growth plant propagation process by means of
chemical growth regulators
➢ Give a descriptive explanation of the plant harvesting process during the
production of natural sourced medicinal substances
➢ What factors are considered to harvest the required natural medicinal product in
quantitative and qualitative terms
➢ For preservation and protection of crude (from natural sources) drugs, ceratian
processes and environmental factors are considered. List and explain with
examples such processes and factor to render the desired quality of such crude
drug materials
➢ Give detailed description of various procedural methods that are practically used
to produce natural sourced product extracts
➢ What are the varying factors when plant diversity and evolution is considered
Study Questions
• Respond to the following questions:
➢ Describe the trends in plant diversity and evolution
➢ Give an illustrative description of terrestrial ecosystem (Biomes) with
relationship to different types, species and other varying characteristics of plants
➢ What are 6 major terrestrial ecosystems?
➢ Climate has always changed and plants have been able to migrate with these
changes. Why is modern climate change posing so many problems?
➢ What are some conservation efforts being investigated to prevent plant species
from extinction?
➢ What 4 major things influence geographic distribution of terrestrial biomes?
➢ Consider pro- and against artificial seed dispersal and transplantation into
climatically suitable regions
➢ What health value does Massive reforestation render to any living creature and
human beings
➢ New technologies that are used for plant transplant and plant transformation
processes are commercially available. Give a descriptive account of such
technological tacts that are used with examples plant breeding, bioengineering
(i.e. Toyota and the cherry shrub), fertilization, irrigation, etc)
➢ What social, economic and political needs are required for natural plant or
forestry preservation and conservation policies
Study Questions
Group work discussional questions:
➢ Explain the term Commercialisation in terms of natural medicinal plants utilization
➢ Plant growth and development and often the nature and quantity of secondary metabolites are affected
by which of the critical growth factors
➢ Give a detailed account of growth plant propagation process by vegetative means
➢ Give a detailed account of growth plant propagation process by means of chemical growth regulators
➢ Give a descriptive explanation of the plant harvesting process during the production of natural sourced
medicinal substances
➢ What factors are considered to harvest the required natural medicinal product in quantitative and
qualitative terms
➢ For preservation and protection of crude (from natural sources) drugs, certain processes and
environmental factors are considered. List and explain with examples such processes and factor to
render the desired quality of such crude drug materials
➢ Give detailed description of various procedural methods that are practically used to produce natural
sourced product extracts
➢ What are the varying factors when plant diversity and evolution is considered
➢ Describe the trends in plant diversity and evolution
➢ Give an illustrative description of terrestrial ecosystem (Biomes) with relationship to different types,
species and other varying characteristics of plants
➢ Consider pro- and against artificial seed dispersal and transplantation into climatically suitable regions
➢ What health value does Massive reforestation render to any living creature and human beings
➢ New technologies that are used for plant transplant and plant transformation processes are
commercially available. Give a descriptive account of such technological tactics that are used with
examples plant breeding, bioengineering (i.e. Toyota and the cherry shrub), fertilization, irrigation, etc)
➢ What social, economic and political needs are required for natural plant or forestry preservation and
conservation policies

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4 1-commercialization of natural medicinal substances

  • 2. Why Commercialisation? • Increased for domestic needs • Research and Development process • Profit creation and gains • Medical use value additions • etc
  • 4. • Plant growth and development and often the nature and quantity of secondary metabolites are affected by the following: ➢Temperature, ➢Rainfall, ➢Aspect length of day that includes quality of light , ➢Type of soil and ➢Altitude
  • 5. • Studies have shown that cannabis grown in England are reach in CBD (cannabidiol) and devoid of THC (tetrahydrocannabinol)
  • 6. TEMPERATURE • Temperature is a major factor controlling the development and metabolism of plants • Each species has become adapted to its own natural environment • Plants are able to exist in a considerable range of temperature • Many tropical and subtropical plants will grow in temperate regions during summer months, but lack frost resistance to withstand winter
  • 7. • The highest temperatures are experienced near the equator, but as the temperatures fall about 1°c for every 200m of elevation, it is possible in say Jamaica to have a tropical climate on the coast and become temperate in the mountains • The annual variations in temperature for Jamaica can be just as important as the temperature in the hottest month in the year as from the cooler part of the same year
  • 8. • Formation of volatile oils in Hyoscyamus muticus is affected by temperature variation • Logically, hot days may lead to excess physical loss of volatile oils • This would mean each plant will have the mean optimum temperature for production of volatile oil.
  • 9. RAIN FALL • The important effects of rainfall on vegetation must be considered in relation to the following: ➢ annual rainfall pattern, ➢ its distribution pattern through out the year, ➢ its effect on humidity and ➢ its effect coupled with the water holding properties of the soil
  • 10. • Variable results have been reported for the production of volatile oils under different conditions of rainfall and the development of glandular hairs( trichomes) • Continuous rainfall may lead to excess loss of water soluble substances from the leaves and roots by leaching, this is particularly common in alkaloids and glycosides even volatile oils • This could account for low production of the active constituents in wet seasons from plants
  • 11. Length of the day and Radiation Characteristics • Plants vary much in both the amount and intensity of light which they require • Research has shown that light is a factor which helps to determine the amount of glycosides or alkaloids produced • With Cinchona ledgeriana, full sunshine gives higher content of alkaloids than that produced in the shade
  • 12. • During the summer when the days tend to be longer, production for Cinchona ledgeriana could be better than the winter days • Experiment indicate that with Datura stramonium vartatula, long exposure to intense light brought a sharp increase in hyoscine content at the time of flowering • Irradiation of intact plants of Cantharatus roseus with ultraviolet light in the range of 290-380 nm (peak 370 nm) stimulates the synthesis of dimeric alkaloids probably by inducing catharathine oxidation as a trigger reaction
  • 13. • It has been shown that on long day condition peppermint leaves contain methone, menthol, and traces of menthofuran • Plants grown under short day conditions contain menthofuran as a major component of the volatile oil • Long photo period for young leaves activates the reduction pathway with conversion of menthone to menthol • Presence or absence of light together with wavelength range, have a marked effect on the secondary metabolite production of some plants in tissue culture
  • 14. Altitude • The coconut palm needs a marinetime climate and the sugar cane is a lowland plant ➢Tea - 1000-20000m, ➢cocoa - 100-200m, ➢coffee - 800-1800m ➢Tragacanth and Cinchona require elevated growing land ➢Cinchona succirubra will grow in low land but produces no alkaloids
  • 15. • The bitter constituent of Gentiana lutea increase with altitude • alkaloid of Lobelia inflata will decrease and so will the oil content of peppermint at certain altitude • Pyrethrum gives the best yields of flowering head and pyrethrins at high altitudes or near the equator
  • 16. Atmospheric composition • Digitalis lanata grown in greenhouses with carbon dioxide –enriched atmosphere (1000 p.p.m.CO2 during the whole of the growth period produce 3.5 times more the amount of digoxin, that normally would be produced
  • 17. Cultivated and wild plants • Certain plants are obtained exclusively from cultivated plants • These include Indian hemp (cannabis sativa), ginger, peppermint, spearmint, cinnamon, opium, cinchona • In some cases both wild and cultivated are used • Owing to the demand and sparse distribution or inaccessibility ,difficulty in collection, cultivation becomes essential
  • 18. • Indian hemp and opium are subjected to legal control • In many cases cultivation is advisable because of the improved quality of the drug with which it is possible to produce • The improvement may be due to the following:
  • 19. • The power to confine collections to species varieties or hybrids which have the desired character e.g. cinchona,cinammon • The better development of the plants owing to improved conditions of the soil, pruning ,and control of insect pests , fungi • The better facilities for treatment after collection e.g. drying at correct temperatures ,and peeling of ginger
  • 20. • For success in cultivation it is necessary to study the conditions under which the plant flourishes in the wild state and reproduce these conditions or improve on them • Small changes in ecology can affect plant products
  • 21. SOIL • Different plant species vary enormously in their soil and nutritive requirements • Three basic characteristics of soil are the: ➢physical, ➢chemical and ➢microbiological properties • Variation in particular size results in different soils ranging from clay, via sand, to gravel
  • 22. • Particle size is one factor influencing water holding capacity , and some plants e.g. Althaea officinalis which produce mucilage as a water retaining material contain less mucilage when grown on soil with high moisture content. • In moist regions such as Western Europe, clay soil absorb water beyond their absolute capacity
  • 23. • Their high moisture content make them cold i.e. they heat up slowly • In drier regions such as the Mediterranean's soils have the capacity to absorb and retain moisture • The basic soil type can be modified by the presence of humus, organic fertilizers, chalk lime etc.
  • 24. • Fine soil rich in humus and having a permeable substratum posses a degree of humidity which is favorable for plants • Sandy soil are suitable for xerophilous (plant adapted for life with a limited supply of water) plants • Soils containing much humus and little lime are inclined to be more acidic while those with abundant lime are alkaline
  • 25. • All plants require calcium for their normal nutrition • The effect of nitrogen-fertilizers as soil nutrients will increase the size of the plant and the amount of total alkaloids and glycosides produced
  • 27. Growth Propagation from seeds • To ensure success the seed must be collected when perfectly ripe • If not planted immediately they must be properly stored in a cool dry place and must not be kiln (artificial) dried • Some seeds like cinnamon cocoa and nutmeg lose their power to germinate if allowed to dry or if stored for quite short periods • In some, long storage decrease the percentage of germination e.g. some cereals and nuts
  • 28. • Seed can be sown at different time of the year • Some seeds are sown in spring while others can be immediate as fresh seed e.g. colchicum autumnale. If dried just for a few days only 5% will germinate in a year • Seeds slow to germinate can be soaked in water or a solution of Geberrilic acid solution for 48 hrs before sowing to increase the chances of germination
  • 29. Growth Propagation by vegetative means 1. By the development of bulbs squill, corms, e.g. colchicum, tubers e.g. jalap or rhizome e.g. ginger 2. By division, a term usually applied to the separation of a plant which has a number of aerial stems or buds into separate parts each having roots and a growing point this method may be used for Althaea, Gentiana
  • 30. 3. By runners or offsets e.g. chamomile and mints 4. By suckers or stolon e.g. liquorice and valeriana 5. By cuttings or portions of the plant severed from the plant by use of rooting hormones - Cuttings may be employed for the propagation of mints, lavender, rosemary
  • 31. 6. By layers: A layer is a branch or shoot which is induced to develop roots before it is completely severed from the parent plant - This is done by partly interrupting the food supply by means of a cut and embedding the part
  • 32. 7. By grafting and budding: Grafting is an operation which two cut surfaces usually of different but closely related plants are placed so as to unite and grow together • The rooted plant is called the stock and the portion cut off is the scion or graft e.g. Cinchona • Budding consist of the introduction of a piece of bark bearing a bud into a suitable cavity or T-shaped slit made in the bark of the stock • Budding is used in citrus spp
  • 33. 8. By fermentation : This process applies to the production of moulds and bacteria and is used for the manufacture of antibiotics and some vitamins 9.By inoculation : Specific to ergot whereby spores are of the fungus are artificially cultured and injected into the rye by special machines 10.By cell culture: The same example as for ergot spores.
  • 34. Propagation by means of Chemical Growth Regulators • The growth and development of plants may be regulated by a number of intentional added chemical substances such as: ➢Auxins ➢Gibberellins ➢Cytokinins ➢Abscisic acid ➢Ethelyne
  • 35. • These substances occur naturally in all plants • They are specific in their action and are very active in very low concentrations • They regulate cell enlargement, cell division, cell differentiation, organogenesis, senescence and dormancy • Other hormones are concerned with flowering formation and reproduction • The essential role of these hormones is illustrated in cell and tissue culturing
  • 36. Auxins • These are growth-promoting substances which are divided into the following: ➢Auxin-a ➢Auxin –b • They are obtained from human urine and cereal products • They have similar properties to indole 3- acetic acid (IAA)
  • 37. • Auxins are found in actively growing tissues and is derived in the plants from tryptophan • Effects of auxins are cell elongation giving an increase in stem length of root growth adventitious roots production and fruit setting in the absence of pollination • Synthetic auxins include: ➢Indole-3-butyric acid (I-3-BA) ➢Naphthalene-1-acetic acid (NAA) ➢2,4-dichlorophenoxyacetic acid (2,4-D)
  • 38. USES FOR AUXINS 1. In low concentrations to accelerate the rooting of woody and herbaceous cuttings 2. (Placed for 24hrs in a 1: 500,000 solution of NAA, cuttings develop roots) 3. In higher concentrations to act as selective herbicides or weed-killers
  • 39. 4. Seedlings of Mentha piperitta when treated with derivatives of NAA increased the yield 30-50% of oil which contained 4.5%-9% menthol 5. Auxins on Datura stramoniun showed an increase in production of trichomes, production of smooth fruits but there was no increase production of the tropane alkaloids
  • 40. Gibberellins • Discovered by Japanese worker in connection while working in the fields of rice. • This was during the the disease of rice fields • The effect on the plant is excessive elongation the plant to fail to support itself • The causative organism of the disease is Gibberella Fugikuroi
  • 41. • Gibberellins are distinguished as GA1,GA2,GA3……. • GA3 commonly known as gibberellic acid and is produced commercially by fungal cultivation • Gibberellins are synthesized in leaves and they accumulate in large quantities in the immature seeds and fruits
  • 42. • Application of gibberellins to short –node plants produce rosettes of leaves e.g. Digitalis lanata and Hyocymus niger • Gibberellins initiate the synthesis of hydrolytic and proteolytic enzymes upon which seeds germinate • The growth effect of gibberellins arises by cell elongation in the sub-apical meristem region where young internodes are developing • As with auxins, gibberellins occur in plants in deactivated forms
  • 43. Effects on Alkaloids • GA treatment with the seeds assist in obtaining a uniformity germination and total emergence • The plants elongation gives 2-3 folds increase in height, rapid onset of flowers and decrease in dry weight • Cantharatus recorded a lowering of alkaloid content • Datura treated with GA showed elongation of the stems and reduced alkaloid content
  • 44. EFFECTS ON VOLATILE OIL • GA treatment of volatile oil containing plants showed changes in morphological characters • Oil of peppermint gave an increased yield by 40%
  • 45. Cytokinins • Cytokinins have specific effects on cell division (cytokinesis) • Cytokinins also regulate the pattern and the frequency of organ production as well as position and shape of the plant stem • Cytokinins promote formation of adventitious buds and shoots from undifferentiated cells in tissue culture • They have an inhibitory effects on senescence
  • 46. Abscisic acid (ABA) • These are generally referred to as Growth Inhibitors • Natural growth inhibitors are present in plants and affects bud opening, seed germination and development of dormancy • One such substance is Abscisic acid (ABA) • These act as inhibitors to shoot elongation • Sprayed on plants showed reduced height
  • 47. Ethylene • Ethylene induces growth Reponses in plants • Ethylene is synthesized in plants from S- adenosylmethonium • However, it has been found to inhibit the growth of potato shoots
  • 49. COLLECTION • Drugs may be collected from the wild or cultivated plants • It is known that the active constituents of medicinal plants are affected by many factors and may vary during the course of plant growth. • This also requires skilled and unskilled labour
  • 50. Collection of medicinal plants • The season at which the drug is collected is as important as the environment of growth - Proper time of collection is very important to obtain a drug of a good quality. • The amount of the active constituents is not constant through out the year • The age of the plant is also important as it would depend on the time of collection
  • 51. • The age governs the total quantity of the active constituent produced but also the relative proportion of the components of the active mixture • Composition of the secondary metabolites varies throughout the day and night • Leaves are collected when the flowers begin to open
  • 52. • Under ground organs are collected as the aerial part die down e.g. nuts • Leaves flowers and fruits should not be collected when covered with dew or rain • Any plant attacked by mould or insects should not be collected • It is almost impossible to collect one part of the plant without collecting other parts
  • 53. • A certain percentage of stalks parts are allowed according the official monographs as “foreign matter” as defined by the BP or EP • Special machines are used to collect ergot and lavender • Reaping machines are sometimes used as compared to manual or human labour • Barks are usually collected after a period of damp weather which makes it easier for the bark to separate from the wood
  • 54. • Gums and gum resins are collected in dry weather • Care must be taken when collecting to reduce debris • Underground organs must be free from soil and must be dug in dry weather e.g. potate • Certain under ground organs must be washed and others are peeled e.g. carotine
  • 55. Factors affecting collection 1. Time of the year: The plant may contain a substance in winter that is not present in summer, or its amount varies markedly e.g. Rhubarb contains no anthraquinone in winter, instead it contains anthranols, which in summer, are oxidized to anthraquinones. Colchicum corm is free from bitterness and is devoid of the alkaloid colchicine in autumn, hence is used in Austria as a food, instead of potatoes. Bitterness starts to appear in spring and early summer when it is used as a drug.
  • 56. 2- Time of the day: Some drugs, like Digitalis, contain different amounts of active constituents at different times of the day. Being highest in the afternoon.
  • 57. 3- Stage of maturity and age: • The value and content of active constituents of many drugs depends on the stage of maturity and age. • Conium fruits contain coniin when fruits are mature and unripe. • Santonica flowers are rich in santonin, when unexpanded, when it starts to open, the santonin content decreases.
  • 58. Drying of crude drugs Reasons for drying: 1. To help in their preservation. 2. To fix their constituents, by preventing reactions that may occur in presence of water. 3. To prevent the growth of micro-organisms such as bacteria and fungi. 4. To facilitate their grinding. 5. To reduce their size and weight. 6. Insufficient drying favors spoilage by micro- organisms and makes it possible for enzymatic destruction.
  • 59. • If enzymes’ action is to be encouraged slow drying at moderate temperatures is necessary e.g. cocoa • If enzymatic action is not desired drying should take place as soon as possible after collection • Drugs containing volatile oil are liable to lose their aroma if not dried or if the oil is not distilled from them immediately • All moist drugs are likely to develop mould if left long in a dumpy environment
  • 60. • For this reason drying apparatus and stills should be situated as near to the growing plants as possible • The duration of drying varies from a few hours to weeks • Open air drying is used for cloves, cinnamon • For drying in shades drugs may be suspended in bundles
  • 61. • Trays are also used that are made of wire mesh • Drying by artificial heat is used where rapid drying is desired or where humidity is high • Continuous belts are used for drying large crops such as digitalis • Heat may also be applied as open fires, by use of stoves and hot water pipes • In all drying sheds the space between the trays should be 15cm apart
  • 62. • Rapid drying of flowers helps them retain their colour and aromatic drugs also retain there aroma • Leaves, herbs and flowers may be dried at 20-40c • Barks and roots slightly higher than that of leaves as above, at 30-65c
  • 63. Methods of drying Drying is carried out either by natural or artificial methods. 1- Natural drying: this is accomplished by natural air in sun or shade. 2- Artificial drying: this is a rapid method done at well-controlled temperature and is accomplished by: • direct fire. • Use of heated stones. • Use of stoves.
  • 64.
  • 65. • Lyophilization (Freeze drying): Frozen material is placed in an evacuated apparatus which has a cold surface maintained at -60 to -80 °C. Water vapour from the frozen material passes rapidly to the cold surface. It is used for drying heat-sensitive substances e.g. antibiotics and proteins. • Chemical drying using desiccators • An absolutely dried drug is that completely freed from water, when exposed to air it absorbs 8-10% of moisture and is called air-dry drug.
  • 66.
  • 67. Stabilization • On long storage, enzymatic reactions will slowly destroy the constituents, because the last traces of water can never be removed. • In order to avoid this degradation, the enzymes should be destroyed before drying, a process usually called stabilization. • The most common method being brief exposure (a few minutes only) of the plant material to ethanol vapor under pressure (0.5 atm).
  • 68. Fermentation • Enzymatic transformation of the original plant constituents is sometimes desirable. e.g. Vanilla pods • The fresh material is placed in thick layers, sometimes covered and often exposed to raised temperatures (30-40 °C) and humidity, so as to accelerate the enzymatic processes. • The fermented product must be dried afterwards to prevent attack by microorganisms, e.g. moulds.
  • 69. Preservation and protection of crude drugs Storage represents the last stage of preparing crude drugs. Drugs usually deteriorate along the time of storage, except in few cases e.g. Cascara and Frangula should not be used except after certain period of storage. Certain drugs such as Nux vomica are hardly affected by storage. Generally, changes that take place during storage of crude drugs are objectionable, e.g. drugs containing volatile oils gradually lose their aroma. Improper methods of storing and inadequate protection during storage can cause a pronounced deterioration. There are two principal reasons for deterioration: Physiochemical: moisture, heat, air and light. Biological: fungi, bacteria, insects and rodents.
  • 70. Physicochemical factors 1. Moisture: moisture sometimes affects drugs adversely through activating the enzymes (as in cardiac glycosides). 2. Heat: rise of temperature up to 45 activates the enzymes causing decomposition of active constituents. Volatile oil containing drugs are also affected by higher temperatures, their content decreases. 3. Air: oxygen of air oxidizes certain constituents of crude drugs, e.g. linseed and lemon oil, it causes rancidity of fixed oils and resinification of volatile oils. 4. Light: it affects drugs, especially those having marked colours.e.g. yellow colour of Rhubarb changes to reddish tint, white coloured corollas turn brown.
  • 71. Biological factors 1. Bacteria: cotton fibres are rendered brittle by bacterial attack which makes the cotton wool objectionable and dusty. 2. Moulds: the mycelium of delicate hyphae produces an unpleasant mass of clinging particles in powdered drugs. 3. Insects: they seem to attack all drugs but have preferences to certain drugs as ginger, belladonna, kola, liquorice - Insects which infest vegetable drugs include beetles, mites and moths. They render drugs porous and powdery. 4. Rodents: they cause much spoilage of crude drugs during storage, especially if wrapped in paper, cloth or put in cardboard or wooden containers. The presence of rodent's filth, excreta, hairs causes rejection of the drug.
  • 72. Methods for controlling insects 1. Heat treatment: it is the simplest method and is done by exposing the drug to a temperature of 60-65. it is effective especially for insect eggs which are not affected by insecticides. 2. Fumigation: this is done by volatile insecticidal agents in closed areas e.g. CCL4, CS2, CN. Most fumigants do not kill eggs of insects. It is advisable to repeat fumigation at intervals to obtain better results.
  • 73. 3. Liming: liming of certain drugs as ginger, nutmeg to protect against insect attack provides only partial protection. 4. Low temperature storage: this method is preferred to fumigants and liming. Adult insects, pupae, larvae and eggs are sometimes killed by very low temperatures. 5. Exposure to alternate periods of low and high temperatures: frequently is more effective for killing insects than a prolonged period of low temperature exposure.
  • 75. Grinding of crude drugs • Regardless of whether the crude drug is to be used for isolation of a pure compound or for manufacture of a simple preparation, the first operation that must be performed is grinding of the plant material to a powder of suitable particle size. • It is important that the particles are of as uniform size as possible. • Excessive dust can clog percolators and result in a turbid extract which is hard to clarify.
  • 76. • Large particles take a longer time for complete extraction than small ones. • Large differences in particle size thus slow down the extraction process. Types of machines used for grinding crude drugs: 1. Hammer mill; a common type for grinding crude drugs. 2. Knife mill; is useful for production of low-dust powders of leaves, barks and roots for subsequent percolation or maceration. 3. Tooth mill; is used for production of very fine powders.
  • 77. Notes: • Grinding produces a certain amount of heat which must be observed when grinding crude drugs containing heat-sensitive compounds. • Mills cooled with liquid nitrogen are available for such purposes. • Cold grinding is also preferable for crude drugs containing volatile oils. • Following grinding, the material must be sifted to ensure the proper particle size.
  • 78. Extracts • Extracts can be defined as preparations of crude drugs which contain all the constituents which are soluble in the solvent used in making the extract. • In dry extracts all solvent has been removed. • Soft extracts and fluid extracts are prepared with mixtures of water and ethanol as solvent. • Tinctures are prepared by extraction of the crude drug with five to ten parts of ethanol of varying concentration, without concentration of the final product.
  • 79. • Plant constituents are usually contained inside the cells. Therefore, The solvent used for extraction must diffuse into the cell to dissolve the desired compounds whereupon the solution must pass the cell wall in the opposite direction and mix with the surrounding liquid. • An equilibrium is established between the solute inside the cells and the solvent surrounding the fragmented plant tissues.
  • 80. The speed with which this equilibrium is established depends on: 1. Temperature 2. pH 3. Particle size 4. The movement of the solvent
  • 81. Choice of solvent • The ideal solvent for a certain pharmacologically active constituent should: 1. Be highly selective for the compound to be extracted. 2. Have a high capacity for extraction in terms of coefficient of saturation of the compound in the medium. 3. Not react with the extracted compound or with other compounds in the plant material. 4. Have a low price. 5. Be harmless to man and to the environment. 6. Be completely volatile.
  • 82. • Aliphatic alcohols with up to three carbon atoms, or mixtures of the alcohols with water, are the solvents with the greatest extractive power for almost all natural substances of low molecular weight like alkaloids, saponins and flavonoids. • According to the pharmacopoeias, ethyl alcohol is the solvent of choice for obtaining classic extracts such as tinctures and fluid, soft and dry extracts.
  • 83. • The ethanol is usually mixed with water: 1- to induce swelling of the plant particles 2- to increase the porosity of the cell walls which facilitates the diffusion of extracted substances from inside the cells to the surrounding solvent. • For extraction of barks, roots, woody parts and seeds the ideal alcohol/water ratio is about 7:3 or 8:2. • For leaves or aerial green parts the ratio 1:1 is usually preferred in order to avoid extraction of chlorophyll.
  • 84. Extraction Procedures There are many procedures for obtaining extracts 1- Infusion 2- Maceration 3 Percolation 4- Digestion 5- Decoction 6- Continuous hot extraction 7- Solvent-solvent precipitation 8- Liquid-liquid extraction 9- Distillation 10- Specific procedures
  • 85. STORAGE • Long storage for herbal drugs is not recommended • Indian hemp deteriorates with long storage • Drugs can be stored in sacks, cardboard boxes ,wooden cases and paper bags which, enables them to reabsorb about 10- 20% surrounding moisture • These are termed air dry materials with such moisture percentage content • Plastic sacks will seal effectively the contents
  • 86. • Permissible moisture for starch ,acacia gum is found in standard International Pharmacopeias such as EP, BP, USP etc • A combination of moisture and temperature on humidity and the subsequent water condensation when temperature falls must be considered in during storage as it can be detrimental to the substances • They must be kept in sealed containers with dehydrating agent
  • 87. • For large quantities the bottom of the box may be filled with quicklime and separated from the drug by perforated grid or sackings • Volatile oil should be sealed in well-filled containers and in cool dark places
  • 90. Terrestrial Ecosystem Response to Climate Change – This is just a reminder of the global natural system changes on global natural medicinal plants distribution Terrestrial Ecosystem (Biomes)
  • 91. Global Change and Effects on Terrestrial Ecosystem Introduction • Temperature, precipitation, latitude and altitude all determine distribution of major terrestrial ecosystems (biomes). • Plants found within the different biomes are influenced by soil type, water shed conditions and amount of sun. • Specific combinations of temperatures and precipitation ensure the survival and thriving of plants within a given environment (known as Climate Space and Change).
  • 92. Terrestrial Ecosystems are an… • Integral part of global carbon system • As earlier stated that plants take in and store carbon dioxide from the atmosphere through photosynthesis • Below ground microbes decompose organic matter and release organic carbon back into the atmosphere Cycle shows how nature’s sources of CO2 are self regulating – that which is released will be used again – Anthropogenic carbon not part of natures cycle – is in excess www.bom.gov.au/.../ change/gallery/9.shtml
  • 93. Forests • Forests occupy major portions of land mass in different countries. – In the U.S. forests occupy 33% of the land mass while in Africa, natural forestry occupy over 60% of the land • Forests have many functions: – Provides habitat for plants and animals – Influence amount of and availability of water runoff – Provide sites for recreation – Provide timber for harvesting lumber; wood pulp, fire wood for fuel – Total commercial estimated valued in U.S. = $290 billion and more than twice for Africa
  • 94. Land Formation • 18,000 years ago glaciers retreated in the Northern hemisphere resulting in a rocky and lifeless ecosystem • Pioneer plants scientifically may have emerged due to the following: – Lichens – broke down rock – Decaying lichens mixed with broken rock chips developed soil (long/slow process) • Winds blew – soil flew – dispersed into crevices of rocks – provided nutrients for small plants to grow • Soon mosses appeared followed by grasses, then larger plants (shrubs & trees) known as primary succession
  • 95. Succession and Climax Forests •Primary succession - How temperate forests first emerged •As forests continue to grow – become more diverse in both plant and animal life •Simple plants unable to compete – live in the shade of larger plants •Saplings unable to develop due to shade - slowly die out (Birch, Aspen) – gives rise to middle stage succession •Shade tolerant plants emerge (Maple, Hemlock) and dominate forests – now have a climax forest
  • 96. Secondary Succession • Secondary succession occurs quicker than primary succession (soils already there) – Exception to this – land cleared for development and agriculture • Temperate forests dominate because of their resistance to fires • Less resistant trees eventually die off – leaving the evergreens still standing • Fires promote evergreen growth by busting seed coats open and scattering them in the soil • Deciduous forest devastated by fires • Secondary succession only way new forest will dominate people.eku.edu/ritchisong/ secondarysuccession
  • 97. A look back into time….. Driving forces effecting global temperature; •Plate tectonics •Earths orbital geometry (eccentricity, obliquity and precession)
  • 98. Billions of years ago Major continental shifts – earth extremely hot Underwent cycles of glaciations ice sheets existed at lower latitudes Paleozoic Era Interglacial period plants invade land Permian Major Glaciation Paleocene Elevated greenhouse gases warmed up planet Palm trees in Alaska Crocodiles in the Arctic 610 – 575 Ma 438 – 408 Ma 292 - 250 Ma 55 – 52 Ma Antarctic ice sheets formed 34 Ma Pleistocene Ice Age 1 Ma 1.8 Ma – 18 Kya Holocene – cycle of glaciation and melting of ice caps – rising sea levels 18 Kya Last glacial period
  • 99. Changes in Global Climate 65 ma to Present Asteroid hit Chesapeake led to major glaciation period
  • 100. Billions of years ago Major continental shifts – earth extremely hot Underwent cycles of glaciations ice sheets existed at lower latitudes Paleozoic Era Interglacial period plants invade land Permian Major Glaciation Paleocene Elevated greenhouse gases warmed up planet Palm trees in Alaska Crocodiles in the Arctic 610 – 575 Ma 438 – 408 Ma 292 - 250 Ma 55 – 52 Ma Antarctic ice sheets formed 34 Ma Pleistocene Ice Age 1 Ma 1.8 Ma – 10 Kya Holocene – cycle of glaciation and melting of ice caps – rising sea levels 18 Kya Last glacial period
  • 101. Time Line of Plant Life Paleozoic era (438 – 408 Ma) Bryophytes Cretaceous era (110 Ma) Angiosperms dominate Carboniferous (360 – 286 Ma) Ferns, Gymnosperms Liverworts Hornworts Mosses taggart.glg.msu.edu/isb200/fland.htm
  • 102. Jurassic (213 – 144Ma) → Cretaceous (144 – 65 Ma)
  • 103. 570 505 438 408 360 286 245 208 144 66 2 10K Million years before present Global Temperature
  • 104. Paleogeographic Biome Late Jurassic (150 Ma) www.geo.arizona.edu/ ~rees/Jurassic.html Five Main Biomes: • winter wet (seasonally dry) • summer wet (subtropical) • desert • warm temperature • cool temperate ▪ Fossils of plant life used to reconstruct climate biomes summerwet (subtropical) warm temperature cool temperature winter wet (seasonally dry) desert
  • 105. 570 505 438 408 360 286 245 208 144 66 2 10K Million years before present
  • 106. Prentice, C.I., Guiot, J., Huntley, B., Jolly D. and Cheddadi, R., 1996, Reconstructing biomes from palaeoecological data: a general method and its application to European pollen data at 0 and 6 ka. Climate Dynamics 12:185-194. Global Distribution of Vegetation 18,000 years ago conifers tundra taiga grassland woodland desert
  • 107. Prentice, C.I., Guiot, J., Huntley, B., Jolly D. and Cheddadi, R., 1996, Reconstructing biomes from palaeoecological data: a general method and its application to European pollen data at 0 and 6 ka. Climate Dynamics 12:185-194. Global Distribution of Vegetation 6,000 years ago taiga temperate deciduous woods & scrub conifers grassland desert tundra cold deciduous
  • 108. Prentice, C.I., Guiot, J., Huntley, B., Jolly D. and Cheddadi, R., 1996, Reconstructing biomes from palaeoecological data: a general method and its application to European pollen data at 0 and 6 ka. Climate Dynamics 12:185-194. Global Distribution of Vegetation - Present taiga tundra temperate deciduous grassland cold deciduous tropical R.F. warm mix
  • 109. 18 kya 9 kya Present Distribution of spruce and oak forests in Northern Hemisphere since the glacial period 18,000 kya spruce spruce spruce spruce spruce ice sheet ice ice ice ice oak oak oak oak • 18,000 years ago Spruce trees and oak trees filled small pockets of habitat – as climates warmed Spruce trees migrated into the Northern Hemispheres and the Oak trees expanded in to Southeastern U.S., Western Europe and Southern Europe • Shifts in vegetation occur slowly tree species were able to successfully expand into favorable regions Shifts in Terrestrial Habitat 18Kya 9Kya Present
  • 110. Major Biomes and Their Vegetation • Tundra – no trees, lichens, grasses and shrubs • Taiga (or Boreal Forest) – coniferous evergreens • Temperate forests – include evergreens (spruce), deciduous forests (oaks), mixed forests, and temperate rain forests (sequoias) • Tropical rain forests – greatest amount of diversity in vegetation (vines, orchids, palms) • Grasslands – grasses, prairie clover • Deserts – cacti, small bushes
  • 111. Major Terrestrial Biomes • Geographic distribution of biomes are dependent on temperature, precipitation, altitude and latitude • Weather patterns dictate the type of plants that will dominate an ecosystem faculty.southwest.tn.edu/. ../ES%20%20we16.jpg
  • 112. Major Terrestrial Biomes • Geographic distribution of biomes are dependent on temperature, precipitation, altitude and latitude • Weather patterns dictate the type of plants that will dominate an ecosystem faculty.southwest.tn.edu/. ../ES%20%20we16.jpg
  • 114. Alaska Greenland Ice land Siberia Canada Location: Regions south of the ice caps of the Arctic and extending across North America, Europe and Siberia (high mountain tops) Yakutsk Tundra N. Europe Average Temperature: -40°C to 18°C Average Precipitation: 150 to 250 mm of rain per year Type of vegetation: Almost no trees due to short growing season and permafrost; lichens, mosses, grasses, and shrubs
  • 115. Yakutsk, Russia Location: 62.1 N; 129 W earthobservatory.nasa.gov Average annual temperature and precipitation
  • 116. Temperature: -40°C to 20°C, average summer temperature is 10°C Precipitation: 300 to 900 millimeters of rain per year Vegetation: Coniferous-evergreen trees Location: Canada, Europe, Asia, and the United States Other: Coniferous forest regions have cold, long, snowy winters, and warm, humid summers; well-defined seasons, at least four to six frost- free months Boreal Forest (Taiga) Canada Europe Asia S.W. U.S
  • 117. Beaverlodge, Alberta, Canada earthobservatory.nasa.gov Boreal Forest (Taiga) Average annual temperature and precipitation 55oN; 119o W
  • 118. Average Yearly Temperature: Between -30°C to 30°C; hot summers, cold winters; sunlight varies between seasons Average Yearly Precipitation: 750 to 1,500 mm of rain per year Vegetation: Deciduous - Broadleaf trees (oaks, maples, beeches), shrubs, perennial herbs, and mosses. Evergreen – (N. America) – Spruce, Hemlock, Pine and Fir trees Temperate Rainforests – (CA, OR, & WA) – made up of Red wood and Sequoias www.windows.ucar.edu/.../ earth/forest_eco.html Four types: 1. deciduous forests 2. evergreen forests 3. mixed deciduous and evergreen 4. temperate rain forests Location: Eastern United States, Canada, Europe, China, and Japan Temperate Forests
  • 119. Staunton, Virginia, United States 38oN; 79oW earthobservatory.nasa.gov Average annual temperature and rainfall
  • 120. Tropical Forests General Characteristics: Average Temperature: 20°C to 25°C, must remain warm and frost-free Average Precipitation: 2,000 to 10,000 millimeters of rain per year Average Humidity: Between 77 – 88% Types of Vegetation: Large trees reaching up to 240 feet, have the most diverse trees than any other biome: vines, orchids, ferns Tropical rainforests: • Cover less than 6% of Earth’s land surface • Produce more than 40% of Earth’s oxygen • Contain more than half of all the worlds plants and animals • ¼ of all medicines come from rainforest plants • Scientists believe more than 1400 tropical plants thought to be potential cures to cancer
  • 121. 3 major geographical areas: 1. America: Amazon river basin 2. Africa: Zaire basin, small area of W. Africa, Eastern portion of Madagascar 3. Asia: West coast of India, Assam, S.E. Asia, New Guinea and Queensland, Australia Tropical Rainforest Amazon river basin Zaire Madagascar India S.E. Asia Queensland New Guinea "Rainforests", http://passporttoknowledge.com/rainforest/GEOsystem/Maps/se_asia.html, (3/18/02)
  • 122. Campa Pita, Belize 15 N latitude earthobservatory.nasa.gov Tropical Forest Average annual temperature and precipitation
  • 123. Southeast Asia Tropical Rainforest Monsoons role • SE Asia has a tropical wet climate which is influenced by ocean wind systems originating in the Indian Ocean and China Sea • 2 monsoon seasons: – Northeast monsoons (Oct. – Feb) – bring heavy rains to Eastern side of the islands – Southwest monsoons (April – Aug) – more powerful of the two seasons – brings heavy rainfall to the western side of the islands – Eastern side of islands dry – but windy (due to rain shadow) • Change in monsoon cycle bring heavy consequences – Ex. 1992 – 1993 – logging degraded primary foresting making it vulnerable to fires. A drought brought on by El Nino created devastating fires destroying 27,000 km2 of acreage. – In 1998 the same type of thing happened again when El Nino created a weak monsoon season – destroying many plant and animal species.
  • 125. Location: The prairies of the Great Plains of North America, the pampas of South America, the veldt of South Africa, the steppes of Central Eurasia, and surrounding the deserts in Australia Temperature: Dependent on latitude, yearly range can be between -20°C to 30°C Precipitation: About 500 to 900 mm of rain per year Vegetation: Grasses (prairie clover, salvia, oats, wheat, barley, coneflowers) Other: Found on every continent except Antarctica Grasslands
  • 126. Ingeniera White, Argentina 40oS; 6oW earthobservatory.nasa.gov Average annual temperature and rainfall
  • 127. Location: Found north and south of the Equator Temperature: Average of 38°C (day), average of -3.9°C (night) Precipitation: About 250 mm of rain per yr Vegetation: Cacti, small bushes, short grasses Other: Perennials survive for several years by becoming dormant and flourishing when water is available. Annuals are referred to as ephemerals because some can complete an entire life cycle in weeks. Deserts
  • 128. El-Oasr el-Akhdar, Egypt 26oS; 30oE earthobservatory.nasa.gov Average yearly temperature and rainfall
  • 129. So … what are the predictions????? Present day Predicted Distribution for est for est grassland grassland ari d woodl and shrub land shrub land woodl and grassland grassland Climate change p. 104 • Arid deserts in Southwestern U.S. will shrink as precipitation increases • Savanna/shrub/woodland systems will replace grasslands in the Great Plains • Eastern U.S. – forests will expand northerly – weather conditions will become more severe • Southeastern U.S. – increasing droughts will bring more fires – triggering a rapid change from broadleaf forests to Savannas
  • 130. Predicted Change in Biomes Loss of existing habitat that could occur under doubling of CO2 concentration. Shades of red indicate percentage of vegetation models that predicted a change in biome type.
  • 131. Distribution of Sugar Maple in Eastern North America will change due to an increase in temperature and a decrease in moisture shifting further north east. present range Prediction based on Prediction based on increased increased temperature temperature and decrease precipitation present range Predictions of Sugar Maple in Eastern North America overlap predicted new growth predicted new growth overlap
  • 132. Wet western slope will shrink and be replaced by pine and oak Eastern slope will become drier and shift to Juniper and Sagebrush More Predictions Western Hemlock and Douglas fir found on Western slope ❑ Douglas Fir found in wet coastal mountains of CA and OR will shrink in low lands and be replaced by Western pine species which are more drought tolerant. ❑ Overall Western U.S. climate is predicted to shift to favor more drought tolerant species of pine
  • 133. Frequency of forest fires will increase, reducing total American boreal forest area.
  • 134. Shifts in Terrestrial Habitat • It is predicted that at the end of this century there will be large scale shifts in the global distribution of vegetation in response to anthropogenic climate change. • With man doubling the amount of carbon dioxide entering into the atmosphere the climate is changing more rapidly then plant migration can keep up. Potential distribution of the major world biomes under current climate conditions Projected distribution of the major world biomes by simulating the effects of 2xCO2-equivalent concentrations www.usgcrp.gov/usgcrp/ seminars/960610SM.html
  • 135. Boreal and Alpine Vegetation • Research indicates the greatest amount of change will occur at the higher latitudes • Northern Canada and Alaska are already experiencing rapid warming and reduction of ice cover • Vegetation existing in these areas will be replaced with temperate forest species • Tundra, Taiga and Temperate forests will migrate pole ward • Some plants will face extinction because habitat will become too small (ex. Mountain tops of European Alps) Predicted changes in Siberian vegetation in response to doubling of CO2 Climate change
  • 136. Grasslands and Shrub Lands • Grassland will change to deserts or shrub lands – Exposing greater amounts of soil – Increasing soil temperature – poor nitrogen content – poor plant growth – Barren soil exposed to winds and transported into atmosphere as dust and trapping IR – leading to more warming • Models of: • Climate change • Plant growth • Soil – water Predict shifts in distribution of major North American prairie grasses over a 40 year period
  • 137. Those at Risk • Northern countries (Russia, Sweden, Finland) ½ of existing terrestrial habitats at risk • In Mexico, it’s predicted that 2.4% of species will lose 90% of their range and threatened with extinction by the year 2055 • Population at greatest risk are the rare and isolated species with fragmented habitats or those surrounded by water, agriculture or human development • Polar bears facing extinction by prolonged ice melts in feeding areas along with decline in seal population
  • 138. www.sciencedaily.com/.../ 09/050918132252.htm ❑ 35% of worlds existing terrestrial habitat predicted to be altered ❑ Studies found that deforestation in different areas of the globe affects rainfall patterns over a considerable region ▪ Deforestation in the Amazon region of South America (Amazonian) influences rainfall from Mexico to Texas and in the Gulf of Mexico ▪ Deforesting lands in Central Africa affects precipitation in the upper and lower U.S. Midwest
  • 139. Phenological Changes • Life-cycles of plants and animals have been affected by global change • Temperatures affecting plants growing season, flowering time and timing of pollination by insects have all been altered • Studies already showing – Mediterranean deciduous plants now leaf 16 days earlier and fall 13 days later than 50 years ago – Plants in temperate zones flowering time occurring earlier in the season – Growing season increased in Eurasia 18 days and 12 days in N. America over past two decades
  • 140. Phenological Changes Penuelas J and Filella I 2001. Response to a warming world. Science 294: 793 – 795
  • 141. References: • Trease and Evans’, Pharmacognosy, 1986 • Trease and Evans’, Pharmacognosy, 1989 • Pharmacognosy and Pharmacobiotechnology, 2007
  • 142. Any questions on ecosystem reflection?
  • 144. • Study Questions • Define the following terms: [Temperature, Altitude, ecology, radiation, irradiation, cultivation, Propagation, grafting, budding, fermentation, inoculation, seedling, dissector, fermentation, fumigation, extraction, Infusion, Maceration, Percolation, Digestion, Decoction, precipitation, distillation, ecosystem, fossil, phenology, pollution, fertilization, germination, guttation, etc]
  • 145. Study Questions • Respond to the following questions: ➢ Explain the term Commercialisation in terms of natural medicinal plants utilization ➢ Plant growth and development and often the nature and quantity of secondary metabolites are affected by which of the critical growth factors ➢ Give a detailed account of growth plant propagation process by vegetative means ➢ Give a detailed account of growth plant propagation process by means of chemical growth regulators ➢ Give a descriptive explanation of the plant harvesting process during the production of natural sourced medicinal substances ➢ What factors are considered to harvest the required natural medicinal product in quantitative and qualitative terms ➢ For preservation and protection of crude (from natural sources) drugs, ceratian processes and environmental factors are considered. List and explain with examples such processes and factor to render the desired quality of such crude drug materials ➢ Give detailed description of various procedural methods that are practically used to produce natural sourced product extracts ➢ What are the varying factors when plant diversity and evolution is considered
  • 146. Study Questions • Respond to the following questions: ➢ Describe the trends in plant diversity and evolution ➢ Give an illustrative description of terrestrial ecosystem (Biomes) with relationship to different types, species and other varying characteristics of plants ➢ What are 6 major terrestrial ecosystems? ➢ Climate has always changed and plants have been able to migrate with these changes. Why is modern climate change posing so many problems? ➢ What are some conservation efforts being investigated to prevent plant species from extinction? ➢ What 4 major things influence geographic distribution of terrestrial biomes? ➢ Consider pro- and against artificial seed dispersal and transplantation into climatically suitable regions ➢ What health value does Massive reforestation render to any living creature and human beings ➢ New technologies that are used for plant transplant and plant transformation processes are commercially available. Give a descriptive account of such technological tacts that are used with examples plant breeding, bioengineering (i.e. Toyota and the cherry shrub), fertilization, irrigation, etc) ➢ What social, economic and political needs are required for natural plant or forestry preservation and conservation policies
  • 147. Study Questions Group work discussional questions: ➢ Explain the term Commercialisation in terms of natural medicinal plants utilization ➢ Plant growth and development and often the nature and quantity of secondary metabolites are affected by which of the critical growth factors ➢ Give a detailed account of growth plant propagation process by vegetative means ➢ Give a detailed account of growth plant propagation process by means of chemical growth regulators ➢ Give a descriptive explanation of the plant harvesting process during the production of natural sourced medicinal substances ➢ What factors are considered to harvest the required natural medicinal product in quantitative and qualitative terms ➢ For preservation and protection of crude (from natural sources) drugs, certain processes and environmental factors are considered. List and explain with examples such processes and factor to render the desired quality of such crude drug materials ➢ Give detailed description of various procedural methods that are practically used to produce natural sourced product extracts ➢ What are the varying factors when plant diversity and evolution is considered ➢ Describe the trends in plant diversity and evolution ➢ Give an illustrative description of terrestrial ecosystem (Biomes) with relationship to different types, species and other varying characteristics of plants ➢ Consider pro- and against artificial seed dispersal and transplantation into climatically suitable regions ➢ What health value does Massive reforestation render to any living creature and human beings ➢ New technologies that are used for plant transplant and plant transformation processes are commercially available. Give a descriptive account of such technological tactics that are used with examples plant breeding, bioengineering (i.e. Toyota and the cherry shrub), fertilization, irrigation, etc) ➢ What social, economic and political needs are required for natural plant or forestry preservation and conservation policies