Protein eng &amp; PCR

Protein Engineering Types PCR Principles of PCR Procedure Of PCR

Santé & Médecine

Subject: P’ceutical Biotechnology
(BP-607)
UNIT-I
Topic: Protein Engineering
&
PCR
By: Ms.Uditi Handa (Asst. Prof.)
GGSCOP, Yamunanagar

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Tablet coating Sugar coating & its Steps involved for preparation Film Coating Enteric Coating Equipment's used in tablet coating Types of coating Raw materials used in coating Film coating machine perforated coating pan Fluidized bed coater Problems of Film coating Solution to resolve the film defects tablet evaluation test official tests physical appearances content uniformity disintegration dissolution Unofficial test hardness test friability test different types of hardness tester

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capsules dosage form Types of Capsules hard gelatin capsules soft gelatin capsules properties of capsules advantages and disadvantages types of gelatin manufacturing of gelatin capsules filling machines filling machines principles numbers of capsules and their size filling of hard gelatin capsules different shapes of soft gelatin capsules manufacturing of soft gelatin capsules evaluation test of capsules dosage form

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Methodology of Preformulation Studies related to Pharmaceutical Sciences. Physio-chemical properties, Program of analytical preformulation. Preformulation Drug Characterization in a Structured Program. Benefits of Preformulation Studies. Bio-pharmaceutical Properties. Bulk Characterization, Solubility analysis, organoleptic properties. Stability studies Pharmaceutical studies objectives of preformulation Need of preformulation studies in Drug Characterization Solubility analysis Dissolution Electrostatic Charges method for Characterization such as UV Spectroscopy, HPLC, TLC, pH determination. Microscopy, Flow Properties Assay development, Lipophilicity pH effect Parentral Preparation Pharmacokinetic Ophthalmic Preparation Isotonicity Suspensions and emulsions Aspirin Pilocarpine penicillin Crystalline compound Amorphous Compound Bioavailability method to determine Partition Coefficient Log P Values Particle Size , Shape and Surface area. Color, taste , odour and physical appearances types of preformulation studies major area of preformulation research absorption metabolism duration of action shake flask method gas chromatography Preformulation Studies: Introduction to preformulation, goals and objectives, study of physicochemical characteristics of drug substances. a. Physical properties: Physical form (crystal & amorphous), particle size, shape, flow properties, solubility profile (pKa, pH, partition coefficient), polymorphism b. Chemical Properties: Hydrolysis, oxidation, reduction, racemisation, polymerization Application of preformulation considerations in the development of solid, liquid oral and parenteral dosage forms and its impact on stability of dosage forms.

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