The rhinovirus (from the Greek ῥίς rhis "nose", gen ῥινός rhinos "of the nose", and the Latin vīrus) is the most common viral infectious agent in humans and is the predominant cause of the common cold. Rhinovirus infection proliferates in temperatures of 33–35 °C (91–95 °F), the temperatures found in the nose. Rhinoviruses belong to the genus Enterovirus in the family Picornaviridae. The three species of rhinovirus (A, B, and C) include around 160 recognized types of human rhinovirus that differ according to their surface proteins (serotypes).[1] They are lytic in nature and are among the smallest viruses, with diameters of about 30 nanometers. By comparison, other viruses, such as smallpox and vaccinia, are around ten times larger at about 300 nanometers; while flu viruses are around 80–120 nm.

1. Module- 4
Topic name
RHINO VIRUS NOTES
Sub code: BMLT5001
Sub Name: Virology and mycology
Department: Department of MLT, SMAS
Faculty: A. Vamsi Kumar
Designation : Assistant professor
Sem- V

2. SYLLABUS OVERVIEW
Unit-1 Mycology 8 hours
Introduction to medical mycology, basic concepts about superficial and deep mycoses taxonomy , classification &
general characteristics of Various medically important fungi.
Unit-2 Fungal infection 8 hours
Names of fungi & diseases caused by them; superficial mycoses, candida, dermatophytes, opportunistic fungi,
subcutaneous mycoses
Unit-3 Virology 7 hours
Introduction, classification of virus, collection, Transport, & Storage of sample for Viral diagnosis. Staining Techniques
used in virology,
Processing of sample for viral diagnosis (Egg Inoculation & Tissue culture)
Unit-4 RNA Viruses 9 hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies, Arbo viruses, Measles Mumps, Rubella, HIV
Unit-5 DNA Viruses 8 hours
DNA viruses: Small Pox, HSV, CMV, EBV, Varicella Zoster, Hepatitis, Adeno virus
Unit - 6 Next generation PCR and it’s modren applications 8 Hours
PCR and Next genration DNA Secquencing techniques to identify viral genome: History of PCR, Priciple of PCR, Stages
of PCR, procedure of PCR, Concept of optimization of PCR, Applications of PCR, Advancements in PCR technology, DNA
sequecing techniques, Methods of DNA Sequencing, Next generation DNA sequencing techniques

3. Unit-4 RNA Viruses 9 hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies, Arbo
viruses, Measles Mumps, Rubella, HIV
Unit 4

4. NOTES

5. Classification / Families of viruses
DNA Viruses RNA Viruses
HAP3
Herpes viridae
1. Alpha – HS1, HS2, EBV
2. Beta – CMV
3. Gama -VZ
DNA “HAS” the potential
ii. Adenovirus
iii. Small pox
iv. Hepatitis
PR2OCBT
H H
PCR2MMR3 – IH
1. Polio virus
2. Corona virus
3. Rhino virus
4. Rota virus
5. Measles virus
6. Mumps virus
7. Rubella
8. Rabis
9. Influenza
10. HIV
AIDS

6. General points
1. Introduction
2. Classification
3. Morphology (General properties)
• Size
• Shape
• Symmetry
• Capsomers
• Genome
• Envelop
• Special viral proteins
4. Susceptibility & Resistance
5. Isolation / Cultivation - Animal/tissue/cell
culture
6. Pathogenesis + mode of transmission +
incubation period
7. Signs & Symptoms + Complications
8. Laboratory diagnosis
• Sample collection (antemortem + Post
mortem )
• Transport
• Direct microscopy
• Culture / isolation of virus
• Cytopathic effects
• Inclusion bodies or other tests
• Immunofluorescence tests
• Molecular tests
• Ag detection tests
• Detection of viral specific antibodies
9. Prophylaxis (Vaccination)
• Active immunization
• Passive immunization
10. Treatment
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

7. 1. Introduction
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

8. Other viruses that cause common cold
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

9. 2. Classification1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

10. 3. Morphology
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10. T

11. 4. Susceptibility & Resistance
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10. T

12. 5. Cultivation

13. 6. Pathogenesis

14. 7. Symptoms of common cold

15. 8. Laboratory Diagnosis