Image equals 1000 words. Here, ÷ reveal some important points related to safety in virology lab and common techniques are being used as well.

1. Important points in practical
virology
Abdou Mohammad Nagy, DVM, PhD
Department of Virology
Faculty of Veterinary Medicine
Zagazig University, Zagazig 44519, Egypt

2. Biological safety cabinets
Class I biological safety cabinet
http://www.phac-aspc.gc.ca/publicat/lbg-ldmbl-04/ch9-eng.php

3. Class II biological safety cabinet
http://www.phac-aspc.gc.ca/publicat/lbg-ldmbl-04/ch9-eng.php

4. Class III biological safety cabinet
http://www.phac-aspc.gc.ca/publicat/lbg-ldmbl-04/ch9-eng.php

5. Work under BSC

6. Tissue homogenizer Ice box Ice pack
Pestle & mortar

7. What is Positive pressure protective suit (PPPS)?
Fresh, filtered air is typically supplied to
the interior of a PPPS via overhead
tubing. In addition to the physical
barrier provided, the positive
pressurization offers additional
protection in the event of exposure
through a defect or puncture in the suit,
for if the suit’s integrity is compromised,
air will be forced out instead of being
sucked in. Extensive training with the
PPPS is required to safely pursue
research in a PPPS within a biosafety
facility.
https://en.wikipedia.org/wiki/Positive_pre
ssure_personnel_suit

8. Do you want to work with Ebola virus?
http://www.naturalnews.com/047226_Ebola_outbreak_Dallas_heal
th_care_worker.html

9. Could you Identify improper biosafety problems?

10. Common safety practices
https://www.google.com.eg/search?q=biosafety+symbols

11. How do you use fire extinguisher?
http://www.fireonline.com.au/extinguishers

12. Types of fire extinguisher
http://www.fireandsecurity-alarms.co.uk/fire-extinguishers/

13. Liquid nitrogen hazards in virology lab

14. University of Windsor students, overseen by Andrzej Sobiesiak, the head of the mechanical engineering
department, right, transport a tank designed to carry 200 litres of liquid nitrogen along Wyandotte Street
http://windsorstar.com/news/local-news/probe-launched-after-students-roll-hazardous-material-down-
street

15. Evaporated Liquid Nitrogen-Induced Asphyxia: A Case Report
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526498/
Petechiae (arrowheads) were revealed in periorbital areas of the
decedent.

16. What does your lab need to detect
high levels of liquid N2??

17. Virus inoculation in fertile eggs

18. Good vs bad eggs

19. Virus Inoculation in fertile eggs

20. A) Curled and dwarfed embryos post
IBV infection. B) Normal embryo
https://www.researchgate.net/publica
tion/285581786_Phylogentic_analysis
_of_recent_infectious_bronchitis_viru
s_isolates_from_broiler_chicken_farm
s_in_Kafrelsheikh_Egypt/figures?lo=1
Pock lesions
https://en.wikipedia.org/wiki/Chorioallantoic
_membrane#/media/File:Smallpox_CAM.png

21. Mouse inoculation (I like it)
Handling of mice
http://www.usp.br/bioterio/Artigos/Procedimentos%20experimentais/Routeadministration-4.pdf

22. S/C inoculation
http://www.usp.br/bioterio/Artigos/Procedimentos%20experimentais/Routeadministration-4.pdf

23. Intracerebral inoculation
http://www.usp.br/bioterio/Artigos/Procedimentos%20experimentais/Routeadministration-4.pdf

24. Intraperitoneal inoculation Intranasal inoculation
http://www.usp.br/bioterio/Artigos/Procedimentos%20experimentais/Routeadministration-4.pdf

25. Intravenous inoculation

26. Cell culture equipment Inverted microscope
Roux bottle
Leighton culture tube

27. Primary cell culture preparation
http://www.cloud-clone.com/topic/Primary-cell-culture.html

28. Cell subculture, freezing & Thawing
https://www.abmgood.com/marketing/knowledge_base/cell_culture_introduction.php

29. CPE produced by viruses
http://abbs.oxfordjournals.org/content/41/12/998/F2.expansion

30. http://www.virology.ws/2009/05/28/influenza-microneutralization-
assay/

31. What is the difference between two images?

32. Different pH of cell culture medium
Pink YellowRed

33. Thank you