The document describes an experiment where researchers introduced point mutations into the DNA sequence of Mizm1 in a reporter plasmid. They then measured expression levels of the original and mutated plasmids when transfected into cells. The mutations reduced expression levels, suggesting the mutations decreased the ability of Mizm1 to activate transcription of the reporter gene. This is consistent with the mutations disrupting binding of proteins necessary for Mizm1's transcriptional activation function.