You presently have 120000 in an account that earns 6 comp.pdf

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You presently have $120,000 in an account that earns 6% compounded monthly. You will be making month end deposits of $400 in the account for 12 years. You want to have $600,000 in the account at the end of the 12 year term. How much extra will you have to contribute monthly for the last 8 years of the term in order to achieve that goal?.

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You have identified two genes, Parl and Mst5, which you suspect are involved in the embryonic development of the mouse. To study these genes, you isolate total DNA, total RNA, and total protein samples from mice at the following developmental stages: 1) sarly embryos ( 8 days post fertilization, or 8dpf ) 2) late embryos ( 15 days post fertilization, or 15dpf ) 3) newborn pups (NB) 4) 3-month-old adults (A) The expression of Parl and Mst5 during development may be controlled at the RNA level (by regulating either transcription or mRNA stability) or at the protein level (by regulating translation or protein stability). To begin to understand Parl's regulation, you decide to perform Northern and Western blots on your samples. a. Compare and contrast theYou have isolated two different yeast strains, strain 1 and strain 2, each of which fils to grow in the absence of arginine. You want to clone the wild-type copy of the gene or genes that are mutated in strain 1 and strain 2 . To do so you plan to: i. Obtain fragments of the entire yeast genomic DNA ii. Cut the chosen vector and ligate each fragment into a vector iii. Use this pool of original and recombinant vectors to transform E. coll cells iv. Select for E coli cells that have obtained an original or recombinant vectors v. Screen for E. coli transformed with a recombinant plasmid vi. Obtain recombinant plasmids from the library and transform yeast vii. Clone by complementation the gene that can restore the yeast to arginine prototrophy. a. To construct a yeast genomic library in E. coli that will allow you to successfully complete step 7 above, what would be the phenotype of the yeast you would choose as the donor for the genomic DNA? ( 3 marks) b. You choose the vector pBlue, shown below. Note that the cloning site lies within lacZ, the coding region of the gene that encodes -galactosidase. A cell that expresses -galactosidase can take a substrate called X-gal and cleave the -1,6 linkage to form a product that is bright blue. For each of the following sequences found on PB Bue, list the step or steps (1-7 above) for which that sequence is needed and explain the role that sequence plays. (7 marks).

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You have been hired as consultants to research, report and present to a senior management team interested in what is currently happening in the Caribbean market, over the last three years. The senior management team would like guidance based on the two scenarios presented below. This guidance should support their strategic policy decisions over 2-3 years. You are advised to make assumptions where necessary, based on your expertise as consultants in credit analysis and lending. Gateway has a strong history of serving customers as Corporate Investment Managers in St. Lucia, St. Vincent and the Grenadines, St. Lucia and Trinidad. It is interested in strengthening its relationship with Entrepreneurs in these countries by providing lending services. Gateway recognizes the need and demand for financing of Small Medium Enterprises. However, the Gateway management team realized that before it could scale up the capacity of its financial services department and introduce new lending programs, it needed strong credit and lending policies and procedures in place. To date, Gateway has no loan policies or procedures for Small Medium Enterprises. Based on the scenario, in relation to the countries stated: (a) Give an analysis of how the critical requirement, namely, creditworthiness and, asset or collateral can determine the success of small, medium enterprises. (b) (i) Discuss the importance of a business plan (ii) Analyze the likelihood that a small to medium-sized business will be successful in obtaining a credit facility with a thorough business plan and the extent to which the business plan can help lenders assess the likelihood that the small to medium-sized business will be able to repay the credit facility (c Discuss the importance of strengthening Gateways relationship with Entrepreneurs in these countries by providing lending services.

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