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BREEDCAFS (H2020): Development of new BREEDing strategies
for Coffee trees under current and future climates and
adressing sustainability issues (AgroForestry Systems)
At glance:
 full proposal: deposited 09/16 - accepted 12/16, beginning 01/06/2017 (48 months)
 EU Contribution: € 4,200,000.00
 19 participants
 6 Work Packages (WPs)
 VN partners: VAAS, NOMAFSI and AGI
 Kick-off meeting (Montpellier 03-05/07/17)
CIRAD, IRD,
UM, EAF
ISA, NovaID
NUI Galway
UPCH
ILLY
Nicafrance
SNV
MPG
RWTH Aachen
WCR, ASU
Abord
IRAD
CIRAD
(Fr. Guyana)
VAAS,
NOMAFSI, AGI
Objectives:
 Determination of physiological targets for selection of varieties of Coffea arabica
better adapted to AFS
 To provide a toolkit for the identification of metabolic and molecular predictors for
desirable traits to AFS and the prediction of superior parental crosses for
generation of new C. arabica hybrids suited for AFS
 To validate these molecular markers and the new breeding strategy through the
selection of a new wave of C. arabica hybrids before the end of the project
 To investigate the C. arabica F1 hybrids grown in the end-users’ environment, i.e.
within coffee farmers’ fields with their specific local edaphic and climatic
conditions
 To assess the performance for yield, disease resistance and cup quality of
C. arabica F1 hybrids in AFS under different climatic settings (Vietnam, French
Guyana, Cameroon and Nicaragua)
BREEDCAFS (H2020): Development of new BREEDing strategies
for Coffee trees under current and future climates and
adressing sustainability issues (AgroForestry Systems)
 WP1: Understanding responses of F1 hybrids of C. arabica to biotic and abiotic stresses
 Identify robust markers (allelic, molecular, metabolic…) for prediction of best genitors and early
evaluation of F1 hybrids with high-quality and high-yielding under AFS (Partners: ISA, Cirad, Nomafsi)
WP2: Integration and analysis of phenotypic and molecular data via the development of
an interactive bioinformatic platform for assisting coffee breeding
 Evaluate the performance for yield, tolerance to heat and water stresses (Partners: NUIG, Max Planck..)
WP3: On-farm assessment (performance and profitability) (“demoplots”)
 Assess the productivity and profitability of the new Arabica F1 hybrids in AFS (Partners: Nomafsi, IRAD.)
WP4: Ensuring large-scale dissemination of F1 hybrids in the context of AFS through
technological transfers and economic incentives
 Ensure large-scale vegetative propagation of Arabica hybrids by SE (Partners: AGI, NicaFrance, ILLY…)
WP5: Sustainability and scaling-up challenges of the novel breeding strategy
developped in BREEDCAFS
 Develop a scaling-up plan of the novel breeding strategy of BREEDCAFS that ensures dissemination
beyond the project (Partners: UCPH, ICRAF, SVN, NOMAFSI,…)
WP6: Management, communication, dissemination
 Structure, coordinate and monitor the implementation of BREEDCADS by facilitating
collaboration within and between project partners and WPs
BREEDCAFS (H2020): Development of new BREEDing strategies
for Coffee trees under current and future climates and
adressing sustainability issues (AgroForestry Systems)
 WP1: Understanding responses of F1 hybrids of C. arabica to biotic and abiotic stresses
 2018: setting-up trials in “station” (NOMAFSI -Sơn La province)
WP2: Integration and analysis of phenotypic and molecular data via the development of
an interactive bioinformatic platform for assisting coffee breeding
WP3: On-farm assessment (performance and profitability) (“demoplots”)
 2018: setting-up “demoplot” trials with farmers) (NOMAFSI -Sơn La province)
WP4: Ensuring large-scale dissemination of F1 hybrids in the context of AFS through
technological transfers and economic incentives
 vegetative propagation of Arabica hybrids by Somatic Embryogenesis (AGI)
WP5: Sustainability and scaling-up challenges of the novel breeding strategy
developped in BREEDCAFS
WP6: Management, communication, dissemination
BREEDCAFS (H2020): Development of new BREEDing strategies
for Coffee trees under current and future climates and
adressing sustainability issues (AgroForestry Systems)
Involvement of Ph. Vaast & P. Marraccini in BREEDCAFS (2017-2021)
M&M: F1 hybrids of C. arabica + controls (parents) ± agroforestry (AFS) systems
Activities (LMI Rice & Coffee – AGI – Hanoi )
 reception / acclimatization / diffusion of materials (trials)
09/17 10/17-02/18 03/18
 Set-up plant trials in fields:
 WP1 trials in “station” (NOMAFSI)
(Sơn La province)
 WP3 trials for “demoplot” (farmers)
(Sơn La province)
 Start mid-2018:
• eco-physiological measurements (PD, A, gs…)
• phenotyping
• sampling for analyses planned in WP1 / WP3 & WP4:
 molecular analyses: ARN extractions (leaf) for RNAseq (Fr) et qPCR (Vn)
 biochemical analyses: leaves (& fruits?) > Fr
 bean quality (phenological & sensorial) analyses
 WP4 “Large-scale vegetative propagation of Arabica hybrids by SE” (AGI)
BREEDCAFS (H2020): Development of new BREEDing strategies
for Coffee trees under current and future climates and
adressing sustainability issues (AgroForestry Systems)

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BREEDCAFS (H2020): Development of new BREEDing strategies for Coffee trees under current and future climates and addressing sustainability issue (AgroForestry Systems)

  • 1. BREEDCAFS (H2020): Development of new BREEDing strategies for Coffee trees under current and future climates and adressing sustainability issues (AgroForestry Systems) At glance:  full proposal: deposited 09/16 - accepted 12/16, beginning 01/06/2017 (48 months)  EU Contribution: € 4,200,000.00  19 participants  6 Work Packages (WPs)  VN partners: VAAS, NOMAFSI and AGI  Kick-off meeting (Montpellier 03-05/07/17) CIRAD, IRD, UM, EAF ISA, NovaID NUI Galway UPCH ILLY Nicafrance SNV MPG RWTH Aachen WCR, ASU Abord IRAD CIRAD (Fr. Guyana) VAAS, NOMAFSI, AGI
  • 2. Objectives:  Determination of physiological targets for selection of varieties of Coffea arabica better adapted to AFS  To provide a toolkit for the identification of metabolic and molecular predictors for desirable traits to AFS and the prediction of superior parental crosses for generation of new C. arabica hybrids suited for AFS  To validate these molecular markers and the new breeding strategy through the selection of a new wave of C. arabica hybrids before the end of the project  To investigate the C. arabica F1 hybrids grown in the end-users’ environment, i.e. within coffee farmers’ fields with their specific local edaphic and climatic conditions  To assess the performance for yield, disease resistance and cup quality of C. arabica F1 hybrids in AFS under different climatic settings (Vietnam, French Guyana, Cameroon and Nicaragua) BREEDCAFS (H2020): Development of new BREEDing strategies for Coffee trees under current and future climates and adressing sustainability issues (AgroForestry Systems)
  • 3.  WP1: Understanding responses of F1 hybrids of C. arabica to biotic and abiotic stresses  Identify robust markers (allelic, molecular, metabolic…) for prediction of best genitors and early evaluation of F1 hybrids with high-quality and high-yielding under AFS (Partners: ISA, Cirad, Nomafsi) WP2: Integration and analysis of phenotypic and molecular data via the development of an interactive bioinformatic platform for assisting coffee breeding  Evaluate the performance for yield, tolerance to heat and water stresses (Partners: NUIG, Max Planck..) WP3: On-farm assessment (performance and profitability) (“demoplots”)  Assess the productivity and profitability of the new Arabica F1 hybrids in AFS (Partners: Nomafsi, IRAD.) WP4: Ensuring large-scale dissemination of F1 hybrids in the context of AFS through technological transfers and economic incentives  Ensure large-scale vegetative propagation of Arabica hybrids by SE (Partners: AGI, NicaFrance, ILLY…) WP5: Sustainability and scaling-up challenges of the novel breeding strategy developped in BREEDCAFS  Develop a scaling-up plan of the novel breeding strategy of BREEDCAFS that ensures dissemination beyond the project (Partners: UCPH, ICRAF, SVN, NOMAFSI,…) WP6: Management, communication, dissemination  Structure, coordinate and monitor the implementation of BREEDCADS by facilitating collaboration within and between project partners and WPs BREEDCAFS (H2020): Development of new BREEDing strategies for Coffee trees under current and future climates and adressing sustainability issues (AgroForestry Systems)
  • 4.  WP1: Understanding responses of F1 hybrids of C. arabica to biotic and abiotic stresses  2018: setting-up trials in “station” (NOMAFSI -Sơn La province) WP2: Integration and analysis of phenotypic and molecular data via the development of an interactive bioinformatic platform for assisting coffee breeding WP3: On-farm assessment (performance and profitability) (“demoplots”)  2018: setting-up “demoplot” trials with farmers) (NOMAFSI -Sơn La province) WP4: Ensuring large-scale dissemination of F1 hybrids in the context of AFS through technological transfers and economic incentives  vegetative propagation of Arabica hybrids by Somatic Embryogenesis (AGI) WP5: Sustainability and scaling-up challenges of the novel breeding strategy developped in BREEDCAFS WP6: Management, communication, dissemination BREEDCAFS (H2020): Development of new BREEDing strategies for Coffee trees under current and future climates and adressing sustainability issues (AgroForestry Systems) Involvement of Ph. Vaast & P. Marraccini in BREEDCAFS (2017-2021)
  • 5. M&M: F1 hybrids of C. arabica + controls (parents) ± agroforestry (AFS) systems Activities (LMI Rice & Coffee – AGI – Hanoi )  reception / acclimatization / diffusion of materials (trials) 09/17 10/17-02/18 03/18  Set-up plant trials in fields:  WP1 trials in “station” (NOMAFSI) (Sơn La province)  WP3 trials for “demoplot” (farmers) (Sơn La province)  Start mid-2018: • eco-physiological measurements (PD, A, gs…) • phenotyping • sampling for analyses planned in WP1 / WP3 & WP4:  molecular analyses: ARN extractions (leaf) for RNAseq (Fr) et qPCR (Vn)  biochemical analyses: leaves (& fruits?) > Fr  bean quality (phenological & sensorial) analyses  WP4 “Large-scale vegetative propagation of Arabica hybrids by SE” (AGI) BREEDCAFS (H2020): Development of new BREEDing strategies for Coffee trees under current and future climates and adressing sustainability issues (AgroForestry Systems)