Tangential Flow Filtration [TFF] is used for clarifying, concentrating and purifying proteins. Filtration is a pressure driven separation process that uses a membrane to separate components in liquid solution or suspension based on size and charge difference. Types of Membrane Filtration 1. Normal Flow Filtration - Dead End Filtration 2. Tangential Flow Filtration - Cross Flow Filtration

1. Tangential Flow Filtration: Membrane
Types and Process Parameters
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2. • Tangential Flow Filtration [TFF] is used for
clarifying, concentrating and purifying proteins.
• Filtration is a pressure driven separation process
that uses a membrane to separate components in
liquid solution or suspension based on size and
charge difference.

3. Types of Membranes
•
1. Normal Flow Filtration - Dead End Filtration
• 2. Tangential Flow Filtration - Cross Flow
Filtration

4. Types of TFF
• 1. Micro Filtration
• 2. Ultra Filtration
Ultra Filtration is catagorized into two
• Virus Filtration
• High Performance Tangential Flow Filtration

5. Process Goals
• Final Product Concentration
• Feed Volume Reduction
• Extent of Buffer Exchange
• Contaminant Removal Specification

6. Primary Protein Processing Goals
• High Product Yield
• High Product Quality
• High Product Purity
• Controlled Bioburden

7. TFF Membranes
• Polyethersulfone (PES)
• Regenerated Cellulose

8. Types of TFF Membranes
• Flat Plate
• Spiral Wound
• Hollow Fiber

9. Key Parameters to Optimize
• Cross Flow Rate
• Transmembrane Pressure
• Filtrate Control
• Membrane Area
• Diafiltration Design

10. Factors affecting Product Yield
These are the four contribution of product loss
during Tangential Flow Filtration
• Retention Loss
• Adsorption Loss
• Solubility Loss
• Unrecoverable hold up volume loss

11. Factors affecting Product Quality
Quality of product is compromised due to
aggregation or denaturation caused by
• Micro cavitation
• Air-Liquid Interface
• High Protein Concentration
• Temperature Effects

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Flow Filtration
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