Calculation 4 We monitor reaction rates of the enzyme from .pdf

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Calculation #4 We monitor reaction rates of the enzyme from Calculation #3, but now using a different substrate (Substrate B). Testing Substrate B at [S]1=5mM and [S]2=10mM, the measured rates are v1=50 mM/min,v2=60mM/min. Provide a first estimate of the Km and Vmax values of the enzyme for Substrate B based on these data! (Compare them to those for Substrate A! ) [Note, that due to experimental errors precise estimation of Km and Vmax in practice requires measurement of velocities at several different substrate concentrations. However, theoretically, these parameters are defined already by two [S]-v pairs.].

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