1. Medical Microbiology
HS0804

2.  Purpose: induction of an immune response to
achieve protection against an antigen to protect
a future challenge or to initiate a reaction
against already present antigent
 Method: Injection or oral uptake( e.g. Protein,
toxin, attenuated pathogen etc.) together with
adjuvant or carrier

3.  DNA vaccines are made of a modified form of
an infectious organism's DNA
 microbe DNA( not human) is inserted into
plasmid vector
 when injected into a person(intramuscular
injection, intradermal saline injection) -leads to
the expression of the genes within this
modified, foreign DNA
 result, the person's immune system responds in
both antibody and cell mediated

4.  when injected into a person -leads to the
expression of the genes within this modified,
foreign DNA
 result, the person's immune system responds in
a protective manner
 Human cells take up some plasmids and
express microbial DNA in the form of protein

5.  Gene gun: initiates responses by transfected or
antigen bearing epidermal Langerhans cells
that move in lymph from bombarded skin to
the draining lymph nodes
 Intramuscular injection: the functional DNA
appears to move as free through blood to
spleen where professional APC initiate
responses