Student exercise -mix and match stages of gel electrophoresis.

1. Gel Electrophoresis
Gel electrophoresis is a way of visualizing how DNA has been cut up by separating DNA fragments according to their size.
The process uses some basic properties of DNA and of the gel setup.
Match the five bullet pointed statements with a reason from the second column and an outcome from the third column.
Rewrite the sentences
because they can conduct electricity so DNA will move on the
The porous agarose gel is
agarose gel
used.
Because they move through the pores so DNA will move on the
Buffer solutions containing
less easily agarose gel
ions are used.
because they move through the pores so they travel the shortest
DNA is negatively charged
more easily. distance.
because it allows the DNA to move so DNA will move towards a
Smaller fragments move
through the pores. positive electrode
faster
because of the phosphate groups in the so they travel the furthest
Larger fragments move more
backbone
slowly