This bacterial studies are under research. It will much useful for agricultural industry. One can clearly understand the role of wolbachia in the arthropods and also how they reduce the population of such arthropods through cytoplasmic incompatibility.

1. Wolbachia – The biology of
cytoplasmic incompatibility
By,
B.C. Muthubharathi,
III B.Sc.,Biotechnology.

2. What is Wolbachia?
Systematic classification
Kingdom : Bacteria
Phylum : Proteobacteria
Class : Alphaproteobacteria
Order : Rickettsiales
Family : Rickettsiaceae
Genus : Wolbachia
Species : melophagi
persica
pipientis
Infectsarthropod(insects), Nematodes
Parasitic microbe
1924 , Marshall hertig
Biocontrol agent
Biopesticide

3. Is it an Insecticide?
Intracellular
Infect reproductive tissue
Parthenogenesis
Feminization
Male Killing
Cytoplasmic Incompatibility
Alter the Phenotype
It affects
• Cherry Fruit Fly,
• Mosquitoes,
• Mites, Isopods etc.,

4. Cytoplasmic Incompatibility
• Results in sperm and egg unable to form viable
offspring
• Changes in gamete cells
• Leads to delayed entry of male pronucleus
• Embryonic mortality

5. Cellular Mechanism
28 Strains are identified
WMelPop-CLA : Reduce life span of adult
WMel : Induce reproductive phenotype
Modification - Spermatogenisis
Rescue - After fertilzation
Spematocytes and spermatides
Somatic cyst cell surrounding germcell
Prevent transmission of arbovirus , Human parasites
Protect infected insects from RNA virus

6. Role of Wolbachia in Reproduction of insects

7. Areas affected with Dengu

8. Cont..
Unidirectional CI: Mating of infected males and uninfected females results in
CI. All other crosses are compatible.
Bidirectional CI: Mating of males infected with Wolbachia strain I and females
infected by Wolbachia strain II (and vice versa) results in CI.