Use of nano particles in drug delivery.

1. Use of Nanoparticles for drug
Delivery

2.  Nanoparticle – any particle that is sized
between 1 and 100 nanometers (in terms of
diameter)
 The use of nanoparticles
allows one to change the
pharmacokinetic properties
of the drug without
changing the active
compound

3.  Nanomedicine is the medical application of
nanotechnology.

4.  Liposomes
 Polymeric nanoparticles
 Dendrimers
 Fullerenes
 Quantum dots
 Metal nanoparticles
 Magnetic nanoparticles

6.  In recent years, biodegradable polymeric
nanoparticles have attracted considerable
attention as potential drug delivery devices in
view of their applications in drug targeting to
particular organs/tissues, as carriers of DNA
in gene therapy, and in their ability to deliver
proteins, peptides and genes
through a per oral route of administration

7.  Approved by the FDA in 1996 for the treatment of
multiple sclerosis (T-cell therapy)
 Synthetic polymer of amino amino acids (Cop1 composed
of L-Ala, L-Lys, L-Glu, and L-Tyr)
 Administered subcutaneously
 Marketed byTEVA pharmaceuticals

8.  Cop 1 polymer related to myelin binding protein
(MBP)
 Binding to MHC leads to the activation ofT-
suppressor cells
 Competes with several myelin-associated
antigens to bind to MHC class II molecules
 Low toxicity; however, copaxone can only slow
the progression of the disease and reduce the
relapse rate

9.  Gold nanoparticles
 Non-metals used :
 Carbon60

10.  Gold nanoparticles can be novel nanocarriers of peptides and
proteins of interest. Cationic tetraalkylammonium-functionalized
GNPs recognize the surface of an anionic protein through
complementary electrostatic interaction and inhibit its activity
(Verma et al., 2004).
 The activity was recovered due to the release of free protein by
treating the protein–particle complex with glutathione, showing
GNPs as potential protein transporters.
 Pokharkar and coworkers have demonstrated functionalized gold
nanoparticles as the carriers of insulin (Bhumkar and Joshi, 2007).
Chitosan-coated particles strongly adsorb insulin on their surface,
and are effective for transmucosal delivery of insulin.


11.  Recent work has shown that the combination of
phototherapy with conventional gene therapy
offers a high possibility to improve the efficiency
of gene delivery into cells (Mariko et al., 2005).
For example, Niidome et al., (2006) have
investigated the release of plasmid DNA from
spherical gold nanoparticles after exposure to
pulsed laser irradiation.


12.  Interference of gene expression can also be
performed using gold nanorods. Lee et al. have
demonstrated the idea of a remote optical
switch for localized and selective control of gene
interference.
 Thiol-modified sense oligonucleotides were
conjugated with gold nanorods.Thereafter, the
antisense oligonucleotides were hybridized to
the sense oligonucleotides.

13.  After laser irradiation, the double strands of the
oligonucleotides were denatured and the antisense
oligonucleotides were released from the complex
structure.
 These antisense oligonucleotides can bind to the
corresponding mRNA while the sense oligonucleotides
were still attached to gold nanorods through thiol bonds.
Once the mRNA/oligodeoxynucleotide heteroduplex is
formed, its structure will be recognized and degraded by
RNaseH enzymes inside the cell, thereby inhibiting the
normal genetic function of the mRNA (Lee et al., 2009).


14.  Electroporation is another external stimulus
that can be used to release genes from a gold
nanoparticle. Kawano et al. (2006) have
investigated gene delivery in vivo using gold
nanoparticles excited with electrical pulses.
 In this study, gold nanoparticles were
modified with mPEG-SH5000 and conjugated
with plasmid DNA.

15.  These were then injected into anesthetized mice. After a
suitable delay to allow the conjugates to spread in the
mouse, electrical pulses were then applied to the left lobe of
its liver.
 The result was that gene expression was detected in the
major mouse organs. In contrast, the injection of naked DNA
resulted in a 10-fold lower level of detection.
 The degradation of DNA in blood, which occurs in times as
short as 5 min, is evidently the reason for the inefficient
transfection in the latter case.This study illustrates yet
another interesting approach to improve gene delivery using
gold nanoparticles.


17. Carbon nanotubes (CNTs), discovered by
Japanese scientist Iijima in 1991 [1], are now
considered to be a top class subject in
academic researches as well as in various
industrial areas

18.  CNTs can be used as drug carriers to treat tumors .The efficacy of
anticancer drugs used alone is restrained not only by their systemic
toxicity and narrow therapeutic window but also by drug resistance and
limited cellular penetration.
 Because CNTs can easily across the cytoplasmic membrane and nuclear
membrane, anticancer drug transported by this vehicle will be liberated
in situ with intact concentration and consequently, its action in the
tumor cell will be higher than that administered alone by traditional
therapy.
 Thus, the development of efficient delivery systems with the ability to
enhance cellular uptake of existing potent drugs is needed.The high
aspect ratio of CNTs offers great advantages over the existing delivery
vectors, because the high surface area provides multiple attachment

19.  Many anticancer drugs have been conjugated
with functionalizedCNTs and successfully
tested in vitro and in vivo such as epirubicin,
doxorubicin, cisplatin, methotrexate,
quercetin, and paclitaxel .

20.  For drug delivery, most of the sites are accessible
through either microcirculation by blood capillaries
or pores present at various surfaces and
membranes. Most of the apertures, openings, and
gates at cellular or subcellular levels are of
nanometer size
 Hence, nanoparticles are the most suited to reach
the subcellular level.

21.  For any moiety to remain in the vasculature, it needs to have its
one dimension narrower than the cross-sectional diameter of the
narrowest capillaries, which is about 2000 nm.
 Actually, for efficient transport the nanoparticle should be smaller
than 300 nm. But, just moving in the vessels does not serve the
drug delivery purpose. The delivery system must reach the site at
the destination level. This requires crossing of the blood capillary
wall to reach the extracellular fluid of the tissue and then again
crossing of other cells, if they are in the way, and entering the
target cell.These are the major barriers in the transit

22.  A nanoparticle has to do a lot during this sojourn of the carrier
through the vessels (capillaries) and across the barriers.
 There are two routes for crossing the blood capillaries and other
cell layers, i.e., transcellular and paracellular.
 In the transcellular route, the particulate system has to enter the
cell from one side and exit the cell from the other side to reach the
tissue. The particulate system has to survive the intracellular
environment to reach the target tissue.
 The other route is paracellular. In this, the particlulate system is
not required to enter the cell; instead, it moves between the cells.
These intercellular areas are known as the junctions. Passing
through the junctions would obviate destruction of the carrier by
the cell system.

23.  Paracellular movement of moieties including ions, larger
molecules, and leukocytes is controlled by the cytoskeletal
association of tight junctions and the adherence junctions called
apical junction complex.
 While tight junctions act as a regulated barrier, the adherence
junctions are responsible for the development and stabilization of
the tight junctions. Different epithelial and endothelial barriers
have different permeabilities mainly because of the differences in
the structure and the presence of tight junctions. While epithelia
and brain capillary endothelium exhibit a high degree of barrier
function, the vascular endothelium in other tissues has greater
permeability. The tight junctions control the paracellular
transport. For example, diffusion of large molecules may not be
feasible, but migration of white cells is allowed

24.  As the nanoparticle based drug delivery is achieved by particle transport,
it is important to understand the blood flow rates and volumes of various
organs and tissues. Considering the body’s distribution network, the
blood vascular system, the body could be divided into several
compartments based on the distributional sequencing and
differentiation by the blood vascular system.