19. Day 2 E.coli Transformation with pGLO plasmid Ampicillin resistance gene (Amp r ) and target gene on bacterial plasmid Bacterial clones Cell division Transformation mixture is plated on to agar plate containing Ampicillin Only E. coli containing plasmid survive on Ampicillin plates
20. Results of Inserting Foreign DNA into an Organism Cells will multiply and produce desired gene product pGlO gene expression vector: Green Fluorescent Protein
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23. Isolation and Purification of Green Fluorescent Protein Transformed cells #1 #2 #3 Fraction number Test tubes Courtesy of Bio-Rad
33. PAGE of pGLO samples after IEX chromatography A B C D E F G H I J A = molecular ladder B = no sample C = IEX #1 fraction column D = I EX # 2 fraction column E -G = IEX wash fractions H = Supernatant I = molecular ladder J = standard GFP 29,000 mol wt 29,000 mol wt
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36. $ Protein is Cash $ Local Teachers Workshops Biomanonline.org
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Notes de l'éditeur
Here is the premise of the ‘Protein is Cash’ Workshops designed to grow the local biotech/biomanufacturing network across the nation.
Jim to tell NVC what our vision and mission statements are: Vision : To be the nationally-recognized center of excellence that develops a world-class sustainable biomanufacturing workforce to improve the quality of life. Mission: Coordinate local and regional efforts into a national biomanufacturing education and training system to promote, create, and sustain a qualified workforce.
![Protein is Cash:State of the Art Curriculum to Support the Bioeconomy Mary Jane Kurtz, Ph.D NBC 2 Consultant [email_address]](https://image.slidesharecdn.com/kurtzbiomanufacturing-110721155820-phpapp02/85/Kurtz-biomanufacturing-1-320.jpg)
![Basis of the Bioeconomy ,[object Object],[object Object],[object Object]](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)