This videos helps to know about tissue culture.

1. Tissue Culture

2. What is Tissue Culture ?
• Refers to technique of
growing plant cells,
tissues, organs, seedsor
other plant parts in a
sterile environment on a
nutrient medium

3. Media
• must be sterile
• autoclave at 250 F at 15 psi
for 15 minutes
• Murashige and Skoog
media is commomly used

4. Explant
• portion of plant removed
and used for T.C.

5. Patterns of
multiplication
• stage II - light 100-300 foot
candles
• callus - shoots - roots
• stage III - rooting - light
intensity 1000-3000 foot
candles

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