it is a software which helps the molecular biologist nd biotechnologist to plan their experimnets.

1. SNAPGENE
VIEW RICHLY ANNOTATED SEQUENCE FILES
Manish Thakur
Systems Biology and
Bioinformatics
M.Sc-1

2. OUTLINE
 INTRODUCTION
WHAT SNAPGENE DO
• VISUALIZE
• ANNOTATE
• PLAN & DOCUMENT
 STRATEGIES FOLLOWED
 OUTPUT
 CONCLUSIONS

3. SnapGene offers the fastest and easiest way to plan,
visualize, and document your molecular biology
procedures. The streamlined interface supports a
range of cloning and PCR manipulations.
SnapGene was developed by GSL Biotech Chicago.
First version of SnapGene was 0.2 released on Apr 3,
2008.
Latest version available SnapGene 2.7.1 on Mar 6,
2014.
 Buy/Free trial of the software from the SnapGene
website www.snapgene.com.
INTRODUCTION

4. SYSTEM REQUIREMENTS
O.S Windows 8, 7, Vista or XP
Mac OS X 10.7 or later
Memory 1 GB RAM
Hard Disk 250 MB available disk space
Display 1024 x 768 or higher resolution
Written in Visual Studio, C++

5. • Create a DNA sequence file.
• Browse or print a DNA sequence and its annotations
using customizable Map, Sequence, Enzymes, Features,
Primers, and History views.
• Search a DNA sequence to match either a DNA
query, or a protein translation, or an annotation.
• Open and edit sequence trace files, and align them
to a reference sequence.
VISUALIZE

8. •Automatically annotate common features,
or manually annotate coding sequences and
other features.
•Design and annotate primers for PCR,
sequencing, or mutagenesis.
•Identify open reading frames (ORFs) with a
single mouse click.
ANNOTATE

12. • Simulate restriction cloning and other types of
cloning using a rich intuitive interface.
•Simulate conventional or overlap extension PCR.
•Simulate primer-directed mutagenesis.
•Simulate agarose gel electrophoresis.
•Record all of your manipulations in an
automatically generated graphical cloning history.
PLAN AND DOCUMENT

17. What we can do with SnapGene
o Restriction Cloning
o Primers & PCR
o Mutagenesis
o Gibson Assembly
o In-Fusion Cloning
o Gateway Cloning
o TA or GC cloning

18. Restriction Cloning
Cloning by restriction enzyme digestion and
ligation is a simple and easy way of moving a
fragment of double stranded DNA from one
plasmid to another.

29. PRIMER DESIGNING & PCR
Amplify a single copy or a few copies of a
piece of DNA.

39. PCR
Machine
Restriction
Cloning

42. SnapGene has a specific purpose and it is highly
flexible as far as finding items or groups of similar
items. Its applicability is restricted to the field of
molecular biology.
I perform restriction cloning ,Primers & PCR strategy
under SnapGene
It is an indispensable tool for building plasmid maps of
our various recombinant constructs to aid with the
cloning process. It is a user-friendly & time saver
software for RDT projects.
CONCLUSION

43. oEvery DNA construct in a lab will be fully
documented.
oPublications will include complete
annotated sequences and histories of
every DNA construct used.
oTime and money will be saved, and
biomedical research will move faster.
Vision of the Future with
SnapGene