Enzo社腎臓損傷＆腎毒性研究用試薬、マウス尿中アルブミン定量ELISAキット Albuwell M、Cell Biolabs社 腎機能アッセイ商品類、肝細胞培養キット（ラット・マウス・ハムスター）、小型肝細胞培養キット（ラット）…等をご紹介。

4. ®

5. Plasma
Serum
1 to 2 1 to 4 1 to 8
Plasma and Serum Dilutions
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
OD630nm

8. 2.5
2
1.5
1
0.5
0
OD540nm
0 500 1000 1500 2000 2500 3000
Lipid Standard (mg/dL)

9. 0
200
400
600
800
1000
RelativeRatio
Exposure Time
VAC01
0
2
4
6
8
10
12
RelativeRatio
Exposure Time
BAT10
NE(-) 1H 3H 6H 24H NE(-) 1H 3H 6H 24H
kDa
34
VAC01 VAC01
+
NE6h
VAC01
+
NE24h
BAT10
UCP1
43
GAPDH
26

10. 2500
2000
1500
1000
500
0
RFU
100 20 4 0
ヒト血清（％）
ヒト血清 (µL) ヒト血漿 (µL)
50 12.5 3.125 0 50 12.5 3.125 0
2.4
2.2
2
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
OD450nm

13. Mouse Preadipocyte 3T3-L1
Medium：D-MEM + 10%CS, D-MEM +10%FBS
Cell collection2DG (1 mM)Insulin (1μM) Phloretin (200μM)
30min 48min 50min 70min
serumfree medium
(D-MEM)
HKRP buffer
0-6hr
2DG - + + +
Insulin - - + -
Phloretin*1 - - - +
3000
2000
1000
2500
1500
500
0
2DG6Pincorporated
intocells(pmole/well)
*1 Phloretin : glucose
transporter inhibitor
serumfree medium
(DE/F-12)
HKRP buffer
Wortmannin (50 nM) 2DG (1 mM)Insulin (1μM) Phloretin (200μM)
0 30min 48min 50min 70min
Cell collection
(DE/F-12)
-6hr
Human Preadipocyte －subcutaneous fat tissue
（ScienCell Research Laboratories Cat.# 7220）
Medium
：Visceral Adipocyte Culture Medium ver.2
（Cat.# PMC-VACM2-COS）
2DG - + + + +
Insulin - - + + +
Phloretin - - - + -
Wortmannin - - - - +
2DG6Pincorporatedintocells
(pmole/µgDNA)
800
600
400
200
0
*1
Phloretin
: glucose transporter
inhibitor
*2
Wortmannin
: PI3 kinase inhibitor
実験例 2 ヒト皮下脂肪細胞による 2DG の取り込み
y = 407x + 4502.6
R² = 1
0
5000
10000
15000
20000
25000
30000
0 10 20 30 40 50
FluorescenceIntensity
2DG6P(μM)
0
10
20
30
40
A B C
2DG6Pincorporatedintocells
(nmole/12wellculturedish)
添加物 Sample A Sample B Sample C
インスリン － － ＋
2DG － ＋ ＋

20. Denaturing extraction Native extraction
250
98
64
50
36
30
16
M M
S.cereviseae
L.Lactis(Gram
＋
)
S.cereviseae
filam
entus
fungus
filam
entus
fungus
250 350 450 550 650
Wavelength (nm)
Excitaion
Emission

21. Live-or-Dye 350/448 Live-or-Dye 405/452 Live-or-Dye 405/545 Live-or-Dye 488/515
Live-or-Dye 568/583 Live-or-Dye 594/614 Live-or-Dye 640/662 Live-or-Dye 750/777
Live-or-Dye 488/515 Live-or-Dye 568/583
Live-or-Dye 594/614 Live-or-Dye 640/662

22. 0
50
100
150
0 5 10 15
Albuminproduction(µg/day/well)
Culture time (day)
Plastic Plate/5%-O2
VECELL® G-Plate/5%-O2
Plastic Plate/21%-O2
VECELL® G-Plate/21%-O2
Plastic Plate/5→21%-O2
VECELL® G-Plate/5→21%-O2
3
15
12
9
6
3
0
6 10
Resorufin(pmol/h/well)
Culture time (Day)
Plastic Plate/O2
-
VECELL® H-Plate/O2
+

23. ScienCellResearchLaboratories
TM
®

24. 1
2
3
4
5
6 7

26. サンプル希釈液
マウス血清
マウス血漿
組織培養培地
他社製品
マウス IFN-β (pg/㎖)
0.1 1 10 100 1000
吸光度【450nm】
3
2
1
0
pg/㎖
OD450nm
5
0.5
0.05
0.5 5 50
Mammalian Mu Beta
E.coli Mu Beta