Retroviruses are negative single-stranded RNA viruses in which tRNA serves as a primer for the mRNA synthesis. The mRNA is then reverse transcribed into DNA, which is then integrated into the chromosome at a multiplicity of sites, particularly those that are transcriptionally active. Retroviral vector-mediated gene transfer has been central to the development of gene therapy. 

1. Custom Retrovirus
Production
Presented by Creative Biogene
About Creative Biogene
Creative Biogene provides kits, reagents, and services that help researchers
explore questions about gene discovery, regulation, and function. Creative
Biogene holds a leadership position in the global market and is committed to
improving the human condition through biotechnology.

2. Introduction
• Retroviruses are negative single-stranded RNA
viruses in which tRNA serves as a primer for
the mRNA synthesis. The mRNA is then
reverse transcribed into DNA, which is then
integrated into the chromosome at a
multiplicity of sites, particularly those that are
transcriptionally active. Retroviral vector-mediated
gene transfer has been central to the
development of gene therapy.

3. Advantages
• Retroviruses have several distinct advantages
over other vectors:
• transform their single-stranded RNA genome
into a double stranded DNA molecule that
stably integrates into the target cell genome.

4. Provider
• Creative Biogene is pioneering in the
development of retroviral technology with years
of experience in retroviral services. Creative
Biogene's custom retrovirus production is a
highly efficient approach to deliver genes of
interest for stable expression into a broad range
of dividing mammalian cells and it aims to
provide valuable research tools and services in
molecular biology.

5. Custom Retrovirus Construction
Services
 Construction of ordered retrovirus vector (gene expression and
shRNA expression).
 Production of a retrovirus producer cell line by transfection.
 Production of viral supernatant by transient transfection as
well as assay for replication competent
retrovirus.
 Quality control assays including quantification of genome
copies and quantification of retroviral
vector infectivity.

6. Contact
Creative Biogene at
http://www.creative-biogene.com/Services/Custom-Tel: 1-631-626-9181 or 44-207-048-3343
Email: info@creative-biogene.com

7. Contact
Creative Biogene at
http://www.creative-biogene.com/Services/Custom-Tel: 1-631-626-9181 or 44-207-048-3343
Email: info@creative-biogene.com