A talk outlining the basics of method development using a tandem mass spectrometer

1. Implmentation of TMS at BHH

3. General Principles

5. General Principles

7. Interface

8. General Principles

11. ESI Soft ionisation technique; little fragmentation Large molecules may be protonated more than once Peaks seen for same compound with different m/z ratios

16. General Principles

19. Mass Separation

20. General Principles

22. Electron Multiplier

24. TMS

31. MRM

32. TMS

45. Adducts and Clusters Formed in LC-MS Cluster/Adduct Source of Cluster Occurence Mass of Cluster Ion [M + CH3COO]- Acetic Acid APCI & IS -ve mode M + 59 [M + Cl]- Chlorinated Solvent APCI & IS -ve mode M + 35 [M + NH4]+ Ammonia APCI & IS +ve mode M + 18 [M + Na]+ Sodium Salts APCI & IS +ve mode M + 23 [M+K]+ Potassium Salts APCI & IS +ve mode M + 39 [M + CH3CNH]+ Acetonitrile APCI & IS +ve mode M + 42 [M+CH3OHH]+ Methanol APCI & IS +ve mode M + 33 [M+H3O]+ Water APCI & IS +ve mode M + 19

55. 152.1 300.2 Codeine 153 293.1 171.3 293.1 Benzoylcgonine-D3 181.2 292.2 152.1 292.2 Morphine-D6 150.1 290.1 168.1 290.1 Benzoylcgonine 198.2 290.1 154.1 290.1 Diazepam-D5 165.2 286.1 152.1 286.1 Morphine 192.9 285.1 154.1 285.1 Diazepam 180.2 282.2 236.2 282.2 Nitrazepam 249.1 278.2 234 278.2 EDDP 208.3 271.1 165.2 271.1 Nordiazepam 224.2 252.1 121.2 252.1 7-aminonitrazepam 119 150.1 91 150.1 Metamphetamine 66.9 142.2 93.1 142.2 125.1 142.2 Amphetamine-D6 119.2 136 91 136 Amphetamine Q3 Q1 Drug

56. Urine top std

57. Accuracy

61. Testosterone by LC-MS/MS (ESI)