05/11 seminar ppt

1. Identification of Genes required for Cytoplasmic Localization in Early C. elegans Embryos Kenneth J. Kemphues James R. Priess Diane G. Morton Niansheng Cheng 皓宇。宇瑄。銘崧

10. Principles of Development (Lewis Wholper) Chapter 5

16. Aim-1 Identifying the genes

21. Genes

22. Aim 2 Observing the defects

23. Equal First Cleavage

26. Table-2 Relative Sizes of AB Blastomeres 37 57±3 par-4(it33) 21 52±1 par-3(e2074) 35 51±2 par-2(it5) 39 53±2 par-1(b274) 57 57±2 N2(wild type) No. of embryos AB/Total Genotype

27. Percent egg length

28. Altered Second Cleavages

29. Altered Second Cleavages

30. Altered Second Cleavages

32. Abnormal Localization of P Granule

34. Wild type par-1 mutant par-2 mutant par-3 mutant par-3 mutant par-4 mutant

37. Abnormal Differentiated Cells Production

42. Grandchildless Phenotype

44. Wild type hermaphrodite par -3 mutant hermaphrodite O ： oocyte S ： spermatheca E ： embryos V ： vulva I ： intestine GS ： somatically derived gonad sheath http://www.wormatlas.org/handbook/fig.s/ReprodFIG1.jpg