Development of technique to make use of orange peels. Production of pectin from orange peel in an innovative method

1. High Value Pharmaceutical Raw
Material from Food Processing Waste

2. Egyptian Pharmaceutical Industry
http://www.oxfordbusinessgroup.com/economic_updates/egypt-regulating-pharmaceuticals-production
Size 120 licensed plants (production: $ 6.6 bn)
(growth rate  11% through 2015)
Raw materials (85% imported)
- Natural
- Synthetic
- sustainable at a comparatively reasonable price
- safeguards purity, health and safety
- protects the environment

3. Orange Production in Egypt
-Production:
-Fresh orange (3.5 million MT in 2012)
 ranked # 6 in the world
-Export:
-Fresh orange (> one million MT in 2012)
 ranked #4 in the world
-Orange Juice (3000 ton in 2012)
 ranked #19 in the world)
-Domestic use  > 2 million MT in 2012
-Waste “Orange peel”  >40% of orange wt.
Orange peel  contain > 20% pectin
- Pectin production in Egypt  ZERO

4. Sources of Pectin
Levels of pectin in plants (fresh weight)
Citrus peels
Oranges
Apples
Carrots
Apricots
Cherries
>20.0%
0.5–3.5%
1.0–1.5%
1.4%
1.0%
0.4%

6. Citrus fruits are rich source of pectin
Peel contains > 20% pectin
Pectin (powdered form)

7. Top ten Countries in Production of Orange in 2011
(million ton)
Rank Country Production Rank Country
Production
1
Brazil
19.8
6
Egypt
3.5
2
USA
8
7
Spain
2.8
3
China
5.8
8
Italy
2.4
4
India
4.5
9
Indonesia 1.8
5
Mexico
4
10
Turkey
1.7
http://faostat.fao.org/site/339/default.aspx

8. Pectin peel : Dried Orange Peel
In Egypt
(US$10 per ton of raw peel)
(US$ 300-600 per ton) in 2011
(US$1,000 per ton) in 2012

9. Pectin value exported in 2012 (US$ 11,212/ton)
World  US$ > 427
Germany 
Czech republic 
Brazil
China 
Egypt 
million
38,102 ton
14,184 ton
3,987 ton
4,191 ton
3,445 ton
0
Pectin value imported in 2012 (US$ 12,000/ton)
Egypt 
US$ 4,38 million
365 ton

10. Pectin
Environmentally-friendly, safe food additive (E440, GRAS)
Application of Pectin
- Pharmaceutical industry
- Food industry, etc..
gelling agents,
Jams
viscosity builders,
Yogurt
Stabilizers,
Juices
protective colloids
-Controlled release formulations
-Carrier for colon-specific drug delivery
- Medical &OTC, personal care, air care
-Nutraceuticals; as dietary fiber (anti-diabetic,
reduce cholesterol, anti-diarrheal)
-wound healing, medical adhesive, demulcent
(throat lozenges), spa products, etc..

12. Objective
Wide Objective
 to produce high value Pharmaceutical raw
material from food processing waste material
in compliance with international standards.
Specific Objective
 to prepare a feasible and profitable
industrial prototype scale unit for the production
of:
a)-Pharmaceutical grade Pectin from orange
peels (as a primary target),
b)-Food grade pectin (as a secondary target)

13. Implementation levels
(I)- Lab scale level for extraction of pectin using:
1)- conventional method
 boiling with H2O, pH 1-2 / 2-3 hr
2)- ultrasound-assisted extraction
 with hot H2O, pH 1.6, 37 min, 395 W
3)- Industry guided extraction method
3 (II)- Industrial pilot-scale level the method
that gives the best extraction yield, quality
and economic feasibility will be applied using
an
industrial pilot-scale unit

14. Pectin testing “for characterization and standardization”
D-galacturonic acid units (GalA)
The tests will be carried out according to FAO manual and will
include; test for pectin, test for amide group, purity, loss on drying,
residual solvents, acid insoluble ash, nitrogen content and
galactouronic acid content, etc..

15. Project Management
Prof. Dr. Meselhy Ragab Meselhy:
Project PI (FOCU, Academic applicant):
 Managing the whole project, leading the phyto. activity
Dr. Amr M. Helal:
CO PI (ITM- Industrial partner):
 Co PI, leading the business activity
Prof. Dr. Omaima Elgazayerly leading pharm. activity
Prof. Dr. Ali Abdel-Aziz  consultant for food sc.
activity

16. Expected Project Outcomes and Impact
1. Expected project out puts (products and prototype)
- Pilot scale unit for pectin production from orange peels.
- Dried, ground pectin peels ready for pectin extraction or
for export at high price (lateral output).
- Dried pectin produced on industrial scale
- Pectin based Pharmaceutical dosage formula
2. Publications
- Expected patent concerning pectin preparation
- Publications about orange oil, bioactives, pectin
preparation and extraction on industrial scale.
- M.Sc. Thesis about raw material preparation from food
stream residues.

17. Expected Project Outcomes and Impact (cont..)
3. Business development
- Up scaling of the project through large Egyptian
enterprise in the field of orange business.
- Registration at MOH for Pectin based products to be sold
as Nutraceutical and/or functional food
- Local marketing of the produced pectin.

18. It All Starts with
RICE BRAN INNOVATIVE STABILIZATION

19. Previous Success Story
Rice Residue managment
What is Rice Bran?
•Rice Bran is a byproduct of the milling process (conversion
of brown rice to white rice
•It is reported to have 65% of the nutritive value of the
whole rice grain
•In Egypt it is discarded or used as animal feed due to its
extreme instability that renders it rancid and thus unfit for
human consumption
What is a Nutraceutical?
A product isolated or purified from foods, and generally sold
in medicinal forms and demonstrated to have a physiological
benefit or provide protection against chronic disease

20. Different Implemented
Projects (2008-2012)
A)
Rice Bran Stabilization (lab Scale) & (Industrial
Scale)
B)
Phytochemical and Nutritional study of the Stabilized
Rice bran.
C)
Implementations of the different rice bran products
in food , pharmaceutical and cosmetic industries .

21. Rice Bran – Stabilization Unit 1
Capable of stabilizing the bran of 150 MT rice milling / day

22. Rice Bran – Stabilization Unit 2
Capable of stabilizing the bran of 150 MT rice milling / day

23. Rice Bran Nutraceuticals
 Contract Number
C2/S1/118
 Total cost of the action
EURO 192000
 Project Duration
18 months
 Project PI
Prof. Dr. M.T. Khayyal
 Project Coordinator
Dr. Amr M. Helal

24. Products
• Stabilized Rice Bran soft Gel Capsules
(Under registration; Riciplex)
• Rice Bran sachets (10gm; ORYZA, Under
registration).
• School Nutrition Program ( in cooperation
with Ain Shams University)
• Functional Food (flakes , in cooperation with
Mass Food Company)

25. Publications
•
Helal AM, Khayyal MT, Abd El Aziz HM, Abdel Salam RM
Developing a nutraceutical from Egyptian Stabilized rice bran: pharmacological approach
PlantaMed 2011:77: 1229-1472 Georg Thieme Verlag KG Stutgart. New Yourk. ISSN 0032-094
•
Ramadan, Shimaa El-Saed, Amr M. Helal, Comparative Study on Egyptian Rice Bran Extracted
by Solvents and Supercritical CO2. Advances in Food Sciences, 35 (1), 2013, 23-29.
(Germany). ( Annex 2)
•
Sahar Y. Al-Okbi, Nagwa M. Ammar, Doha A. Mohamed, Ibrahim M. Hamed, Ahmad, H.
Desoky, Hagar F. El-Bakry, and Amr M. Helal. Egyptian rice bran oil: chemical analysis of the
main phytochemicals. Accepted in La Rivista Italiana delle Sostanze Grasse in 25 January,
2013 (Italy).
•
Sahar Y. Al-Okbi, Ahmed M.S. Hussein, Ibrahim M. Hamed, - Doha A. Mohamed and Amr M.
Helal, Chemical, Rheological, Sensorial and Functional Properties of Gelatinized Corn- Rice
Bran Flour Composite Corn Flakes and Tortilla Chips, Journal of Food Processing and
Preservation, 2012 (Wiley) , doi:10.1111/j.1745-4549.2012.00747.x ( annex 4)

26. Publication (Cont.)
•
Hussein O Ammar, Sahar Y Al-Okbi, Dina M Mostafa and Amr M. Helal, Rice bran oil: Preparation
and evaluation of novel liquisolid and semisolid formulations. International Journal of
Pharmaceutical compounding, 16 (6), 2012, 516-523 (USA).
•
Rice bran solid lipid nanoparticles: Preparation and characterization Mohamed Hassan Hany and
Omaima Naim El Gazayerly* Department of Pharmaceutics and Industrial Pharmacy, Faculty of
Pharmacy, Cairo University, Cairo, Egypt. Available online at http://www.urpjournals.com
International Journal of Research in Drug Delivery Universal Research Publications
•
Kaup RM, Khayyal MT, Verspohl EJ.Department of Pharmacology, Institute of Medicinal Chemistry,
University of Muenster, Hittorfstr. 58-62, 48149, Münster, Germany, Antidiabetic effects of a
standardized Egyptian rice bran extract.
Phytother Res. 2013 Feb;27(2):264-71. doi: 10.1002/ptr.4705. Epub 2012 May 8
•
Stephanie Hagl; Alexa Kocher; Christina Schiborr; Schamim Eckert; Ion Ciobanu; Marc Birringer,
Ph.D.; Hesham Al-Askary, Ph.D.; Amr Helal, Ph.D.; Mohamed T Khayyal, Ph.D.; Jan Frank, Ph.D.;
Walter E Muller, Ph.D.; Gunter P. Eckert, Ph.D. Rice bran extract protects from mitochondrial
dysfunction in guinea pig brains.

27. Success Story in the Media
www.ricencare.com

29. Awards

30. Awards (Cont.)