MSD Finca Productiva Reproduccion

1. São Paulo - SP 07/04/2009 Carlos Antônio de Carvalho Fernandes Med. Vet. PhD. [email_address]

4. Uterine infections in cattle

5. Uterine infections in cattle Volume of uterine cavity in cattle 1 days post calving=6 liters-9kg 30 days post calving=0.2 liters – 0.5kg

15. Treatment PUERPERAL INFECTION Excessive Contamination Delayed involution Antibiotics Prostaglandins

17. Efficacy of the treatment of placental retention with oxytetracycline HCl with or without simultaneous administration of cloprostenol (Fernandes, 1999) * Significativo pelo teste de ‘t’ a 5% de probabilidade Variable Oxytetracycline HCl Oxyt+ Cloprostenol Number of animals 90 90 Infection at 30d post partum (%) 44.4 42.2 Degree of uterine involution* (%) 1.92 2.43 Interval calving-to-conception (d)* 78.4 54.1 Breeding period (d)* 131.3 111.7 Services/conception 2.41 2.03

19. Carlos Antônio de Carvalho Fernandes Eduardo Ramos de Oliveira Bruno Fernandes Ludgero Alves Ana Cristina Silva de Figueiredo Marilu Martins Gioso

20. Treatment scheme in animals after detection of calving problems Group Treatment N At 20 h pp 48 h post 1st application 1 Nuflor IM2x 26 20mg de Florfenicol IM/kg PV 20mg de Florfenicol IM/kg PV 2 Nuflor IM2x +Ciosin 28 20mg de Florfenicol IM/kg PV + 0,530mg de Cloprostenol 20mg de Florfenicol IM/kg PV + 0,530mg de Cloprostenol 3 Nuflor SC1x 27 40mg de Florfenicol SC/kg PV - 4 Nuflor SC1x +Ciosin 27 40mg de Florfenicol SC/kg PV + 0,530mg de Cloprostenol 0,530mg de Cloprostenol 5 Oxitetra IM1x 29 20mg de Oxitetraciclina IM/kg PV 6 Oxitetra IM1x + Ciosin 28 20mg de Oxitetraciclina IM/kg PV + 0,530mg de Cloprostenol 0,530mg de Cloprostenol TOTAL 165

22. Occurrence and average percentage of uterine infections and characteristics of the infection in different groups of animals evaluated between 25 and 35 days pp (Fernandes et al., 2008) P> 0,05 Group Treatment Type N N and % of animals with uterine infection Grade 1 No and % Grade 2 No and % Grade 3 No and % 1 Nuflor IM2x 26 11 - 42,31 5 – 19,23 3 – 11,54 3 – 11,54 2 Nuflor IM2x +Ciosin 28 9 - 32,14 4 – 14,29 4 – 14,29 1 – 3,57 3 Nuflor SC1x 27 10 - 37,03 5 – 18,52 3 – 11,11 2 – 7,41 4 Nuflor SC1x +Ciosin 27 8 - 29,63 4 – 14,81 4 – 14,81 0 – 0,00 5 Oxitetra IM1x 29 11 - 37,93 4 – 13,79 4 – 13,79 3 – 10,34 6 Oxitetra IM1x + Ciosin 28 9 - 32,14 4 – 14,29 3 – 10,71 2 – 7,14 TOTAL 165 35,15 15,76 12,73 6,66

23. Days post partum at first service, number of services per conception and breeding period in animals in different treatment groups (Fernandes et al., 2008) P> 0,05 Group Treatment type No Calving-to-1 st service interval (days) Services/ conception Breeding period (days) 1 Nuflor IM2x 26 75,32 + 19,87 3,27 + 1,11 169,29 + 45,76 2 Nuflor IM2x +Ciosin 28 62,45 + 19,62 2,91 + 0,99 148,63 + 39,97 3 Nuflor SC1x 27 66,27 + 16,66 2,96 + 0,86 157,33 + 41,18 4 Nuflor SC1x +Ciosin 27 56,19 + 14,09 2,48 + 0,83 135,49 + 35,64 5 Oxitetra IM1x 29 68,17 + 15,98 3,11 + 0,89 161,02 + 43,53 6 Oxitetra IM1x + Ciosin 28 60,83 + 16,37 2,92 + 0,93 149,24 + 44,60 TOTAL 165 65,08 + 17,47 2,96 + 0,98 154,56 + 42,01

26. Sequence of events associated with uterine infections

29. Post-puerperal uterine infections Endometritis

30. Post-puerperal uterine infections Endometritis

31. Mucosal discharge

32. Comparative efficacy of tow methods for diagnosis of uterine infections in cattle: rectal palpation and vaginoscopy Method Number of animals Efficacy (%) Vaginoscopy 738 100 Rectal palpation 260 35,2

37. Bruno Fernandes Ludgero Alves 1 ; Eduardo Ramos e Oliveira 1 , Carlos Antônio de Carvalho Fernandes 2 ; Marilu Martins Gioso 2

38. Efficacy of cloprostenol (Ciosin®) in the treatment of uterine infections in cattle Treatment scheme and number of animals in accordance with the classification of the uterine infection and presence of ovarian activity (CL) Group Ovarian Grade of Uterine Infection (treatment) Activity (class) N Grade 1 Grade 2 Grade 3 Controle CL (+) 15 5 5 5 CL (-) 15 5 5 5 1 dose CL (+) 32 15 10 7 CL (-) 32 15 10 7 2 doses-24Horas Com CL 32 15 10 7 CL (-) 32 15 10 7 2 doses-48Horas CL (+) 32 15 10 7 CL (-) 32 15 10 7 Total 222 100 70 52

39. Average and percentage occurrence of different types of uterine infections in animals with or without corpus luteum in accordance with the characteristics of uterine discharge detected with vaginoscopy (Alves et al., 2008)

40. Average and percentage occurrence of different types of uterine infections in animals with or without corpus luteum in accordance with the characteristics of uterine discharge detected with vaginoscopy (Alves et al., 2008)

41. Eficiência m é dia geral (Número e Percentual) dos tratamentos de infec ç ão uterina em animais com presen ç a ou não de Corpo L ú teo (Alves et al 2008). CL (+) CL (-) Group Development of the condition Improvement (treatment) N Improvement Cure and cure Control 15 2 (13,33%) a 2 (13,33%) a 4 (26,67%) a 1 dose 32 5 (15,62%) a 10 (31,25%) b 15 (46,88%) b 2 doses-24Horas 32 5 (18,75%) a 20 (65,62%) c 25 (71,88%) c 2 doses-48Horas 32 7 (21,85%) a 15 (46,87%) b 22 (68,75%) c Total 111 19 (17,12%) 47 (42,34%) 66 (59,46%) Group Development of the condition Improvement (treatment) N Improvement Cure and cure Control 15 2 (13,33%) a 1 (6,67%) a 3 (23,33%) a 1 dose 32 5 (15,63%) a 10 (31,25%) b 15 (46,87%) b 2 doses-24Horas 32 5 (15,63%) a 16 (50,00%) b 21 (65,63%) b 2 doses-48Horas 32 8 (25,00%) a 12 (37,50%) b 20 (62,50%) b Total 111 20 (18,02%) 39 (35,14%) 60 (50,05%)

42. Average percentage of positive development (improvement and cure) of the uterine infections in animals with or without CL, independently from the type of treatment (Alves et al., 2008)

43. Carlos Antônio de Carvalho Fernandes 2 ; Marilu Martins Gioso 2 ;Bruno Fernandes Ludgero Alves 1 ; Eduardo Ramos e Oliveira 1

44. Average efficacy (numeric and percentage) of the treatments independently from the presence of CL Group Development of the condition (treatment) N Improvement Cure Improvement and Cure 1: Control 30 3 a 3 a 6 ( 20,00% ) a 2: Preloban 64 9 b 17 b 26 ( 40,62% ) b 3: Metricure 64 11 b 30 c 41 ( 64,06% ) c 4: Preloban + Metricure 64 14 b 36 c 50 ( 78,12% ) c Total 222 37 86 123

45. Average efficacy of evaluated treatment approaches in cows with or without the presence of CL

46. Average interval treatment-to-conception in different treatment groups in cows with or without the presence of CL

47. “ O sucesso nasce do querer. Sempre que o homem aplicar a determinação e a persistência para um objetivo, ele vencerá os obstáculos, e, se não atingir o alvo, pelo menos fará coisas admiráveis”. José de Alencar Carlos Antônio de Carvalho Fernandes Med. Vet. D.Sc. [email_address]