22301 Analytical Techniques In Bio Technology Set1

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Code No: RAR05322301
RA
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
ANALYTICAL TECHNIQUES IN BIO TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks

1. Explain the following terms associated with Fluorescent Microscope.

(a) Barrier lter
(b) Heat lter

(c) Fluorochromes
(d) Exciter lter. [4+4+4+4]

2. What is the di erence between a TEM and a SEM? [16]

3. How is blo od sugar measured? [16]

4. What are the factors a ecting electrophoretic mobility of any molecule? [16]

5. Explain the basic principle behind all forms of Chromatography [16]

6. How is PCR used to sequence DNA? [16]

7. Write short notes on

(a) Rocket Immunoelectroforesis
(b) Radial Immunoassays.
[8+8]

8. Explain

(a) Substrate uptake in absence of product formation.
(b) Substrate uptake with product formation. [8+8]

1 of 1

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22301 Analytical Techniques In Bio Technology Set1

1. www.studentyogi.com www.studentyogi.com Code No: RAR05322301 RA III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 ANALYTICAL TECHNIQUES IN BIO TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. Explain the following terms associated with Fluorescent Microscope. (a) Barrier lter (b) Heat lter (c) Fluorochromes (d) Exciter lter. [4+4+4+4] 2. What is the di erence between a TEM and a SEM? [16] 3. How is blo od sugar measured? [16] 4. What are the factors a ecting electrophoretic mobility of any molecule? [16] 5. Explain the basic principle behind all forms of Chromatography [16] 6. How is PCR used to sequence DNA? [16] 7. Write short notes on (a) Rocket Immunoelectroforesis (b) Radial Immunoassays. [8+8] 8. Explain (a) Substrate uptake in absence of product formation. (b) Substrate uptake with product formation. [8+8] 1 of 1 www.studentyogi.com www.studentyogi.com