Most potential drug candidates (90%) fail within the clinical trials, mainly because of lack of efficacy. What if the pharmaceutical industry uses predictive human in vitro models in early drug discovery ?

1. Introduction
What is the extracellular matrix (ECM)?
Conclusion and perspectives
Nathalie Maubon1*, Marie Lesaffre1, Véronique De Conto1, Elodie Vandenhaute1, Zied Souguir1
A groundbreaking 3D cell culture technology for HCS:
BIOMIMESYS hydroscaffold
1 HCS pharma, 250 rue Salvador Allende, Biocentre Fleming Bâtiment A, 59120 Loos, FRANCE
Results
What is hydroscaffold?
To properly mimic human tissues, the ECM should be included in in
vitro models. BIOMIMESY properly reproduces the ECM, and is
available:
• glycosaminoglycans (essentially Hyaluronic Acid, HA)
• structural (mainly collagens)
• adhesion proteins (laminins, fibronectin)
 ECM properties are crucial in
maintaining organ’s homeostasis!
BIOMIMESYS® = HA grafted with other ECM
components (collagens, adhesion peptides…),
which composition and physico-chemical properties
are tailored to mimic the ECM of each organ/tissue.
References
We are working hard to develop new BIOMIMESYS® products:
Liver
Decellularized human
adipose tissue
IN VIVO Hyaluronic
sheets
Collagen
fibers
IN VITRO
- Primary human white adipocytes in
Adipored™
 Promoted accumulation
of triglycerides along time
The ECM has diverse and crucial roles, in
health and disease:
ECM =
ECM composition & physicochemical properties depend on the
organ/tissue :
Most potential drug candidates
(90%) fail within the clinical trials,
mainly because of lack of efficacy.
Source: AstraZeneca
What if the pharmaceutical
industry uses predictive
human in vitro models in
early drug discovery ?
IN VITRO
Hyaluronic
sheets
Collagen
fibers
IN VIVO
Decellularized human
liver
 BIOMIMESYS® structure is the same as the in vivo ECM (decellularized human tissue)
 BIOMIMESYS® helps the long-term maintenance of cellular differentiation, compared to 2D
- Primary human hepatocytes in
 Maintained differentiation of hepatocytes
- Human neuronal cells in
 Formation of ‘mini-brains’
 BIOMIMESYS® promotes the maintenance of cellular functionality
- Lipogenesis model in - Cholestasis model in
RA, retinoic acid
 Higher lipogenesis in BIOMIMESYS, and 30% lipogenesis
inhibition by RA
 Induction of cholestasis by CPZ
[chlorpromazine (CPZ)]
- Immune & cancer cells in
Luhmes cells
Comparison of BIOMIMESYS® to other 3D systems
 BIOMIMESYS® allows coculture and
live cell imaging
Human cancer
cell line (HCT116)
+ primary human
macrophages
NEW
384-well plates: Liver pathology:
Louis et al., 2017. A biomimetic hydrogel functionalized with
adipose ECM components as a microenvironment for the 3D
culture of human and murine adipocytes. Biotechnol Bioeng,
114(8):1813-1824.
For more information, go to : biomimesys.com or
hcs-pharma.com or scan this QR-code
1* : nathalie.maubon@hcs-pharma.com