BIOMIMESYS® range are hyaluronan based scaffolds developed to overcome the 2D flat culture limitations by recreating an in vivo physiology within the in vitro environment. BIOMIMESYS® Adipocyte scaffold is made of RGDS-grafted Hyaluronic acid (1.6 MDa), Adipic acid dihydrazide crosslinker and extracellular matrix (ECM) proteins (collagen type I and collagen type VI) to mimic fat tissue-ECM composition.

1. BIOMIMESYS®Adipose tissue, a relevant in vitro adipocyte 3D model
BIOMIMESYS®Adipose tissue has been tested on 3T3-L1, 3T3-F44 cells and on human cryopreserved preadipocytes (HWP). It makes 3D cell culture
easy and provides a robust in vitro and reliable model for metabolism studies such as obesity or diabetes and drug discovery.
To know more about BIOMIMESYS®Adipose tissue visit our website: www.biomimesys.com
 +33(0) 769 999 137; hello@biomimesys.com
BIOMIMESYS® Adipose tissue is ready to use
Available in a ready-to-use format (96 well plates) it enables the culture of adipocytes under
physiological conditions that are representative of the microenvironment found in adipose tissue(2).
Adipocytes cells are simply seeded on top of the hydroscaffold and placed in the incubator.
The media can be refreshed easily by pipetting.
BIOMIMESYS® Adipose tissue is physiological
BIOMIMESYS® range are hyaluronan based hydroscaffolds developed to
overcome the 2D flat culture limitations by recreating an in vivo
physiology within the in vitro environment.
BIOMIMESYS®Adipose tissue hydroscaffold is made of RGDS-grafted
Hyaluronic acid (1.6 MDa), Adipic acid dihydrazide crosslinker and
extracellular matrix (ECM) proteins (collagen type I and collagen type VI)
to mimic fat tissue-ECM composition.
In BIOMIMESYS®Adipose tissue
PHYSICOCHEMICAL FEATURES
Porosity: 70-170 µm
Young’s modulus: E = 0.45 ± 0.05kPa
Swelling ratio = 60 ± 10g/g
Adipocyte
maturation
BIOMIMESYS®Adipose tissue is compatible with all analytical technologies
Being transparent makes it suitable for microscopy (immunofluorescence, brightfield) and use in plate readers (OD, fluorescence).
Thanks to its porosity, proteins and nucleic acid can be extracted by directly adding the lysis buffer to the hydrogel.
In comparison to in vivo
decellularized adipose tissue
SEM observation of a human decellularized
adipose tissue (from Wang et al., 2013 (1) )
Perilipin immunostaining – HWP
a maturation marker : the lipid droplet-associated protein
Morphology
SEM observation of a BIOMIMESYS®Adipose tissue section,
highlighting the collagen chain, (artificially coloured)
Biomimetic structure
In vivo
References:
(1) L. Wang et al. Combining decellularized human adipose tissue extracellular matrix and adipose-derived stem cells for adipose tissue engineering, Acta Biomaterialia 2013 (9):8921-8931.
(2) G. J. Hausman. Meat Science and Muscle Biology Symposium: The influence of extracellular matrix on intramuscular and extramuscular adipogenesis, Journal of Animal Science 2012 (90):942-949.
(3) M. Kurita et al. Influences of Centrifugation on Cells and Tissues in Liposuction Aspirates: Optimized Centrifugation for Lipotransfer and Cell Isolation, Plastic and Reconstructive Surgery 2008 121(3):1033-1041.
50 µm
200 µm
Murine Preadipocytes 3T3-
L1 at day 7
Human White Preadipocytes (HWP)
at day 14
10µm 20µm
Human adipocytes clusters from
abdomen liposuction
(from Kurita et al., 2008 (3) )
In vivo-like organisation of adipose tissue in
BIOMIMESYS®Adipose tissue.
N=3, two-ways ANOVA statistics with Tukey post-hoc ; * : p<0,05 ; ** : p<0,01 ; *** : p<0,001
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Better lipogenic activity and higher differentiation rate in
BIOMIMESYS®Adipose tissue compared to 2D culture.
LSCM observation of Z-stack at day 21.
Chronic effect on lipogenesis – 3T3-L1
DIFFERENTIATION
48h 72h
NUTRITION
D0
GROWTH
D14D7 D28
± Retinoic acid (RA)
N=3, two-ways ANOVA statistics with Tukey post-hoc ; * : p<0,05 ; ** : p<0,01 ; *** : p<0,001
Drug screening
HWP undergo complete maturation
using BIOMIMESYS®Adipose tissue.
100µm
Perilipin
Nucleus
Lipids accumulation – 3T3-L1
AdipoRed™ Assay Reagent
Adipogenesis - HWP
mRNA of a early gene : fatty acid binding protein 4 (FABP4)
Adipogenesis - HWP
mRNA of a late gene :
glucose transporter type 4 (GLUT-4)
Acute effect on lipolysis – 3T3-F442A
DIFFERENTIATION
48h 72h
NUTRITION
D0
GROWTH
D12
± Isoprenalin or Caffein
0
0,5
1
1,5
2
2,5
day 12
Glycerol[µM]
Glycerol release in
BIOMIMESYS®Adipose tissue
0 µM 1 µM 10 µM
0
0,5
1
1,5
2
2,5
day 12
Glycerol[µM]
Glycerol release in
BIOMIMESYS®Adipose tissue
0 mM 5 mM 10 mM
Preadipocytes and adipocytes cultured in BIOMIMESYS®Adipose tissue can
be used as models for lipogenesis and lipolysis analysis.
90mins
Isoprenalin treatment Caffein treatment
+50%
+60%
Adipocyte differentiation
0
10
20
30
40
50
60
day7 day14 day28
RFUAdipoRed/DNA
Lipids accumulation in BIOMIMESYS® Adipose tissue
without RA 1µM RA 5µM RA 10µM RA
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BIOMIMESYS®Adipose tissue
BIOMIMESYS®Adipose tissue
0
1
2
3
4
5
6
7
8
day1 day7 day14 day21 day28
FABP4/RPII
FABP4 mRNA expression
2D BIOMIMESYS®Adipocytes
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BIOMIMESYS®Adipose tissue
0
5
10
15
20
25
30
35
40
45
50
day1 day7 day14 day21 day28
RFUAdipoRed/DNA
3T3-L1
2D BIOMIMESYS®AdipocyteBIOMIMESYS®Adipose tissue
0
0,2
0,4
0,6
0,8
1
1,2
day7 day14 day21 day28
GLUT-4/RPII
GLUT-4 mRNA expression
2D BIOMIMESYS®AdipocyteBIOMIMESYS®Adipose tissue