Microsatellites for diseases resistant

Ibnu Sahidhir
Healthy

Sick
DNA

Microsatellites

CACACACACACACA

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www.artaquaculture.blogspot.com

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Overview to Aquaculture

Better genetic
Better genetic Better
Better
make up
make up Survival Rate
Survival Rate

Seeds

Company Water Marketable size Harvest Profit

Feeds

+
= “Culturing aquatic organisms to get profit”
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Early Mortality Syndrome (Lightner, 2012)

Left shrimp ponds

High mortality

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Don’t argue !
Test your DNA

No, he’s
my lost baby !

He’s really
my lovely son !

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This short
DNA make me
sick !

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The topic

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“Microsatellites are repetitions of simple DNA sequences”

Primer target Flanking region
Flanking region Microsatellite
Microsatellite
(bold part) (red part)
(red part) (AG…)
(AG…)

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Type I : Coding microsatellites (inside genes)

Type II : Non-coding microsatellites

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DNA Extraction
12,000 g 10
minutes

Tissue Extracted Cell
Add
by extraction
sample buffer
Chloroform

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Microsatellite Isolation: 1st Hybridization

Microsatellit
Microsatellit
Microsatel
Microsatel e e
lite
lite

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Microsatellite Isolation:
2nd Hybridization and Designing New Primer
DNA sample

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Evaluate in
population

Multiply them
Extract in PCR
microsatellites

Find their pattern using DNA sequencer

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DNA size (bp) 300 70
1st generation
Healthy 100% 10%

Sick 100% 90%

2nd generation
Healthy 100% 5%

Sick 100% 95%
etc.

“This ‘70’ must be the problem !”

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1. Search White Spot Syndrome Virus/WSSV-Resistant
Black Tiger Shrimp
(Mukherjee and Mandal, 2009)

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2. Search Chinese Shrimp Resistant to
White Spot Syndrome Virus/WSSV
(Dong et al, 2008)

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(No samples)

Double band belongs to healthy shrimp

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Flounder attacked
by limphocystis virus

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B A
= sick family = healthy family

AB
=Cross AxB

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Father Mother
=Steelhead trout =Rainbow trout

Children

IHNV
Challenge

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 High mortality could be prevented by select diseases resistant
brood stocks.
 Evidences show that microsatellites can detect susceptible
and resistant strain to specific diseases
 Conclusively, microsatellites is a powerful marker in selective
breeding

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Microsatellites for diseases resistant

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Microsatellites for diseases resistant

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