1. A role for Pab1p in PUF-mediated translational repression
JJ Chritton
Wickens Lab
University of Wisconsin Madison

2. PUF proteins bind RNA
Human Pum1 Wang et al. 2002

3. PUF proteins regulate mRNA expression

4. Puf5 represses targeted mRNAs
?

5. Poly(A) binding protein in translation

6. Cell-free translation assay

7. Puf5p can repress a reporter in vitro

8. Two mutations disrupt Puf5 repression

9. Oligo(A) tracts enhance Puf5 repression

10. PAB1 is required to repress HO

11. PAB1 is required for Puf5 to repress other targets

12. PAB1 mediates repression through oligo(A) tracts

13. PAB1 binds the HO 3’UTR

14. PAB1 domains

15. PAB1 competition assay

16. The C-terminus of PAB1 is required for repression

17. The MLLE domain of PAB1 is required for repression

18. Puf5 may inhibit a function of PAB1

19. … or PAB1 may participate in repression

20. Acknowledgements
Marv Wickens Allan Jacobson
Shuby Ghosh
Wickens lab Funding
Amy Cooke NIH training grant
Cary Valley NIH grant to MW
Chris Lapointe
Daniel Wilinski
Jacque Baca
Yvonne Koh
Zak Campbell