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CHAPTER 3
MATERIALS AND METHODS
Subsections/Subtopics
• Research Method/Design
• Materials
• Equipment/Apparatus and
Utensils
• Procedure
• Statistical Treatment
RESEARCH METHOD
EXPERIMENTAL DESIGN
-It refers to the complete sequence of steps
undertaken to answer the research problem.
-It is a problem-solving approach that the
study is described in the future on WHAT
WILL BE when a variables are carefully
controlled or manipulated.
BASIC PRINCIPLES
1. REPLICATION – repetition of the basic
experiment.
2. RANDOMIZATION – assignment of the
experimental units to the treatment or vice
versa by chance.
3. LOCAL CONTROL – balancing,
grouping and blocking of the experimental
units that are employed in the adopted
design.
TYPES OF EXPERIMENTAL
DESIGN
1. SINGLE-GROUP DESIGN – single treatment
with two or more levels.
EFFECT OF FISH MEALAS SUPPLEMENTAL FEED
UPON THE YIELD OF MUDCRAB CULTURED IN THE
FISHPOND
Fish Meal Treatment (T)
Yield of Mudcrab (kg)
Replications
T1 (5%)
T2 (10%)
T3 (15%)
1 2 3
x x x
x x x
x x x
2. TWO-GROUP DESIGN – two comparable
groups are employed as experimental and control
groups or two comparable groups are both
experimental groups.
GROWTH RATE OF CULTIVATING EUCHEUMA USING
BROADCASTING AND LANTAY METHODS
Broadcasting Method
(control Group)
Lantay Method
(Experimental)
x
x
x
x
x
x
x
x
x
x
3. TWO-PAIR GROUP DESIGN - an elaboration
of the two-group design wherein there are two
control groups and two experimental groups.
Ex. ACCEPTABILITY OF CANNED MILKFISH BONE MEAL IN
SALMON AND FRENCH STYLES WITH AND WITHOUT SEA
VEGETABLE.
PANELISTS
Canned Milkfish Bone Meal
Control Group
(Without Sea Vegetable)
Experimental Group
(With Sea Vegetable)
1 x x x x
2 x x x x
3 x x x x
4 x x x x
4. PARALLEL-GROUP DESIGN – two or more
groups are used as the same time with only a single
variable (control) manipulated or changed.
GROWTH RATE OF CULTIVATING EUCHEUMA USING
BROADCASTING AND LANTAY METHODS
Broadcasting Method (kg)
(control Group)
Lantay Method (kg)
(Experimental)
x
x
x
x
x
x
x
x
x
x
5. COUNTERBALANCED OR LATIN SQUARE
DESIGN – exchange of two or more treatments
taken by the subjects during the experiment.
Ex. CULTURING OF GROUPER USING FOUR
SUPPLEMENTAL FEEDS TO FOUR FISH CAGES
FC1 FC2 FC3 FC4
SF1
SF2
SF3
SF4
G1 G2 G3 G4
G2 G3 G4 G3
G3 G4 G1 G2
G4 G1 G2 G1
6. COMPLETE RAMDOMIZED DESIGN
(CRD) – a group of test plants or animals is
studied only once but subsequent treatment is
applied to determine the cause of change.
Ex. YIELD OF MUDCRAB USING BREAD MEAL AS
SUPPLEMENTAL FEED
% Supplemental Feed per Compartment
Sampling
1 2 3
1 (3%)
2 (5%)
3 (7%)
4 (10%)
5 (15%)
x x x
x x x
x x x
x x x
x x x
7. RANDOMIZED COMPLETE BLOCK DESIGN
(RCBD) – group of test plants and animals as subjects of
the study which are studied only once but subsequent
treatment is applied are replicated to determine the cause of
change.
Ex. CULTURING ABALONE IN FISH CAGES USING FISH MEAL, SHRIMP
MEAL AND FISH SILAGE AS SUPPLEMENTAL FEEDS
Supplemental Feed
Replications
1 2 3
Fish Meal
Shrimp Meal
Fish Silage
x x x
x x x
x x x
8. CORRELATIONAL DESIGN –
determine the relationship of the two
dependent variables on how they are
manipulated by the independent variables.
Ex. Culturing Milkfish on the Fishpond Using Fish
Silage as Supplemental Feed
Milkfish
Weight (X)
grams
Length (Y)
cm
1
2
3
4
x
x
x
x
x
x
x
x
EXAMPLE:
The materials include the ingredients and its
formulations. The ingredients must be
quantified.
MATERIALS
EXAMPLE:
States the equipment and utensils used
in the preparation of the study.
EQUIPMENT AND
UTENSILS
EXAMPLE:
PROCEDURE
It explains comprehensively
the process in preparing the
product.
EXAMPLE:
STATISTICAL TREATMENT
Diagnose the most appropriate statistical
tools used for experimental design to answer
the specific questions and accept/reject the
hypotheses presented in chapter 1.
THANK YOU.
REFERENCE:
Calmorin, Melchor & Laurentina. 2012;
Research Methods and Thesis Writing;
2nd Edition

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Chapter 3 Research with Examples

  • 2. Subsections/Subtopics • Research Method/Design • Materials • Equipment/Apparatus and Utensils • Procedure • Statistical Treatment
  • 4. EXPERIMENTAL DESIGN -It refers to the complete sequence of steps undertaken to answer the research problem. -It is a problem-solving approach that the study is described in the future on WHAT WILL BE when a variables are carefully controlled or manipulated.
  • 5. BASIC PRINCIPLES 1. REPLICATION – repetition of the basic experiment. 2. RANDOMIZATION – assignment of the experimental units to the treatment or vice versa by chance. 3. LOCAL CONTROL – balancing, grouping and blocking of the experimental units that are employed in the adopted design.
  • 6. TYPES OF EXPERIMENTAL DESIGN 1. SINGLE-GROUP DESIGN – single treatment with two or more levels. EFFECT OF FISH MEALAS SUPPLEMENTAL FEED UPON THE YIELD OF MUDCRAB CULTURED IN THE FISHPOND Fish Meal Treatment (T) Yield of Mudcrab (kg) Replications T1 (5%) T2 (10%) T3 (15%) 1 2 3 x x x x x x x x x
  • 7. 2. TWO-GROUP DESIGN – two comparable groups are employed as experimental and control groups or two comparable groups are both experimental groups. GROWTH RATE OF CULTIVATING EUCHEUMA USING BROADCASTING AND LANTAY METHODS Broadcasting Method (control Group) Lantay Method (Experimental) x x x x x x x x x x
  • 8. 3. TWO-PAIR GROUP DESIGN - an elaboration of the two-group design wherein there are two control groups and two experimental groups. Ex. ACCEPTABILITY OF CANNED MILKFISH BONE MEAL IN SALMON AND FRENCH STYLES WITH AND WITHOUT SEA VEGETABLE. PANELISTS Canned Milkfish Bone Meal Control Group (Without Sea Vegetable) Experimental Group (With Sea Vegetable) 1 x x x x 2 x x x x 3 x x x x 4 x x x x
  • 9. 4. PARALLEL-GROUP DESIGN – two or more groups are used as the same time with only a single variable (control) manipulated or changed. GROWTH RATE OF CULTIVATING EUCHEUMA USING BROADCASTING AND LANTAY METHODS Broadcasting Method (kg) (control Group) Lantay Method (kg) (Experimental) x x x x x x x x x x
  • 10. 5. COUNTERBALANCED OR LATIN SQUARE DESIGN – exchange of two or more treatments taken by the subjects during the experiment. Ex. CULTURING OF GROUPER USING FOUR SUPPLEMENTAL FEEDS TO FOUR FISH CAGES FC1 FC2 FC3 FC4 SF1 SF2 SF3 SF4 G1 G2 G3 G4 G2 G3 G4 G3 G3 G4 G1 G2 G4 G1 G2 G1
  • 11. 6. COMPLETE RAMDOMIZED DESIGN (CRD) – a group of test plants or animals is studied only once but subsequent treatment is applied to determine the cause of change. Ex. YIELD OF MUDCRAB USING BREAD MEAL AS SUPPLEMENTAL FEED % Supplemental Feed per Compartment Sampling 1 2 3 1 (3%) 2 (5%) 3 (7%) 4 (10%) 5 (15%) x x x x x x x x x x x x x x x
  • 12. 7. RANDOMIZED COMPLETE BLOCK DESIGN (RCBD) – group of test plants and animals as subjects of the study which are studied only once but subsequent treatment is applied are replicated to determine the cause of change. Ex. CULTURING ABALONE IN FISH CAGES USING FISH MEAL, SHRIMP MEAL AND FISH SILAGE AS SUPPLEMENTAL FEEDS Supplemental Feed Replications 1 2 3 Fish Meal Shrimp Meal Fish Silage x x x x x x x x x
  • 13. 8. CORRELATIONAL DESIGN – determine the relationship of the two dependent variables on how they are manipulated by the independent variables. Ex. Culturing Milkfish on the Fishpond Using Fish Silage as Supplemental Feed Milkfish Weight (X) grams Length (Y) cm 1 2 3 4 x x x x x x x x
  • 15. The materials include the ingredients and its formulations. The ingredients must be quantified. MATERIALS
  • 17. States the equipment and utensils used in the preparation of the study. EQUIPMENT AND UTENSILS
  • 19. PROCEDURE It explains comprehensively the process in preparing the product.
  • 21. STATISTICAL TREATMENT Diagnose the most appropriate statistical tools used for experimental design to answer the specific questions and accept/reject the hypotheses presented in chapter 1.
  • 22. THANK YOU. REFERENCE: Calmorin, Melchor & Laurentina. 2012; Research Methods and Thesis Writing; 2nd Edition