A detailed description about the basic steps involved in the - PCR - Polymerase Chain Reaction, its applications,its limitations and steps to overcome it.

1. Polymerase Chain Reaction (PCR)

7. DNA Molecule Adenine Thymine Guanine Cytosine

16. PCR Cycles

17. PCR Cycles

18. Denaturing Template Heat causes DNA strands to separate Denature DNA strands 94 o C 3’ 5’ 5’ 3’ 5’ 3’ 3’ 5’

19. PCR Cycles

21. PCR Cycles

23. PCR Cycles

24. The target product is made in the third cycle 3 ’ 5’ 3 ’ 5’ 3’ Cycle 1 Cycle 2 Cycle 3 3’ 5’ 5’ 3’ 5’ 5’ 3’ 5’ 3’ 5’ 3’ 3’ 3’ 3’ 3’ 5’ 5’ 5’ 5’

37. Shanthilal.J III B.Tech., Biotechnology, K.S.Rangasamy College of Technology Tiruchengode. By,