1. 1
Research Title
Prevalence and antibiotic sensitivity of
enterohaemorrhagic Escherichia coli O157: H7 in cattle on
smallholdings in Dhaka, Bangladesh
Submitted to
Government of the People’s Republic of Bangladesh
Ministry of Science and Technology
Dhaka, Bangladesh
Submitted by
Asst. Prof. Dr. Md. Kamrul Hassan
Principal Investigator and
Chairman, Department of Microbiology & Parasitology
Sher-e-Bangla Agricultural University, Dhaka

2. 2
ANNEXURE- A
MINISTRYOF SCIENCE ANDTECHNOLOGY
Government ofthe People’s Republic ofBangladesh
Bangladesh Secretariat, Dhaka – 1000
Tel: 880-2-7164594, Fax: 880-2-7169606
www.most.gov.bd
RESEARCH CONTRACT PROPOSAL
Capacity Utilization Programme
under Special Allocation for Science and
Technology
(Additional Annexure should be submitted wherever necessary)
PART – I: GENERAL INFORMATION
1. NAME AND ADDRESS OF THE CONTRACTING INSTITUTE:
Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
Tel : + 88029180921 Fax: +88028155800 E-mail : kamrullvet03@gmail.com
Mobile: +8801723710845
Money receipt attached (Give Tick or Cross):
2. DEPARTMENT WHERE RESEARCH IS TO BE PERFORMED:
Department of Microbiology & Parasitology, Sher-e-Bangla Agricultural University, Dhaka
1207, Bangladesh
3. TITLE OF THE PROPOSED PROJECT:
Prevalence and antibiotic sensitivity of enterohaemorrhagic Escherichia coli O157: H7
in cattle on smallholdings in Dhaka, Bangladesh.
(A) Name of Coordinated Research Programme (if applicable):
(1) Name and Designation of the authority of the Organization/Institutes /University
forwarding the research contract proposal:
(2) Professor Dr. Alok Kumar Paul, Director, SAURES, Sher-e-Bangla Agricultural
University, Dhaka-1207, Bangladesh
(3) Area of Research: Agriculture/ Medical Science/ Environmental Science/
Biotechnology / Engineering & Applied Science/ ICT/Animal Science/ Aquaculture/
Marine Science/ Microbial and Industrial/ Basic Sciences/ Others (Specify): Animal
Science and Veterinary Medicine (Biology)
4. DURATION (in year): 01 year
5. TOTAL COSTS (in Taka): 22, 52,000 (Twenty Two lac Fifty Two Thousand Tk only)
.


3. 3
PART – 11: INFORMATION ABOUT PROJECT PERSONNEL
1. PROJECT PERSONNEL
A. Principal Investigator
Name
Date of
Birth
Sex:
M/F
Position held (since)
Dr. Md. Kamrul Hassan 01/02/1983 M
Assistant Professor and Chairman,
Department of Microbiology &
Parasitology, Sher-e-Bangla
Agricultural University (April, 2014)
Present Address Permanent Address e-mail Address Mobile
Department of Microbiology
& Parasitology,
Sher-e-Bangla Agricultural
University
Vill: Dhamachama,
Post:Nimgachi, Upazilla:
Dhunat, Dist: Bogra
Kamrullvet03@g
mail.com
01723710845
Academic degreesheld
Subject
Name of
Degree
University Country Class
Year of
Graduati
on
Veterinary
Medicine
MS in
Microbiology
Bangladesh
Agricultural
University
Bangladesh
Grade; A
CGPA: 3.859
(out of 4)
1stclass(77.18%)
2009
Veterinary
Science
DVM
Bangladesh
Agricultural
University
Bangladesh
Grade; A
CGPA: 3.591
(out of 4)
1stclass(71.82%)
2007
Science HSC
Rajshahi
Board
Bangladesh 1st class
(76.70%)
2001
Science
SSC
Rajshahi
Board
Bangladesh 1st class
(79.90%)
1999
Previous experience
1. Assistant Professor and Chairman; From April 10, 2014 to till to date.
Department of Microbiology & Parasitology
Faculty of Animal Science & Veterinary Medicine
Sher-e-Bangla Agricultural University
Dhaka-1207
2. Lecturer ; From 10 April,2012 to 09 April ,2014
Department of Microbiology & Parasitology
Faculty of Animal Science & Veterinary Medicine
Sher-e-Bangla Agricultural University, Dhaka-1207

4. 4
3. Any special Institutionattendedand course taken:
(a) Training on “Operation and Maintenance of Laboratory Equipment” organized by
“Modernization of Animal Husbandry Lab of SAU” sub-project under Higher
Education Quality Enhancement Program (HEQEP) Project, Bangladesh.
(b) Course on “Teaching Methods and Techniques” conducted by the Outreach Program,
Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh.
(c) Course on “Basics of MS Office” organized by Graduate Training Institute(GTI),
Bangladesh Agricultural University, Mymensingh.
(d) Six months professional internship program in different Govt. and non Govt.
organization in Bangladesh.
Publications
(Please provide a complete list of publications in refereed International, regional as well as
national journals):
1. Md. Kamrul Hassan1*, Mahfuzul Islam1, Jayedul Hassan2, J. K. Ghose3, Md. Alimul
Islam2 and Md. Shahidur Rahman Khan. 2014. Isolation and Identification of bacteria
associated with skin lesions of cattle rared in sadar upazilla of mymensingh. International
journal of Bio research: volume 1, issue 4, April, 2014:01-06/Hassan et al.
2. Islam M., Rahman M.M., Khan M.S.R, Hossain M.M. and Hassan M.K. 2011. Culture
sensitivity patterns of bacteria associated with respiratory illness in human. J. Sher-e-Bangla
Agric. Univ., 5(2): 30-35, Bangladesh
3. JK Ghose1,
2 MM Rahman2, MSR Khan3 and MK Hassan3.2011. Life Style of Secondary
School Students in two Selected Schools of Mymensingh Sadar Upazila of Mymensingh
District. Bangladesh J. Environ. Sci., 21: 79-82.
B. Associate Investigator:
Name:
Dr. Md. Abdul Masum
Date of Birth:
01 January, 1985
Sex:
M
Position held (since):
Assistant Professor
Department of Anatomy, Histology &
Physiology, Sher-e-Bangla
Agricultural University, Dhaka-1207
(April 2014)
Present Address
Department of Anatomy, Histology &
Physiology, Faculty of Animal Science &
Veterinary Medicine
Sher-e-Bangla Agricultural University
Dhaka-1207
Bangladesh
E-mail Address: masum_dvmru@yahoo.com
Permanent Address
Vill: Natun huzrapur
P.O: Chapai Nawabganj
P.S: Chapai Nawabganj
District: Chapai Nawabganj

5. 5
Academic degrees:
Subject
Name of
Degree
University Country Class
Year of
Graduation
Veterinary
Medicine
MS in
Anatomy
Bangladesh
Agricultural
University
Bangladesh
First Class
Letter
Grade; A
CGPA: 4
(out of 4)
2011
Veterinary
Science
DVM
Rajshahi
University Bangladesh
First Class
Letter
Grade; A
CGPA: 3.883
(out of 4)
2009
Science HSC
Rajshahi
Board
Bangladesh
First Division
Obtained 70.9%
marks
2002
Science
SSC
Rajshahi
Board
Bangladesh
First Division
Obtained 76.7%
marks
2000
Previous Scientific experience:
1. Assistant Professor; From April 10, 2014 to till to date.
Department of Anatomy, Histology & Physiology
Faculty of Animal Science & Veterinary Medicine
Sher-e-Bangla Agricultural University
Dhaka-1207
2. Lecturer ; From 10 April,2012 to 09 April ,2014
Department of Anatomy, Histology & Physiology
Faculty of Animal Science & Veterinary Medicine
Sher-e-Bangla Agricultural University
Dhaka-1207
Publications:
(Please provide a complete list of publications in refereed International, regional as well as
national journals):
Articles in journals: Total: 10 (National 07 and International 03).
1. M. A. Masum, M. Z. I. Khan, N. H. Siddiqi and M. Nasrin. Frequency of
immunoglobulin containing plasma cells in ileum represents gut-associated-lymphatic
tissues in the BCRDV-vaccinated broiler. Bangl. J. Vet. Med. (2012). 10 (1&2): 15-
20. Bangladesh.

6. 6
2. M. A. Masum, M. Z. I. Khan, M. U. Ahmed, M. N. H. Siddiqi and M. Nasrin.
Histological study of the central or primary lymphoid tissues of broiler chickens. J.
Expt. Biosci. 4(1); January 2013. Bangladesh.
3. M. A. Masum, M. Z. I. Khan and M. U. Ahmed. Histological study of the secondary
lymphoid tissues of broiler chickens. Int. J. Sustain. Agril. Tech. 8(11): 01-04,
November 2012. Bangladesh.
4. M. Nasrin1, M. Z. I. Khan1, 2, M. N. H. Siddiqi1, M. A. Masum1. Mobilization of
immunoglobulin (Ig)- containing plasma cells in Harderian gland, cecal tonsil and
trachea of broilers vaccinated with Newcastle Disease Vaccine. Tissue and Cell 45
(2013) 191– 197. International.
5. Mohd Zahirul Islam Khan, Md. Abdul Masum, Md. Zubayer Ibna Khan, Abdur
Rahman Bin Aziz, Morsheda Nasrin, Mohd Nazmul Hasan Siddique & Mohd
Mokhtar Bin Arshad. Histomorphology of the Lymphoid Tissues of Broiler Chickens
in the Kelantan State of Malaysia. Sains Malaysiana. Accepted. International.
6. N. Sultana, M. Z. I. Khan, M.A. Wares and M. A. Masum. Histomorphological study
of the major lymphoid tissues in indigenous ducklings of Bangladesh. Bangl. J. Vet.
Med. (2011). 9(1): 53 – 58. Bangladesh.
7. M. Nasrin, M. N. H. Siddiqi, M. A. Masum and M. A. Wares. Gross and Histological
study of digestive tract during postnatal growth and development of broiler. Journal of
Bangladesh Agricultural University. Volume 1, Number 10. Bangladesh.
8. M. Nasrin, M. N. H. Siddiqi, M. Z. I. Khan, M. A. Masum and N. Sultana. Gross and
Histological Studies of Harderian Gland and MALT (mucosa associated lymphoid
tissue) of Broilers Vaccinated with BCRDV. International Journal of Bio Research,
Volume 1, Issue 2, February 2011: 1-4. Bangladesh.
9. Nasrin Sultana, Md. Rafiqul Islam and Md. Abdul Masum. Study on the innervaton
of brachial plexus of Black Bengal goat of Bangladesh. International Journal of Bio
Research, Volume 1, Issue 1, March 2011: 1-3. Bangladesh.
10. Md. Abdul Masum, Mohammed Zahirul Islam Khan, Morsheda Nasrin, M. Nazmul
Hasan Siddiqi, Mohammed Zubayer Ibna Khan and Md. Nabiul Islam. Detection of
immunoglobulins containing plasma cells in the thymus, bursa of Fabricius and spleen
of vaccinated broiler chickens with Newcastle disease virus vaccine. International
Journal of Veterinary Science and Medicine 2014. Accepted 2 June 2014.
C. Other Staff [which includes Ph. D/M.Phil/ MS or equivalent degrees (Research) student in
the relevant field] (Applicable for Universities/Institutes which award-academic degrees):
Not Applicable
PART – III: TECHNICAL INFORMATION
1. SCIENTIFIC BACKGROUND OF THE PROJECT:
A. Significance of the proposed research:
Escherichia coli were first described in 1885 by Theodor Escherich (Escherich, 1988). Most
Escherichia coli are harmless commensals which are part of the natural gastrointestinal flora
in the lower intestine of warm-blooded animals. There are over 225 serotypes (unique strains)

7. 7
of E. coli, the majority of which are not dangerous to human health (CIDRAP, 2006).
However, Verotoxin producing Escherichia coli serotype O157:H7 (VTEC) can cause severe
disease on humans than others. On the contrary, animal particularly cattle are their reservoirs
causing no disease there because it does not bind to the walls of their GI (gastrointestinal)
track. In people, E. coli O157:H7 binds to the cells that line our GI tract, Therefore,
Escherichia coli O157:H7 (EC O157) is an important cause of human diarrheal disease.
Severe manifestations include hemorrhagic colitis (i.e. bloody diarrhea) and hemolytic
uremic syndrome (HUS) (Griffin, 1998). Humans can get infected by this organism through
environmental pollution with cattle feces. As it is one of the most important zoonotic
pathogens, during the past 20 years, E.coli O157: H7 has become a world - wide public
health concern. As most cattle in Bangladesh are reared on smallholdings and farmers have
little knowledge about zoonotic disease transmission and how to handle cattle feces for
proper safety. This organism can easily enter into food chain of human to cause serious
disease problem, especially in children.
EC O157 was identified in different areas of the world. The occurrence of E. coli O157:H7
varies in different places of the world. Previous study showed that the prevalence rates
ranged from 0.3 to 19.7% in feedlot cattle and from 0.7 to 27.3% in grazing cattle in The
USA (Hancock et al., 1994), 0.4% of beef carcasses at slaughter and 0.83% of faeces from
live cattle in England and Wales and 15.4% faeces specimens from cattle for slaughter of one
British abattoir are positive to E. coli O157: H7 (Amstrong, 1996). Prevalence rates of E. coli
O157:H7 ranged from 0.3 to 19.7% in feedlots and from 0.7 to 27.3% on pasture have been
reported (Hussein, 2006).
The prevalence of E. coli O157 was also measured in different climatic conditions. In
Scottish beef its prevalence was found to be greater (P < 0.05) during the cooler months (i.e.,
11.2%) than during the warmer months (i.e., 7.5%) (Ogden et al., 2004).
Hussein and Bollinger (2005) reviewed published reports in the past 3 decades and
summarized EC O157 prevalence in beef cattle feces. In general, prevalence rates of E. coli
O157 ranged from 0.3 to 19.7% in feedlot cattle, from 0.7 to 27.3% in cattle on irrigated
pasture, and from 0.9 to 6.9% in cattle grazing on rangeland forages.
The prevalence of VTEC in cattle in Bangladesh has not been demonstrated yet. Because
most cattle in Bangladesh raised in smallholdings and because smallholders have little idea
on its consequences to public health information on VTEC. Prevalence in cattle on
smallholding is to know to mass scale preventive measures to protect human health from the

8. 8
organism. Another problem in Bangladesh is that use antibiotics to treat animal diseases with
suboptimal doses. This practice might have impact in emerging antibiotic resistance. VTEC
strains to zoonotic consequences farther. This study is aimed to examine the prevalence of
VTEC in cattle on smallholdings in Bangladesh and their antimicrobial susceptibility pattern
to some commonly used antibiotic in both medical and veterinary fields in Bangladesh
B. Related work already performed or in progress at the contracting institution / organization
Still there is no work in contracting institute.
C. Related work already performed or in progress at other Institutes in the country (If known)
Very few information are available only for Prevalence and antibiotic sensitivity of
enterohaemorrhagic Escherichia coli O157: H7 in cattle on smallholdings in Dept. of
Microbiology, Chittagong Veterinary and Animal Sciences University, Chittagong.
D. References to important related literature relevant to the project (including own
publication):
1. Amstrong L.G., Hollingsworth J., Glenn Morris J. 1996. Emerging Foodborne
Pathogens: Escherichia coli O157: H7 as a model of entry of a new pathogen
into the food supply of the developed world- Epidemiol. Rev., vol. 18 (1), Pp. 29.
2. Escherich, T. 1988. The intestinal bacteria of the neonate and breast-fed infant.
1884. Rev Infect Dis 10(6), Pp. 1220-5.
3. Griffin, P. M. 1998. Epidemiology of shiga toxinproducing E. coli infections in
humans in the United States. In Escherichia coli O157:H7 and other Shiga toxin-
producing E. coli strains, A. D. O’Brien and J. B. Kaper (Eds.). American Society
for Microbiology, Washington, D.C., Pp. 15–21.
4. Hancock, D. D., Besser, T. E., Kinsel, M. L. P. I. Tarr, D. H. Rice, and M. G.
Paros. 1994. The prevalence of Escherichia coli O157:H7 in dairy and beef cattle
in Washington State. Epidemiol. Infect. Vol. 113. Pp. 199-207.
5. Hussein, H. S., and L. M. Bollinger. 2005a. Prevalence of Shiga toxin-producing
Escherichia coli in beef cattle. J. Food Prot. Vol. 68. Pp. 2224-2241.
6. Hussein, H. S. 2006. Prevalence and pathogenicity of Shiga toxin-producing
Escherichia coli in beef cattle and their products. J Anim Sci. Published online
Oct 23.
7. Ogden, I. D., M. MacRae, and N. J. C. Strachan. 2004. Is prevalence and
shedding of E. coli O157 in beef cattle in Scotland seasonal? FEMS Microbiol.
Lett. Vol. 233. Pp. 297-300.
8. Pablo, N., Stuart, W., Naylor, John, F., et al. 2008. Responses of Cattle to
Gastrointestinal Colonization by Escherichia coli O157:H7. Infection and
Immunity, Vol. 76. Pp. 5366–5372.

9. 9
2. SCIENTIFIC SCOPE OF THE PROJECT
A. Research Objectives:
a) Broad objective: To detect the obscure Pathogenic E. coli O157: H7 from Small
holdings in Bangladesh.
b) Specific objective:
1. To screen the cattle on smallholding for the prevalence of E. coli O157: H7 in
Bangladesh.
2. To examine the sensitivity of E. coli O157:H7 against some commonly used
antibiotic in both medical and veterinary fields in Bangladesh.
3. Geographical distribution and Risk factor will be identified.
B. Relationship of these objectives to the present state of knowledge in the field
C. Research plan including proposed methods or techniques is going to be used
1) Plan of action:
The following plan will be executed to complete the project within one year
 Preparation of questionnaire
 Preparation of Sampling
 Sample Collection sheet
 Body score:
 Sample code:
 Experiment trial on the basis of research objectives
 Collection of fecal sample
 Purchase of research equipments
 Isolation and Identification E. coli O157:H7
 Statistical analysis
 Record keeping
 Analysis of experiments
 Analysis of data
 Report writing
2) Methodology:
Sampling: About 100 cattle smallholding will be randomly selected in an around the Dhaka
district. ≥ 1 cattle heads will be examined in each selected smallholdings. The GPS
coordinates of each selected smallholdings will be noted

10. 10
Collection of fecal sample:
By swab rectal fecal sample will be collected from the animals on selected smallholding.
Microbiological on the collected samples will be conducted at the department of
Microbiology and Parasitology, Faculty of Animal Science and Veterinary Medicine, Sher-e-
Bangla Agricultural University, Dhaka-1207 (SAU).
Isolation and Identification E. coli O157:H7:
With swabs, fecal samples will be collected from the rectal mucosa of cattle (male and
female, various ages). Initial selection of E. coli isolates will be conducted by adding 1 g of
fresh feces from each animal to 9 ML of buffered peptone water (BPW) medium and
incubated at 37 ⁰ C for 5 hours. Then the samples will be streaked on cefixime tellurite
Sorbitol Mac Conkey (CT-SMAC) agar plates, aerobically incubated at 37 ⁰ C for 18-24 h,
and then evaluated for sorbitol fermentation. At the end of this incubation time, sorbitol-
fermenting (i.e., pink colonies) and non-sorbitol fermenting (i.e., white colonies) bacteria on
CT- SMAC plates will be transferred separately into 5-mL tubes containing 2 mL of tryptic
soy broth (TSB) with labeling and incubated at 37 ⁰ C for 6 h with continuous shaking. At
the end of the incubation time, the culture will be diluted with equal volume (i.e., 2 mL) of
sterile glycerol, mixed well, and stored at – 80 ⁰ C. Positive isolates per rectal fecal sample
will be tested for the presence of the rfbO157 gene by using polymerase chain reaction
(PCR). All the presumptive positive E. coli O157: H7 isolates will be tested for susceptibility
to antimicrobial agents.
Statistical analysis: All the collected data will be entered into a spreadsheet program
(Microsoft Excel 2007) and transferred to STATA 9.2 for Windows (Stata Corporation,
College Station, Texas, USA) to determine the prevalence and univariable analysis and
multivariable logistic regression to identify the risk factors associated with the presence of
the pathogen and its resistance to antimicrobials.

11. 11
Sample Collection sheet
Date: Sample code number:
GPS coordinates = X:
Y:
Name of the Place:
Name of the Thana:
Number of cattle in the smallholdings: calf: ………..; Adult: ………….
Clinical presence of any diseases on the day of sample collection:? Yes/No; if yes which
disease suspected?
*Body score: +/++/+++/++++
Any antibiotic used in the past 15 days? Yes/no; if yes which antibiotic:
Duration of antibiotic used:
Is anybody in the family suffering from diarrhea? Yes/no, if yes, duration of illness.
Sample collected from diarrheic person? Yes/no
Sample code: Area/breed/age category/sex/sl.no.
Area = C or N or R; D= Dhaka
Breed type = L or Cb; L= local, Cb= Crossbred
Age category= C or A; C= Calf (≤ 1 year), A= Adult (≥ 1 year)
Sex = M or F
Sl. No. = 1, 2, 3,
Example: C/L/A/F/15
*Body Score: (+) = Good body condition
(++) = Rough body condition and ribs are moderately visible
(+++) = cachectic, protruding rib with prominent pin bone, no diarrhea.
(++++) = cachectic, protruding rib with prominent pin bone, presence of
diarrhea.
Result of the test: Positive / negative

12. 12
3) Time schedule of activities with milestones
Sl.
No.
Name of milestones Starting
date
Completion
date(Approx.)
1 Preparatory work and selection of study area.
Literature review, acquiring equipments and
reagents for methodology, preparing questioner
and data record sheet, taking necessary hazard
precaution and preparation for methodology.
November,
2014
January,
2015
2 Sample collection and laboratory investigation.
Data and sample collection, Isolation and
identification of organism and characterization of
E. coli O157: H7 from positive isolates.
February,
2015
June,
2015
3 Cultural Sensitivity test and data accumulation
CS test of positive sample, all the data will be
accumulated for statistical analysis.
July,
2015
August,
2015
4 Draft report preparation
Report writing
September,
2015
October,
2015
5 Final Report Submission
Submission to the department and funding
authority.
October,
2015
November
2015
Total duration 12 months
D. How is the project related to the stated objective of the Special Allocation for Science and
Technology programme of the GOB?
It is well documented that cattle are the reservoir of E. coli O157: H7 which doesn’t harm in
cattle but it is an important cause of human diarrheal disease. As it is one of the most
important zoonotic pathogens, during the past 20 years, E.coli O157: H7 has become a world
- wide public health concern. The prevalence of VTEC in cattle in Bangladesh has not been
demonstrated yet. This study is very important to determine the prevalence in cattle on
smallholding to know to mass scale preventive measures to protect human health from the
organism and to conduct CS test to minimize the impact of emerging antibiotic resistance
which is highly relevant in Bangladesh context. Moreover, the output of this research is
directly related to the aim and objectives of DLS that is enhancing research and collaboration
between private and public stakeholders. If a baseline data on prevalence of E. coli O157 in
smallholdings are established, we can disseminate it to public health worker and farmers
through DLS.
E. How is the programme related to academic degree programme? (If applicable) (Only for
universities/Institutes which awarded degree): Not applicable
F. What outputs from the project can be considered for the assessment of its success?
This research might reveal the existence of E. coli O157: H7 on cattle of small holdings in
Bangladesh will be determined. These findings will be very important for further research
regarding to disease control in Bangladesh as well as less biological hazard for the mankind.

13. 13
G. How does the project contribute in the development of sustainable technology?
If we own the project it will enhance the lab basis education, specially it will be very helpful
in molecular diagnosis, which are now the base of accurate disease diagnosis. Above all, both
Ms and undergraduate student will avail the opportunity of using modern disease diagnostic
equipments.
3. LIST OF FACILITIES AVAILABLE (Equipment and other facilities including
laboratory space)
Laboratory with few equipments
PART – IV: BUDGET INFORMATION
1. BUDGET
A. Current year:
Item Cost (thousand Taka) Taka (Thousand)
1. Minor equipment 4,00.00
2. Spare parts for major equipment 9,00.00
3. Consumables including chemicals, books, software etc. and sample
collection expenses
3,00.00
4. Other essential expenses (maximum one fifth of the total budget) 652,00.00
Total- Twenty Two lac Fifty Two Thousand Tk only
B. If the project is expected to last more than one year, please include budget estimates
for the total period: Not applicable
Item Cost (thousand Taka)
1st year 2nd year 3rd year
1. Minor equipment
2. Spare for major equipment
3. Consumables
4. Other essential expenses
Total
* Detailed list of items with approximate price must be included.

14. 14
PART – IV: BUDGET INFORMATION
1. BUDGET
A. Current year:
Item Cost (in taka)
1. Minor equipment 4,00,000.00
2. Spare parts for major equipment 9,00,000.00
3. Consumables including chemicals, books,
software etc. and sample collection
expenses
3,00,000.00
4. Other essential expenses 6,52,000.00
Total 22,52,000.00
Total- Twenty Two lac Fifty Two Thousand Tk only
B. If the project is expected to last more than one year, please include budget estimates
for the total period: Not applicable
* Detailed list of items with approximate price included.
Detailed Proposed budget (in Taka)
Sl Items Cost ('Tk)
1 Minor Equipment
Computer, printer, scanner, UPS,Volt Stabilizer, Computer table
Glass wares and equipments
Conical flask
Measuring cylinder
Postmortem box
Slide & slide box
Inoculating loop
Morter & pastle
Collection vial
Petridishes
Bunsen burner
Syringe & needle
Test tube
Beaker
Mortar and pastle
200000.00
200000.00
Sub Total 4,00,000.00
2 Spare parts for major equipment
Parts for PCR machine and Gel documentation systems, Incubator,
Centrifuge Machine( High speed), Freeze, Dry air Oven, Hot water
bath,
900000.00
Sub Total 9,00,000.00

15. 15
3 Consumables Expenses
Chemicals(10%formalin.,70% ethanol, absolute alcohol.5%propanol,
nutrient agar media , selenite broth, EMB agar media, Blood agar media,
Macconkey agar media)
Reagents (crystalviolet stain, eosin stain, nigrosin stain, giemsa stain,
malachite green stain, phosphate buffer solution), primer for PCR,
antibiotic discs
300000.00
Sub Total 3,00,000.00
4 Other Essential Expenses
Traveling (Data collection and farm visit)
Questionnaire preparation and printing
Paper Purchase
Photocopy
Signboard, visualization and documentation
Binding
Report writing
Stationary
Other Utilities
Publication and printing
Honourium for PI (1; @ Taka 22000.00/ year) one month basic
Honourium for Co PI (1; @ Taka 22000.00/ year) one month basic
Research Fellow (2; @ taka 10000/month)
Guard (1; @ 5000.00 TK / month)
Labors (1 person 180.00 TK/day, 300 days)
100000.00
50000.00
50000.00
22000.00
22000.00
240000.00
60000.00
54000.00
Sub Total 6,52,000.00
Grand Total 22,52,000.00
2. STATUS OF LEGAL PERSONALITY AND ACCOUNTING SYSTEM
As per GOB rule
PART – V: PREVIOUS FUNDING INFORMATION UNDER SPECIAL ALLOCATION
FROM M/O SCIENCE AND TECHNOLOGY (MOST)
1. Did you get any funding under special allocation from MOST since 1997 – 98?
Yes No
(If your answer is no please escape the following sections)
2. Funding year:
3. Amount of fund (in Taka):
4. What was the title of the project?
5. Project is completed or not?
6. If not what is the expected date of completion?


16. 16
7. Already submitted working report or scientific report or not?
8. Expected date of submission of Scientific Report?
9. Any paper published in any International, regional / local journal from this research?
10. Quote the name of the journal, date of publication and title of the paper.
PART – VI: DECLARATION/CERTIFICATION
It is certified that –
(a) The same project has not been submitted to any other agency / agencies for financial support.
(b) The research work proposed in this project is not a duplicate work already done or being done in
the field (i.e. area of research)
(c) We agree to accept the terms and conditions developed for the Special Allocation for Science and
Technology as mentioned in the Guidelines.
(d) Associate Investigator assures the responsibility of the Project in case the Principal Investigator
leaves the institution/Organization.
(e) Project will be provided with access to all available facilities in the Organization.
Signature and Name of the
Principal Investigator
(With seal, Telephone number & Mobile number)
Signature and Name of the Head of the Organization /
Institutes /University
(With seal, Telephone number & Mobile number)
Signature and Name of the Associate Investigator:
(With seal, Telephone number & Mobile number)