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Kaumil Bhavsar
Kaumil Bhavsar

This document discusses the basics of cell culture media and its supplements. It describes the key components of media including nutrients, non-nutrients, and serum. Serum provides many benefits as a medium supplement but also has disadvantages like introducing animal viruses and variability between batches. Defined serum-free media are now commonly used to avoid these issues. The choice of medium and its constituents can impact the culturing and passaging of cells.

TechnologieSanté & Médecine
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Basics of Cell Culture Media & its Supplements - Ravin Mehta
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[object Object],High M.W Substances Metabolites Nutrient Substances Metabolites Lipids Anti Oxidants Protective Agents Buffers Antibiotics Non Nutrient Substances Trace Elements Bulk Ions Vitamins Nitrogen Sources Energy Sources Water
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[object Object],[object Object],[object Object],[object Object],Important for methylation, degradation of amino acids & fattyacids Vitamin B12 Carrier of CO 2 , required for functioning of pyruvate dehydrogenase, pyruvate carboxyase & fatty acid synthesis Biotin Catalyses transamination Pyridoxal phosphate Catalyst in the transfer of carboxyl group, transaldolases, transketolases Thiamine pyrophosphate Regulation of Ca 2+ , important to maintain at appropriate levels. Vitamin D Antioxidant Vitamin E Many Vitamin K dependent proteins Vitamin K Growth & differentiation of some cells Vitamin A Synthesis of collagen Ascorbic acid
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