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Potassium Solublising Micro organisms
FROM,
koushalya t.n
jr.Msc(Agri) in
Agronomy
3
Introduction
 Potassium is the most abundant macronutrient in
soils.
 It is also the seventh most common element in the
lithosphere which contains on average 2.6%
potassium.
 The total potassium content of Indian soils varies
from 0.5 to 3.0%.
 FORMS OF SOIL K
6
 There are four forms of K in soil:
1. Solution K
2. Exchangeable K
3. Fixed or non exchangeable K
4. Structural or mineral K
Why ...........KSB......?
8
 Potassium fixation : It is define as the
conversion of soil solution or exchangeable
K into nonexchangeable forms and was once
considered a negative soil property causing
a drastic reduction of plant available K.
The Potassium Solubilizing
Microorganisms (Ksms)
Rhizospheric
microorganism
which solubilizes
the insoluble K to
soluble forms of K
• Bacillus mucilaginosus,
• B. edaphicus,
• B. circulans,
• Acidothiobacillus ferrooxidans,
• Paenibacillus
• Aspergillus spp.
• Aspergillus terreus
Main mechanism of ksms
• Acidolysis,
• Chelation,
• Exchange Reactions,
• Complexolysis
• Production of organic acid.
• OM after decomposition produces acids like
citiric acid, formic acid, malic acid, oxalic acid.
These organic acids produced, enhance the
dissolution of potassium compounds by supplying
protons and by complexing Ca2+ ions .
• OM produced by micro-organisms such as
acetate, citrate and oxalate can increase mineral
dissolution in soil
• Solubilization of potassium occurs by complex
formation between organic acids and metal ions
such as Fe2+, Al 3+ and Ca2+
Screening Of Potassium Solublising
Microorganisms
• Potassium solubilizing
microorganisms can be
isolated by serial
dilution method using
Aleksandrov medium
Aleksandrov medium
1% glucose,
0.05% MgSO4.7H2O,
0.0005% FeCl3,
0.01% CaCO3,
0.2% CaPO4 and
0.5% potassium aluminium
silicate
6.5 pH-6.5
Frateuria aurantia
• Scientific classification
• Domain:Bacteria
• Phylum:Proteobacteria
• Class:Gammaproteobacteria
• Order:Xanthomonadales
• Family:Xanthomonadaceae
• Genus:Frateuria
• Species:F. aurantia
Introduction
• K Sol B® is a biological fertilizer based on a
selected strain of naturally-occurring
beneficial proteo bacteria Frateuria aurantia
• Frateuria aurantia is used as an effective soil
inoculant.
• K Sol B® contains vegetative cells of Frateuria
aurantia .
• It is formulated as Wettable Powder with CFU
count of 5 X 10 ^ 7 / g.
History
• Joseph Frateur
• Frateuria aurantia was
isolated from the plant
Lilium auratum and
from the fruit of the
raspberry Rubus
parvifolius.
Mode of Action
• Carbon utilization: The bacteria
contained in K Sol B® on
application to the soil get
activated and multiply by utilizing
the carbon source of soil or
exudates of the root and in this
process secrete organic acids and
enzymes.
• Metabolite production:
Frateuria aurantia produces
organic acids and enzymes
that help solubilize the fixed
potassium into
exchangeable form and
make it assimable by plants.
Method of Application
• Seed Treatment: Mix 10 g. of K Sol B® with 10
g. of crude sugar in sufficient water to make a
slurry and coat 1 kg of seeds.
• Dry the seeds in shade and sow / broadcast /
dibble in the field.
• Seedling treatment: Mix 100 g. of K Sol B®
with sufficient quantity of water and organic
manure to form a slurry.
• The seedlings are dipped in this slurry for 30
minutes prior to planting so that the bacteria
get attached to the roots.
• Soil application: Mix 3-5 Kg/ acre of K Sol B®
with compost and apply to an acre of soil.
• Drip Irrigation : Mix 3 Kg/ acre of K Sol B® in
drip stream.
• Compatibility K Sol B® is compatible with
BioPesticides and other BioFertilizers.
• Shelf Life K Sol B® is stable for a period of 1
year from the date of manufacturing.
Crops
• K Sol B® is suitable for application on Cereals ,
Millets , Pulses, Oilseeds, Fibre Crops , Sugar
Crops , Forage Crops , Plantation crops
,Vegetables, Fruits, Spices , Flowers , Medicinal
crops , Aromatic Crops , Orchards and
Ornamentals.
• OTHER FORMULATIONS AVAILABLE
• Frateuria aurantia CFU/ml 1*10^8 Liquid
• Frateuria aurantia CFU/g/ml *10^9 Powder/
Liquid
• Frateuria aurantiaCFU/g 1*10^10 Powder
Cautions
1. Mixing equipment is to be thoroughly rinsed with
water and detergent before using the same
equipment for formulating other fertilizers/
pesticides.
2. Surplus product may be disposed in crop lands
3. Do not eat / drink / smoke during application
4. Direct incidence of K Sol B® may cause irritation
and therefore it is recommended that the
operator should use protective gear viz gloves,
apron, mask, eye gear and hood.
Commitment to Nature
organic agriculture.
Safe to use along with bio fertilizer
Can be used as an effective component in
ipm/ inm programmes
Advantages
• Effectively mobilize unavailable potassium
ions and make it assimable by plants
• natural potassium solubilization improves both
plant and soil health and also aids in soil
remediation.
• The increase in the beneficial microbe
population in soil improves soil health.
• Earthworm friendly ,
• Eco friendly
Potassium  solublising micro organisms

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Potassium solublising micro organisms

  • 2. Potassium Solublising Micro organisms FROM, koushalya t.n jr.Msc(Agri) in Agronomy
  • 3. 3 Introduction  Potassium is the most abundant macronutrient in soils.  It is also the seventh most common element in the lithosphere which contains on average 2.6% potassium.  The total potassium content of Indian soils varies from 0.5 to 3.0%.
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  • 6.  FORMS OF SOIL K 6  There are four forms of K in soil: 1. Solution K 2. Exchangeable K 3. Fixed or non exchangeable K 4. Structural or mineral K
  • 8. 8  Potassium fixation : It is define as the conversion of soil solution or exchangeable K into nonexchangeable forms and was once considered a negative soil property causing a drastic reduction of plant available K.
  • 9. The Potassium Solubilizing Microorganisms (Ksms) Rhizospheric microorganism which solubilizes the insoluble K to soluble forms of K
  • 10. • Bacillus mucilaginosus, • B. edaphicus, • B. circulans, • Acidothiobacillus ferrooxidans, • Paenibacillus • Aspergillus spp. • Aspergillus terreus
  • 11. Main mechanism of ksms • Acidolysis, • Chelation, • Exchange Reactions, • Complexolysis • Production of organic acid.
  • 12. • OM after decomposition produces acids like citiric acid, formic acid, malic acid, oxalic acid. These organic acids produced, enhance the dissolution of potassium compounds by supplying protons and by complexing Ca2+ ions . • OM produced by micro-organisms such as acetate, citrate and oxalate can increase mineral dissolution in soil • Solubilization of potassium occurs by complex formation between organic acids and metal ions such as Fe2+, Al 3+ and Ca2+
  • 13. Screening Of Potassium Solublising Microorganisms • Potassium solubilizing microorganisms can be isolated by serial dilution method using Aleksandrov medium
  • 14. Aleksandrov medium 1% glucose, 0.05% MgSO4.7H2O, 0.0005% FeCl3, 0.01% CaCO3, 0.2% CaPO4 and 0.5% potassium aluminium silicate 6.5 pH-6.5
  • 15. Frateuria aurantia • Scientific classification • Domain:Bacteria • Phylum:Proteobacteria • Class:Gammaproteobacteria • Order:Xanthomonadales • Family:Xanthomonadaceae • Genus:Frateuria • Species:F. aurantia
  • 16. Introduction • K Sol B® is a biological fertilizer based on a selected strain of naturally-occurring beneficial proteo bacteria Frateuria aurantia • Frateuria aurantia is used as an effective soil inoculant. • K Sol B® contains vegetative cells of Frateuria aurantia . • It is formulated as Wettable Powder with CFU count of 5 X 10 ^ 7 / g.
  • 17. History • Joseph Frateur • Frateuria aurantia was isolated from the plant Lilium auratum and from the fruit of the raspberry Rubus parvifolius.
  • 18. Mode of Action • Carbon utilization: The bacteria contained in K Sol B® on application to the soil get activated and multiply by utilizing the carbon source of soil or exudates of the root and in this process secrete organic acids and enzymes.
  • 19. • Metabolite production: Frateuria aurantia produces organic acids and enzymes that help solubilize the fixed potassium into exchangeable form and make it assimable by plants.
  • 20. Method of Application • Seed Treatment: Mix 10 g. of K Sol B® with 10 g. of crude sugar in sufficient water to make a slurry and coat 1 kg of seeds. • Dry the seeds in shade and sow / broadcast / dibble in the field.
  • 21. • Seedling treatment: Mix 100 g. of K Sol B® with sufficient quantity of water and organic manure to form a slurry. • The seedlings are dipped in this slurry for 30 minutes prior to planting so that the bacteria get attached to the roots.
  • 22. • Soil application: Mix 3-5 Kg/ acre of K Sol B® with compost and apply to an acre of soil. • Drip Irrigation : Mix 3 Kg/ acre of K Sol B® in drip stream.
  • 23. • Compatibility K Sol B® is compatible with BioPesticides and other BioFertilizers. • Shelf Life K Sol B® is stable for a period of 1 year from the date of manufacturing.
  • 24. Crops • K Sol B® is suitable for application on Cereals , Millets , Pulses, Oilseeds, Fibre Crops , Sugar Crops , Forage Crops , Plantation crops ,Vegetables, Fruits, Spices , Flowers , Medicinal crops , Aromatic Crops , Orchards and Ornamentals.
  • 25. • OTHER FORMULATIONS AVAILABLE • Frateuria aurantia CFU/ml 1*10^8 Liquid • Frateuria aurantia CFU/g/ml *10^9 Powder/ Liquid • Frateuria aurantiaCFU/g 1*10^10 Powder
  • 26. Cautions 1. Mixing equipment is to be thoroughly rinsed with water and detergent before using the same equipment for formulating other fertilizers/ pesticides. 2. Surplus product may be disposed in crop lands 3. Do not eat / drink / smoke during application 4. Direct incidence of K Sol B® may cause irritation and therefore it is recommended that the operator should use protective gear viz gloves, apron, mask, eye gear and hood.
  • 27. Commitment to Nature organic agriculture. Safe to use along with bio fertilizer Can be used as an effective component in ipm/ inm programmes
  • 28. Advantages • Effectively mobilize unavailable potassium ions and make it assimable by plants • natural potassium solubilization improves both plant and soil health and also aids in soil remediation. • The increase in the beneficial microbe population in soil improves soil health. • Earthworm friendly , • Eco friendly