It is a presentation in detail about the strongest structure of the oral cavity "ENAMEL". It is a simple topic but people find it difficult to learn about it. I hope my presentation is a simple method to learn about it. I would like to thank my professors for assign me this project and i learn't a lot from it and still learning my basics daily.
7. INTrOduCTION
ENAMEL is an epithelially derived protective covering of
variable thickness over the entire surface of the crown.
•It is the hardest biologic tissue in the body.
•It attains the maximum thickness of about 2-2.5mm on the
cusps of molars and premolars.
•It thins down to almost knife edge at neck of the tooth.
8. PHYSICAL PROPERTIES:
1. Thickness
• 2.5mm (thickest) Over cusp of molar
• 2.0 mm over incisal edge
• Knife edge thickness at cervical region
• Thick at maxillary lingual surfaces of
molars and mandibular buccal surfaces.
9. 2. Color
• Depends on thickness and translucency
of enamel.
• Grayish white to yellowish white.
• Yellowish - At thin areas underlying
dentin.
• Grayish – Thick opaque enamel
• Bluish – Incisal area
10. 3. Hardness
• 296 (Knoop’s hardness no.)
• It is the hardest structure in the body.
• Peripheral region are more harder.
4. Solubility
• Dissolves in acidic media.
11. 5. Permeability
•Enamel is selective permeable.
•Route of passage occurs via rod
sheath, enamel lamellae and enamel
tufts which are rich in organic
content.
6. Specific gravity: 2.8
7. Translucency: Semi translucent.
15. HYPOCALCIFIED AREAS OF ENAMEL
o Rod sheath
o Incremental lines of Retzius
o Enamel lamellae
o Enamel tufts
o Enamel cracks
o Enamel spindles
o Neonatal line
16. ENAMEL RODS
Shape- Cylindrical
Starts from DEJ to outer enamel surface
Number-5 million in lower lateral
incisor to 12 million in upper 1st
molar.
Course-Tortuous from DEJ.
17. •Length- greater than thickness of enamel.
•Diameter: 4 µm in light microscopy.
It increases from DEJ to outer
enamel surface by a ratio of 1:2 since
outer surface of enamel is greater than
inner surface.
•Appearance-Clear crystalline.
18. Light microscopy:-
Rods appear hexagonal.
In cross section : Fish scale
appearance.
Recently – Arcade outline near
DEJ and keyhole outline at
enamel surface.
SIGNIFICANCE
Because of inter-woven
network of rods, teeth can
resist masticatory forces up to
20-30 pounds per tooth.
20. ULTRASTRUCTURE/
ELECRONMICROSCOPY
Shape of rod: Key
hole/Paddle shaped prisms
Measurement:
9 µm – length
5 µm – breadth
“Bodies” of rods – near
occlusal/incisal surface and
“Tails” point cervically.
21. HYDROXYAPATITE CRYSTALS
• Arranged approximately parallel to the long axis of the rods.
• Length – 0.05 to 1µm.
• Width - 90µm.
• Pyramid shape.
22.
23. • A thin peripheral layer.
• Darker than the rod.
• Relatively acid-resistant.
• Less calcified and contains more organic matter
than the rod itself.
• Electron Microscope : often incomplete.
25. • Cementing E. rods together.
• More calcified than the rod sheath.
• Less calcified than the rod itself.
• Appears to be minimum in human teeth.
26. STRIATIONS
•Enamel rods is built up of
segments of uniform length of
about 4 um, separated by dark
lines that give it a striated
appearance.
•More visible by the action of
mild acids.
•Appearance is because of
formation of enamel matrix in
rhythmic manner.
•More pronounced in hypo
calcified areas.
27. DIRECTION OF RODS
•Rods are oriented at right
angles to the dentinal surface.
•In the cervical and central
parts of crown of the deciduous
teeth, they are approximately
horizontal.
•Near incisal edge and tip of the
cusps they change gradually to
an increasingly oblique
direction until they are almost
vertical in the region of the
edge or tip of cusp.
Fig-Deciduous tooth
28. •In the permanent teeth,
arrangement of rods is similar
to deciduous teeth in occlusal
2/3 of crown.
•In the cervical region rods
deviate from the horizontal to
apical direction.
•Alternative clockwise and
counterclockwise
alteration/deviation of the rods
from the radial direction can be
observed at all levels.
Fig-Permanent Tooth
29. •In deciduous teeth, direction of rods is
horizontal in cervical and central parts of the
crown. Near incisal edge or tip of cusp they
gradually increase in oblique direction and
almost vertical in the cusp tip region.
• In permanent teeth, in occlusal two third of
the crown direction of rods is oblique .
• In cervical region rods deviate from the
horizontal in apical direction.
30. GNARLED ENAMEL
•Near the dentin in the region
of cusps or incisal edge,
bundles of rods seem to
interwine more irregular,
especially in section cut
obliquely. This optical
appearance of enamel is
called gnarled enamel
•In addition, enamel rods
converge in outward course,
in pits and fissures occlusal
surface of molars and
premolars.
31. •In this section, we can see the
end of an odontoblastic process
penetrating the enamel and just
pass the DEJ.
This structure is called an
enamel spindle.
Fig:- A- Gnarled enamel
B- Enamel spindle
32. HUNTER-SCHREGER
BANDS
•The regular change in the
direction of rods is responsible
for the appearance of alternating
dark and light strips of varying
width which are called as
Hunter Schreger bands.
•Can be seen in longitudinal GS
under oblique reflected light.
•HS bands originate at DEJ &
pass outwards ending in some
distance from outer enamel
surface.
33. Possible causes:-
•Variation in
calcification process.
•They may not be an
optical phenomena
but they are composed
of alternate zones
having slightly
different permeability
and different content
of organic material.
36. INCREMENTAL LINES
OF RETZIUS
•Brownish bands seen in
ground sections of enamel that
illustrate successive apposition
of layers of enamel during
formation of the crown are
called Incremental lines of
Retzius.
In Longitudinal Section,
They surrounds tip of dentin
From the DEJ. In cervical parts
they run obliquely, deviate to
occlusal.
37. In TranverseSection,
•They appear as concentric
circles
•It has been attributed to
•periodic bending of enamel
rods.
•Variation in basic organic
structure.
•Physiologic calcification
rhythm.
SIGNIFICANCE
•Broadening of Incremental
lines may reflect metabolic
disturbance at the time of
matrix formation.
41. •STRUCTURELESS LAYER
•About 30 µm thick, called prismless enamel
•In 70% permanent teeth and all deciduous teeth.
•Found least often over the cusp tips.
•Found commonly in the cervical areas.
•No Enamel prisms visible.
•All the apatite crystals are parallel to one another and
perpendicular to the striae of Retzius.
•More mineralized than the bulk of Enamel beneath it.
43. PERIKYMATA
•These are transverse wave like
grooves, believed to be the
external manifestations of striae
of Retzius.
• 30 Perikymata per/mm in the
region of CEJ in number.
•Their concentration gradually
decreases near occlusal or incisal
surface to about 10 per/mm.
44. Fig- Ground section of enamel showing the relationship
between the striae of Retzius and surface Perikymata .
45. Fig- Scanning electron micrograph of the labial
surface of a tooth, showing the Perikymata.
46. ENAMEL ROD ENDS
•These are concave and vary in depth.
•They are shallow cervically and deep
occlusally/incisally.
•Pits of about 1-1.5µm in diameter and small
elevations of about 10-15µm are called enamel
caps.
•Larger enamel elevations are called enamel
brochs.
47. CRACKS
•They are actually outer edges of lamellae.
•Extent:-
They originate from incisal edge & extend to
varying distances in enamel in perpendicular
direction towards dentinoenamel junction.
•Length: 1mm mostly.
49. NEONATAL LINE
•The enamel of the deciduous teeth develops partly before
& partly after birth. The boundary between the 2 portions of
enamel in the deciduous teeth is marked by an accentuated
Incremental line of Retzius, the Neonatal line/ring.
•Results from abrupt change in the environment & nutrition
of newborn infant.
•Prenatal enamel is usually better developed than postnatal
enamel as fetus develops in well protected environment
with an adequate supply of all essential materials.
•Perikymata are absent in prenatal enamel.
51. ENAMEL CUTICLE:
•A delicate membrane covers the crown of newly erupted
tooth called Nasmyth’s membrane or primary enamel cuticle.
This is soon removed by mastication.
•This is secreted after epithelial enamel organ retracts from
cervical regions during tooth development.
•It protects the surface of enamel from resorptive activity of
adjacent vascular tissue.
52. • Covers the entire crown of newly erupted tooth.
• Thickness: 0.2 µm.
• Removed by mastication (remains intact in protective
areas).
• Secreted by postamloblasts.
• EM: similar to basal lamina.
53. Covered the cervical area of the enamel.
Thickness: up to 10 µm.
Continuous with the cementum.
Probably of mesodermal origin or may be
elaborated by the attachment epithelium.
Secreted after E.O. retracted from the cervical
region during tooth development.
54. PELLICLE:
•Erupted enamel is covered by a precipitate of salivary
proteins called pellicle.
•This pellicle reforms within hours after mechanical
cleaning.
•It becomes colonized by microorganisms within a day or
two after formation which forms bacterial plaque.
55. ENAMEL LAMELLAE:
•Thin leaf like structures that
extend from enamel surface
toward DEJ.
Composition mainly organic, little
mineral.
Origin:-
•Develops in planes of tension.
when rods cross such a plane ,
they may not fully calcify.
•If the disturbance is more severe,
a crack may develop.
•Crack is filled either by
surrounding cells if it has
occurred in unerupted tooth,Or by
organic material if it has occurred
after eruption.
56. TYPES
Type A : Consists of poorly calcified
rod segments.
Restricted to enamel.
Type B: Consists of degenerating cells.
May reach into dentin.
Type C: Containing organic material,
presumably from saliva.
If connective tissue invades the cracks
in enamel, cementum may be formed.
57. SIGNIFICANCE:-
It has been suggested that lamellae may be a site of
weakness in a tooth and may form a road of entry for
bacteria that initiate caries.
Enamel
Enamel lamellae
Dentin
58. ENAMEL TUFTS:
•Thin ribbon like structure, resembling tufts of grass which is
created by examining such area under low magnification in
thick ground section.
•Tufts consists of hypo calcified enamel rods & interprismic
substance.
•The major organic component of tufts was 13.17 kd protein.
59. •They arise at DEJ &
reach in to enamel to
about 1/5 to 1/3 of its
thickness.
•Their presence & their
development are
consequence of an
adaptation to spatial
condition of enamel.
SIGNIFICANCE
Enamel tufts prevents
enamel fractures.
60. DENTINOENAMEL JUNCTION
•Scallope structure-The surface of the dentin at DEJ is
pitted, in shallow depression of dentin, fit rounded
projection of enamel.
•It appears scalloped due to the mixing of crystals of dentin
and enamel with each other.
62. ENAMEL SPINDLES
•Occasionally Odontoblast
processes pass across DEJ
into enamel, since many
are thickened at their end,
they have been termed
enamel spindles.
•Directions of spindles and
rods are divergent as rods
are formed at right angle to
ameloblast and spindles are
parallel to ameloblasts.
64. DEVELOPMENT OF ENAMEL
• At advanced bell stage preceding the formation of hard
structure (dentin and enamel), the enamel organ consists
of 4 distinct layers.
1. Outer enamel epithelium
2. Stellate reticulum
3. Stratum intermedium
4. Inner enamel epithelium
65. OUTER ENAMEL EPITHELIUM
•It consists of single layer of cuboidal cells.
•On highest convexity of organ cells of OEE become
irregular in shape.
•The capillaries in the connective tissue surrounding
epithelial enamel organ proliferate and protrude
towards it.
•Immediately before enamel formation commences
capillaries may indent stellate reticulum. This
increased vascularity ensures rich metabolism when a
plentiful supply of bloodstream to the inner enamel
epithelium is required.
66. •During enamel formation, the cells of OEE
develop Villi, cytoplasmic vesicles and large
number of mitochondria all indicating
specialization for active transport of material.
•Capillaries adjacent to OEE are thin walled
indicating active transport.
67. STELLATE RETICULUM
• Middle part of enamel organ.
• Consists of cells that are star shaped with long
processes reaching in all directions from central body.
Neighboring cells are separated by wide intracellular
spaces filled by large amount of intracellular fluid.
• The cells are connected with each other and to the cells
of stratum intermedia and OEE by desmosomes.
68. FUNCTION OF STELLATE RETICULUM
• Elasticity and resistance: it acts as buffer against
physical forces that might distort developing
DEJ, giving rise to gross morphology.
• It permits only limited flow of nutritional
elements from overlying blood vessels to
formative cells.(when the first layer of the
dentin is laid down, it collapses to bring the
blood vessels nearer.)
69. Reason for characteristic morphology
•The cells in the center of e o continue to
synthesize and secrete glycosaminoglycans
• into the extra cellular compartment between
the epithelial cells
• Hydrophilic and pull water in to dental organ
• Increases the volume of extra cellular
compartment,
• Cells are forced apart maintaining
connections with each other through
desmosomal contacts,
• Creating star shape called stellate reticulum.
70. STRATUM INTERMEDIUM
•Flat or cuboidal cells.
•Situated between stellate reticulum & OEE.
•Function:
It is not fully understood, but it is believed to play
role in production of enamel itself, either by control
of fluid diffusion into and out of ameloblast or by
the actual contribution of necessary formative
elements or enzymes.
71. INNER ENAMEL EPITHELIUM
• Derived from basal cell layer of oral
epithelium.
• Before enamel formation begins, these cells
assume a columnar form and differentiate into
ameloblasts that produces enamel matrix.
• Functions:
1. Determining crown pattern.
2. Induces differentiation of Odontoblasts from
cells of dental papilla ??????
3. Enamel formation after differentiation into
ameloblasts.
72. CERVICAL LOOP
•At the border of wide
basal opening of enamel
organ, inner enamel
epithelium reflects on outer
enamel epithelium. This is
called Cervical Loop.
Function: When the crown
has been formed, the cells
of this portion give rise to
Hertwig’s epithelial root
sheath.
73. LIFE CYCLE OF AMELOBLASTS
• According to function the life span of the cells of the inner
enamel epithelium can be divided into six stages:
Morphogenic stage
Organizing stage
Formative stage
Maturative stage
Protective stage
Desmolytic stage
74. The various functional stages in
the life cycle of the cells of the
inner enamel epithelium.
I. Morphogenetic stage
II. Histodifferentiation
stage
III. Initial secretory stage
(no Tomes’ process)
IV. Secretory stage (Tomes’
process)
V. Ruffle-ended ameloblast
of the Maturative stage
VI. Smooth-ended
ameloblast of the
Maturative stage
VII.Protective stage.
75. MORPHOGENIC STAGE:
•Before ameloblasts are fully differentiated IEE interacts
with adjacent mesenchymal cells determining shape of
the DEJ and the crown.
•During this stage, cells are short, columnar with large
oval nuclei that almost fills the body.
Morphogenic
stage
76. MORPHOGENIC STAGE
•Golgi apparatus and centrioles are located in the proximal end of
the cells and mitochondria are evenly dispersed throughout
cytoplasm.
•Terminal bars appear concomitantly with the migration of
mitochondria to basal region of cells. Terminal bars represent
point of close contact between cells.
•They are believed to be thickening of apposing cell membrane
associated with condensation of under lying cytoplasm.
•Basal lamina separates IEE from CT.
•There is presence of cell free zone consisting of argyrophilic
fibers and cytoplasmic processes of superficial cells of pulp.
77. ORGANISING STAGE
•Change in the appearance of cells of IEE. They become longer.
•Reversal of functional polarity takes place by migration of
Golgi and centrioles to distal ends and mitochondria to proximal
ends.
•Disappearance of cell free zone and so they come in contact
with dental papilla cells and induce their differentiation into
Odontoblasts.
•In late or terminal stage odontoblasts begin to secrete dentin
which is a critical phase in life cycle of IEE, it differentiates into
ameloblast.
78. •Along with this ameloblasts are cut off from their
original source of nutrition that is connective tissue
of dental papilla, which is compensated by dental
sac.
• Proliferation of capillaries adjacent to OEE.
• Reduction and gradual disappearance of stellate
reticulum, thus minimizing the distance between
capillaries.
79. FORMATIVE STAGE
•First layer of dentin is
necessary for beginning of
enamel matrix formation.
Secretary stage
(no Tomes process)
80. •During formation of enamel
matrix earliest apparent change
is development blunt cell
processes on ameloblast
surfaces, which penetrate the
basal lamina and enter predentin
(Tomes process).
Secretary stage
(Tomes process)
81. MATURATIVE STAGE
•Enamel maturation occurs after
most of the thickness of enamel
has been formed in occlusal or
incisal area.
•During this phase
Ameloblasts are slightly
decreased in lengt
•And closely attach to enamel
matrix.
•They display microvilli at distal
end.
Ruffle ended ameloblasts of
maturative stage
83. •Stratum intermedium cells
loose their cuboidal shape and
regular arrangement and
assume spindle shape.
•In enamel maturation
ameloblast are involved in
cyclic process in which
organic material is removed.
•Inorganic material is
introduced and this process is
reflected on morphology of
the cells.
Smooth ended ameloblasts
of maturative stage
84. PROTECTIVE STAGE
•When enamel has completely developed and fully calcified.
•The ameloblast cease to be arranged in a well defined layer
can no longer be differentiated from cells of stratum
intermedium and OEE.
•These cell layers forms a stratified epithelial covering of the
enamel, called reduced enamel epithelium.
85. DESMOLYTIC STAGE
•The reduced enamel epithelium proliferates and induces
atrophy of the CT separating it from oral epithelium so that
fusion of 2 epithelia can occur.
•It is probable that REE cells elaborate enzymes that are
able to destroy CT fibers by desmolysis.
Significance
•Premature degeneration of REE may prevent eruption of
tooth.
86. Function of Reduced Enamel Epithelium
To protect the mature enamel from degeneration until tooth
erupts.
87.
88. AMELOGENESIS
• On the basis of ultra structure and composition, 2 processes
are involved in development of enamel-
1. Organic matrix formation
2. Mineralization
ORGANIC MATRIX FORMATION
• Ameloblasts begin their secretory activity when small
amount of dentin has been laid down.
• The ameloblast loose their projection that has penetrated
basal lamina and islands of enamel matrix are deposited
along predentin.
• As enamel deposition proceeds a thin continuous layer of
enamel is formed along dentin. This is termed
dentinoenamel membrane.
89. Development of
TOME’S PROCESS
• As ameloblasts begin to
secrete enamel matrix,
they move away from
dentinal surface.
• So each cell forms a
conical projection. This
projection is called
Tomes’ proces.
• This gives junction
between enamel and
ameloblast a picket fence
or saw tooth appearance.
Pcw: Proximal cell web
Dcw: Distal cell web
90. •Enamel matrix formation as seen with the light
microscope. The Tomes’ processes of
ameloblasts jut into the matrix, creating a
picket-fence appearance.
91. •This tomes process contains primary secretory granules
and small vesicles whereas cell body cytoplasm contains
abundant synthetic organelles.
•Distinction between Tomes process and cell body is
clearly marked by distal terminal bars, which are localized
condensation of cytoplasmic substance associated with
thickened cell membranes.
92. • When the tomes process is
established, the secretion of the
enamel protein becomes
staggered and confined to two
sites..
1. Proximal parts of the process
close to junctional complexes
around periphery of cells, along
with adjoining ameloblasts,
which results in formation of
enamel matrix wall. These walls
enclosed a pit into which tomes
process fits.
2. Secretion from surface of
Tomes’ process, which fills the
pits, created previously.
93. •This difference of secretion sites gives structure to
enamel with walls becoming interred enamel and rest is
enamel rod.
•Tomes process persists until final few increments of
enamel are formed and as a result this are structure less.
94. MINERALISATION & MATURATION OF
ENAMEL MATRIX
• Ameloblast covering maturing enamel: Ameloblasts are
involved in cyclic process that is organic material is
removed from enamel matrix and minerals are
introduced. This is reflected on the cell morphology,
with cells alternating between possessing
1. A ruffle border (associated with introduction of
inorganic material)
2. A smooth border (associated with removal of protein and
water)
95. Theories responsible for mineralisation of
enamel are:-
Booster theory
Seeding theory
Matrix vesicle theory
The process is based on 2 mechanisms:-
Booster mechanism
Seeding mechanism
96. Mineralization of enamel takes place in 2 stages:
I. Primary or partial mineralization
• In this stage immediate partial mineralization
occurs in the matrix segments and interprismatic
substance as they are laid down.
• It accounts for 25-30% of total.
II. Maturation
• It is characterized by gradual completion of
mineralization
97. • It starts from the height of the
crown and progresses cervical,
however at each level maturation
seems to begin at the dentinal end
of rods.
• Thus, there is an integration of 2
processes,
1. Each rod matures from the depth
to surface.
2. Sequence of maturing rods is from
the cusps or incisal edge toward
cervical line.
98. •The advancing front is 1st
parallel to DEJ and later to outer
enamel surface. following this
pattern, the incisal and occlusal
regions reach maturity ahead of
cervical regions.
•At ultra structural level,
maturation is characterized by
growth of crystals seen in
primary phase.
99. •Original ribbon shaped crystals increase in
thickness more rapidly than width. To permit
such an increase, most of the enamel protein
must be displaced and removed, a function that
is well accomplished by ameloblasts.
100.
101. • Most common age change
• Attrition or wear of occlusal and
proximal surfaces
• Evidenced by loss of vertical
dimension of crown and by
flattening of proximal contour.
102. Interference during E. matrix formation may
cause Enamel hypoplasia.
Interference during Enamel maturation may
cause Enamel hypocalcification.
Each condition may be caused by systemic,
local, or hereditary factors.
103. • At eruption
– Prominent
• With age
– Reduced
• Generalized loss of rod ends.
• Flattening of perikymata.
• The rate at which structures are lost depends upon..
– Location of surface of tooth
– Location of tooth in mouth
104. Teeth darken with age
Increase in organic content
Deepening of dentine colour
Decrease in permeability
F-
ions increase on the surface
Nitrogen increases with age
their resistant to decay may be increased.
Reduced permeability of older teeth to fluid
Enamel may become harder with age.
105. DEFECTS IN AMELOGENESIS
•HERIDITARY
Defects in the amelogenin gene X linked form of
Amelogenesis imperfectia.
• ENVIRONMENTAL
Nutritional deficiencies: Vitamin A,C,D.
Ca&PO4.
• INFECTIONS
Trauma.
Fluoride, strontium, cobalt, manganese.
Drugs (chemotherapeutics, tetracycline
Porphyria
Erythroblastosis fetalis.
106.
107.
108.
109. Grooves and fissures on the occlusal surfaces of
molars and premolars– “weak spot for the action of
caries as the maintenance is difficult.
Use of fissure sealants
110. Lamellae, Tufts and spindles also may facilitate spread of
caries
111. Striae of Retzius
Are areas of hypomineralizaton thus facilitate the lateral spread of
caries
112. Fluoridation decrease caries
Fluorapatite crystal formation
Defects in the amelogenin gene X linked form of
Amelogenesis imperfectia
114. ORAL ANATOMY, HISTOLOGY AND EMBRYOLOGY
3RD
ed BERKOVITZ
ORAL HISTOLOGY, DEVELOPMENT, STRUCTURE AND
FUNCTION
7TH
ed TENCATE. A. R.
ORBAN’S ORAL HISTOLOGY AND EMBROLOGY
13TH
ed. BHASKAR.S.N.
ESSENTIALS OF ORAL HISTOLOGY AND EMBRYOLOGY
3RD
ed JAMES.K.AVERY.
COLOUR ATLAS OF ORAL HISTOLOGY AND EMBRYOLOGY.
BERKOVITZ.
Notes de l'éditeur
Decrease in permeability because the crystals acquire ions and decrease the pore size
Crystals ┴ to surface more vulnerable
Retwntion is by mechanical interlocking