Mining the Antibodypedia

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Lars Juhl Jensen

Technologie Business

Western blot
flow cytometry
immunoflourescence
immunohistochemistry
immunoprecipitation
ELISA
immunocytochemistry
protein array
gel shift
chromatin immunoprecipitation
proximity ligation assay
blocking/neutralizing
functional assay
radioimmunoassay
immunoelectron microscopy

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Indirect flow cytometry, also known as fluorescence-activated cell sorting (FACS) is a specialised type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. It is a useful scientific instrument as it provides fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest. In contrast to direct flow cytometry, FACS requires two incubation steps. Firstly with the appropriate primary antibody, then with a fluorochrome-labelled secondary antibody.

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Current CV . My objective is to obtain a rewarding and challenging research scientist position where my background and experience will contribute to the success of a growing company or research center. Currently, I am a Senior Associate Scientist at Amgen Inc. and certified Molecular Biologist with the American Society of Clinical Pathology MB (ASCP). I have more than 10 years of experience in the biotechnology/ pharmaceutical industry. I am highly proficient in various lab techniques, technologies, and automation. I demonstrated consistent success in the execution of assay development and method validation activities supporting clinical stage programs within GCP and GLP regulated environments. I possess extensive experience in optimization and validation of drug potency assays (ELISA and cell based assays), protein purification and characterization, and DNA/RNA extraction and quantitation. I am a subject matter expertise in the areas of human and rodent cell lines propagation and tissue dis-aggregation. I have proven operational capabilities in the establishment of standard operating procedures to ensure our laboratory meets regulatory and business requirements. I am a self-motivated professional who works effectively as an individual contributor or within a team matrix. As a quick learner, I can efficiently deliver results, easily adapt to changing environment and provide fresh ideas. My strengths include statistical analysis/guidance, report writing, and communication. Thank you in advance for your consideration. Please feel free to call me at (805-990-6258), or by e-mail at (mahawally46@gmail.com) if you have questions or would like a list of references. Sincerely, Maha Rizk

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