Custom peptide synthesis services
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LifeTein® is the only peptide manufacturer to offer the PeptideSynTM platform of peptide synthesis services. PeptideSynTM technology is optimized to provide a continuous flow of synthesized peptides, reducing costs while maintaining quality. PeptideSynTM has been proven to enhance the efficiency of synthesis.
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Custom peptide synthesis services
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- 2. Outline Market & Technology Trends LifeTein Technology Portfolio LifeTein Services 2
- 3. Synthetic Therapeutic Peptides More than 60 synthetic therapeutic peptides under 50 amino acids in size have reached the pharmaceutical market as APIs or generics. 3
- 4. Chemical Optimization Drug Discovery Today Volume 15, Numbers 1/2 January 2010 Strategies for Lead Peptide Optimization 4
- 5. Technology Trends Microwave Synthesis Using Nitroalkanes as Acyl Anion Equivalents www.nature.com/doifinder/10.1038/nature09125 Green Chemistry for Synthesis 5
- 6. LifeTein Technology Portfolio PeptideSynTM Platform Provides high-yield peptide synthesis Combines SPPS and LPPS for the highest synthesis success rate possible Ensures shorter turnaround 6
- 7. LifeTein Technology Portfolio PeptideSynTM Ligation Synthesizes long peptides (up to 120 amino acids) Ensures high yield and short turnaround 7
- 8. LifeTein Technology Portfolio A: H-MEDSTYYKASKGC-NH2 B: H-MEDST(PTyr)YKASKGC-NH2 C: H-PKYLQNTLKLATGMRNVPEKQTT-OH D: H-MEDSTYYKASKGC-NH2 E: H-MEDST(PTyr)YKASKGC-NH2 F: H-PKYLQNTLKLATGMRNVPEKQTT-OH 8
- 9. LifeTein Services Custom Peptide Synthesis Service Custom Peptide Library Synthesis Service Custom Polyclonal Antibody Service Custom Monoclonal Antibody Service 9
- 10. LifeTein Services Advantages of PeptideSynTM Technology Increases yields and reduces costs Shortens production cycle Combines the advantages of both solid and solution phase methods Can scale up projects rapidly to over 100 kg Avoids solubility problems for long segments in solution phase 10
- 11. Peptide Synthesis Service Peptide Length 5-120 amino acids Peptide Scale From mg to kg Peptide Purity Crude, desalted, >75%, >85%, >90%, >95%, 98%, and >99% Peptide 2-5 phosphorylation sites, methylation, acetylation, amidation, MAPs(2, 4 or 8 chains), more… Modifications Peptide Labels FITC, Biotin, 5-FAM, Rhodamine, Dansyl, TAMRA, more… Carrier Proteins KLH, BSA, OVA Cyclic Peptides Amide cyclic peptide (head to tail, side chain), 1, 2, or 3 disulfide bond bridges Analysis HPLC, MS, Amino Acid Analysis (AAA), solubility testing, microbial analysis, more… Peptide Libraries Peptide sequence length of 5-20 amino acids, 48, 96 peptides Others Free aliquots, spacers and linkers (Ahx, mini-PEG), more… 11
- 12. Free Aliquot Service 12
- 13. Case Studies Cell Penetrating Peptides Name Origin Sequence Tat family Tat (48-60) HIV-1 protein GRKKRRQRRRPPQQ Oligoarginine Tat derivative Rn Penetralia family p-Antp Antermapedia homeodomain RQIKIWFQNRRMKWKK plsl Igl-1 homeodomain RVIRVWFQNKRCKDKK Chimeric CPPs GWTLNSAGYLLGKINLKALAA Transportan Galanin-mastoparan LAKKIL 13
- 14. Case Studies Cell Penetrating Peptides Attached to a CPP, therapeutic cargo is delivered to an intracellular target, thus overcoming the entry restrictions set by the plasma membrane. 14
- 15. Case Studies N-Terminal Acetylation and C-Terminal Amidation Both modifications are free of charge Chemically synthesized peptides have free amino and carboxyl termini. N-terminal acetylation and C-terminal amidation reduce the overall charge of the peptide. This can 15 increase the stability of the peptide and its resemblance to the native protein.
- 16. Peptide Antigen Design Continuous versus Discontinuous Epitopes? 16
- 17. Peptide Antigen Design Sequence Length 8-20 amino acids • >75% for antibody generation, Peptide Purity • >90% for phosphorylated peptide antibody (recommended) • Keep the hydrophobic amino acid content below 50% Peptide Solubility • At least one charged residue for every five amino acids • Cys and Met can undergo rapid oxidation Difficult Amino Acids • Avoid multiple Ser or Pro • Avoid multiple or adjacent β-sheet forming amino acids: Glu, Iso, Leu, Phe, Thr, Tyr, Val Carrier Proteins KLH, BSA, OVA Multiple Antigenic Peptides (MAPs) 17
- 18. Custom Antibody Service • Polyclonal Antibodies Peptide Antigen Antibody Services • Monoclonal Antibodies • Polyclonal Antibodies Phosphorylated-Peptide Antibody Services • Monoclonal Antibodies • Polyclonal Antibodies Methylated-Peptide Antibody Services • Monoclonal Antibodies AdjuBoosterTM Adjuvant Technology 18
- 19. More information, please visit www.lifetein.com 19
- 20. Contact Information LifeTein LLC 150 Maple Ave South Plainfield, NJ 07080 USA info@lifetein.com 1-732-444-3282 1-888-791-1618 (Toll Free) 1-888-791-1618 (Fax) Let us be a partner to your growth! 20