This file is the package insert for Clodrosome-Clodronate encapsulated liposomes manufactured by Encapsula NanoSciences in United States of America. Clodrosome is used for macrophage and monocyte depletion. The formulation is for research purposes ONLY and it is used in laboratory animal models.
1. Encapsula
Nano
S ciences Product Information
Version: 02/15/12
Clodrosome® Macrophage Depletion Kit
Refrigerate at 4°C - DO NOT FREEZE
Kit Contents:
1. Clodrosome® Liposomal Clodronate Suspension - 5 ml in glass vial
Contains: Clodronate ((dichloro-phosphono-methyl)phosphonate) Disodium Salt 5 mg/ml
L-α-Phosphatidylcholine 18.8 mg/ml
Cholesterol 4.2 mg/ml
Suspended in Phosphate Buffered Saline at pH 7.4
2. Encapsome® Control Liposome Suspension - 5 ml in glass vial
Contains: L-α-Phosphatidylcholine 18.8 mg/ml
Cholesterol 4.2 mg/ml
Suspended in Phosphate Buffered Saline at pH 7.4
See page 2 for Product Handling and Administration
Receipt and Storage: Brief Product Description:
Inspect package for signs of exposure to extreme Clodrosome is a multilamellar liposome suspension in
conditions (excess heat, excess moisture, freezing, etc.) which clodronate is encapsulated in the aqueous
and ensure that vials are intact. If packaging is seriously compartments of the liposomes. Encapsome is formu-
damaged or otherwise suspect, immediately report lated and prepared identically to Clodrosome except
damage to courier (usually Federal Express at that clodronate is not added to the liposomes. The
1.800.463.3339), then contact ENS to arrange a replace- liposomes are filtered through 2 μm polycarbonate
ment delivery. membranes to ensure that larger particles, which may be
toxic to animals, are removed from the suspension. Both
Clodrosome and Encapsome should always be stored at
are prepared and packaged under sterile conditions.
in the dark at 4°C, except when brought to room temp-
erature for brief periods prior to animal dosing. DO NOT When animals or cells are treated with Clodrosome,
FREEZE. If the suspension is frozen, clodronate can be phagocytic cells recognize the liposomes as invading
released from the liposomes thus limiting its effective- foreign particles and proceed to remove the liposomes
ness in depleting macrophages. ENS is not responsible from the local tissue or serum via phagocytosis. The
for results generated by frozen product. liposomes then release clodronate into the cytosol
resulting in cell death. Unencapsulated clodronate
For General Information: cannot cross the cell membrane to initiate cell death.
We invite and encourage Clodrosome users and scien-
tists interested in Clodrosome to visit our website to Encapsome contol liposomes are recognized and
learn more about the behavior of Clodrosome as well as phagocytosed by the same mechanism as Clodrosome.
its numerous applications in research. All liposomes, Since the control liposomes do not contain clodronate,
including controls (no active agent), solicit some level of the phagocytic cells are not killed. However, phagocytes
immune system response when administered to animals. do respond to the ingestion of control liposomes by
Always take this into account when planning experi- cytokine secretion, temporary suspension of phagocytic
ments and interpreting the results. activity and other responses described in the literature.
Further details, including descriptive animations and a literature database, are available at
www.clodrosome.com
Version: 02/15/12 Page 1 of 2