Recombination DNA Technology (Nucleic Acid Hybridization )
enzymes.pptx
1. PATNA UNIVERSITY
DEPARTMENT OF BOTANY
A SEMINAR PRESENTATION ON
“ CLASSIFICATION OF ENZYMES”
SUBMITTED TO: SUBMITTED BY:
Dr. MEENAKSHI SINGH ALKA KUMARI
HOD, DEPARTMENT OF BOTANY ROLL NO.- 04
PATNA UNIVERSITY M.Sc. BOTANY
SEMESTER –II
SESSION: 2020-22
2. ENZYMES
Enzymes are proteins that functions as
biological catalysts. A catalyst is a substance
that speeds up a chemical reaction but isn’t
changed by the reaction.
Almost all enzymes are proteins and essential
to life.
In enzymatic reactions, the molecules at the
beginning of the process are called substrates,
and the enzyme converts them into different
molecules, the products.
3. Classification of Enzymes
Based on catalyzed reactions, the nomenclature
committee of the International Union of
Biochemistry and Molecular Biology(IUBMB)
recommended the following classifications:
1. OXIDOREDUCTASES
Catalyze a variety of oxidation-reduction
reactions.
Act on many chemical groups to add or remove
hydrogen atoms.
The common name will be dehydrogenase,
reductase.
4. 2. TRANSFERASES
Transfers chemical groups(acetyl, methyl,
phosphate etc.) from one molecule to another
or to another part of the same molecules.
Kinases are specialized transferases that
regulate metabolism by transferring phosphate
from ATP to other molecules.
5. 3. HYDROLASES
Catalyzes hydrolysis reactions where a
molecule is split into two or more smaller
molecules by the addition of water.
PROTEASES split protein molecules
HIV Protease is essential for HIV replication
Caspase plays a major role in apoptosis
NUCLEASES split nucleic acids(DNA and RNA)
Based on the substrate type , they are divided
into RNase and DNase.
6. 4. LYASES
Catalyzes the cleavage of C-C, C-O, C-S and C-
N bonds by means other than hydrolysis or
oxidation.
Common names include decarboxylase and
aldolase.
5. ISOMERASES
These enzymes catalyze geometric or structural
changes within one molecule.
According to type of isomerism, they may me
called cis-trans isomerases, tautomerases etc.
7. 6. LIGASES
Catalyze reactions in which two chemical groups
are joined(or ligated) with the use of energy from
ATP.
Examples include peptide synthase, DNA ligase
and RNA ligase.