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Maryam Shakeel
 The cell wall is the tough, usually flexible
but sometimes fairly rigid layer that
surrounds becteria. It is located outside
the cell membrane
 Mostly in bacteria cell wall chemically
composed of peptidoglycan (murein). The
cell wall strengthens the plasma
membrane and prevents the cell from
bursting.
Gram +ve      Gram-ve
with the       with the
Gram stain.    Gram
G+ cells are   stain. G-
Purple         cells are red.
Gram
                          Gram - E. coli cells
+ Staphylococcus cells.
   Gram-positive cell walls consist of many
    layers of peptidoglycan and also contain
    teichoic acids. Teichoic acids may:
   bind and regulate movement of cations into
    and out of the cell
   prevent extensive wall breakdown and
    possible cell lysis during cell growth
   provide much of the cell wall's antigenicity
   Gram-negative bacteria have a
    lipopolysaccharide-lipoprotein-phospholipid
    outer membrane surrounding a thin
    (sometimes a single) peptidoglycan layer.
    Gram-negative cell walls have no teichoic
    acids.
G +ve   G -ve
• From                                         • Maintain the
  Osmolysis                                      shape of cell




                                  Structural
                     Protection
                                   Support




                       Critical    Filtering
                      Structure   mechanism

• Becteria can                                 • Does not allow
  not live without                               motion of
  it                                             large molecules
   Surrounds the cell's cytoplasm and
    regulates the flow of substances in and out
    of the cell.
   The plasma membrane is composed of a
    phospholipid bilayer (two layers) embedded
    with proteins and other molecules. The
    phospholipids are not fixed relative to each
    other and able to flow past each
    other, making the membrane fluid.
    Bacteria can have a wide variety of fatty
    acids within their membranes. Along with
    typical saturated and unsaturated fatty
    acids, bacteria can contain fatty acids with
    additional methyl , hydroxy or even cyclic
    groups. The relative proportions of these
    fatty acids can be modulated by the
    bacterium to maintain the optimum fluidity of
    the membrane (e.g. following temperature
    change).
   Phospholipids are molecules composed of
    two long, hydrocarbon "tails" and a
    phosphate group "head." The hydrophilic
    (water-attracted) phosphate group "heads"
    form the internal and external surface of the
    plasma membrane. The hydrophobic (water-
    repelled) tails form the interior of the plasma
    membrane.
   Embedded in the plasma membrane are
    proteins that perform multiple functions,
    including the detection of chemicals in the
    cell's environment and the transportation of
    materials into and out of the cell.
   the region between the cytoplasmic and outer
    membranes. The periplasm contains the
    peptidoglycan layer and many proteins
    responsible for substrate binding on
    hydrolysis and reception of extracellular
    signals.
    passive transport of many ions, sugar and
    amino acids across the outer membrane.
    These molecules are therefore present in
    the periplasm.
   acting as a permeability barrier for most
    molecules and serving as the location for the
    transport of molecules into the cell.
   prokaryotic membranes also function in
    energy conservation as the location about
    which a proton motive force is generated.
   bacterial membranes (with some exceptions
    e.g. Mycoplasma and methanotrophs)
    generally do not contain sterols.
   bacteria can have a wide variety of fatty
    acids within their membranes.
   It prevents only       prevents toxicity to
    large molecules         the cell whereas cell
    from entering the       membrane prevents
    cell.                   entry of smaller
   Cell wall is            molecules.
    completely             cell membrane is
    permeable               semi-permeable.
   The function of the       The cell membrane
    cell wall is to            provides support to
    provide strength           the cytoskeleton of
    and rigidity to the        the cell, gives shape
    cell.                      to the cell
   It protects the cell      it maintains the
    against mechanical         potential of the cell,
    forces.                    helps in
   It is made up of           communication with
    peptidoglycan.             other cells, and act
                               as molecular signals
                              It is made up of lipid
                               and protein.

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Becterial

  • 2.  The cell wall is the tough, usually flexible but sometimes fairly rigid layer that surrounds becteria. It is located outside the cell membrane  Mostly in bacteria cell wall chemically composed of peptidoglycan (murein). The cell wall strengthens the plasma membrane and prevents the cell from bursting.
  • 3. Gram +ve Gram-ve with the with the Gram stain. Gram G+ cells are stain. G- Purple cells are red.
  • 4. Gram Gram - E. coli cells + Staphylococcus cells.
  • 5. Gram-positive cell walls consist of many layers of peptidoglycan and also contain teichoic acids. Teichoic acids may:  bind and regulate movement of cations into and out of the cell  prevent extensive wall breakdown and possible cell lysis during cell growth  provide much of the cell wall's antigenicity
  • 6.
  • 7. Gram-negative bacteria have a lipopolysaccharide-lipoprotein-phospholipid outer membrane surrounding a thin (sometimes a single) peptidoglycan layer. Gram-negative cell walls have no teichoic acids.
  • 8.
  • 9. G +ve G -ve
  • 10.
  • 11. • From • Maintain the Osmolysis shape of cell Structural Protection Support Critical Filtering Structure mechanism • Becteria can • Does not allow not live without motion of it large molecules
  • 12.
  • 13. Surrounds the cell's cytoplasm and regulates the flow of substances in and out of the cell.  The plasma membrane is composed of a phospholipid bilayer (two layers) embedded with proteins and other molecules. The phospholipids are not fixed relative to each other and able to flow past each other, making the membrane fluid.
  • 14. Bacteria can have a wide variety of fatty acids within their membranes. Along with typical saturated and unsaturated fatty acids, bacteria can contain fatty acids with additional methyl , hydroxy or even cyclic groups. The relative proportions of these fatty acids can be modulated by the bacterium to maintain the optimum fluidity of the membrane (e.g. following temperature change).
  • 15. Phospholipids are molecules composed of two long, hydrocarbon "tails" and a phosphate group "head." The hydrophilic (water-attracted) phosphate group "heads" form the internal and external surface of the plasma membrane. The hydrophobic (water- repelled) tails form the interior of the plasma membrane.
  • 16. Embedded in the plasma membrane are proteins that perform multiple functions, including the detection of chemicals in the cell's environment and the transportation of materials into and out of the cell.
  • 17. the region between the cytoplasmic and outer membranes. The periplasm contains the peptidoglycan layer and many proteins responsible for substrate binding on hydrolysis and reception of extracellular signals.
  • 18. passive transport of many ions, sugar and amino acids across the outer membrane. These molecules are therefore present in the periplasm.  acting as a permeability barrier for most molecules and serving as the location for the transport of molecules into the cell.
  • 19. prokaryotic membranes also function in energy conservation as the location about which a proton motive force is generated.  bacterial membranes (with some exceptions e.g. Mycoplasma and methanotrophs) generally do not contain sterols.  bacteria can have a wide variety of fatty acids within their membranes.
  • 20. It prevents only  prevents toxicity to large molecules the cell whereas cell from entering the membrane prevents cell. entry of smaller  Cell wall is molecules. completely  cell membrane is permeable semi-permeable.
  • 21. The function of the  The cell membrane cell wall is to provides support to provide strength the cytoskeleton of and rigidity to the the cell, gives shape cell. to the cell  It protects the cell  it maintains the against mechanical potential of the cell, forces. helps in  It is made up of communication with peptidoglycan. other cells, and act as molecular signals  It is made up of lipid and protein.

Notes de l'éditeur

  1.  Electron micrograph of an ultra-thin section of a dividing pair of group A streptococci (20,000X). The cell surface fimbriae (fibrils) are evident. The bacterial cell wall is seen as the light staining region between the fibrils and the dark staining cell interior. Cell division in progress is indicated by the new septum formed between the two cells and by the indentation of the cell wall near the cell equator. The streptococcal cell diameter is equal to approximately one micron. Electron micrograph of Streptococcus pyogenes by Maria Fazio and Vincent A. Fischetti, Ph.D. with permission. The Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University.For g+