The document discusses different types of cell cultures, including primary cultures derived from animal tissue, continuous cultures comprised of cell lines, and normal diploid cells with a finite lifespan. It describes common cell lines used in research, including HeLa cells from cervical carcinoma and CHO cells from hamster ovary. The key components of maintaining cell cultures are discussed, such as temperature, atmosphere, sterile conditions, culture medium, and growth equipment. Tasks demonstrate observing cell morphology, quantifying cell density using a hemocytometer, and counting chromosomes in CHO cells, which are shown to have an aneuploid number due to long-term cultivation.

1. Practical 4 In vitro cell cultivation

2. Tissue cultures Cell cultures In vitro cell cultivation

7. The biohazard box

8. Growth curve of the cell culture log N time lag log stationary phase decrease

10. Presentation Cell cultures X laboratory animals

11. Tasks

15. Task 3: Cell quantification using hemocytometer See microscopes on the table number 6.

18. Results

19. Task 2: Observation of HeLa cells Mitotic cells have circular shape.

20. Task 4: CHO cells 21 chromosomes Aneuploid number of chromosomes is a consequence of cell transformation during the long time cultivation. 7 6 4 4 5 6 3 7

21. Karyotype of CHO cells 21 chromosomes CHO cells have some features of tumor ones.

22. Karyotype of normal and tumor cell normal male cell tumor cell Rapid and uncontrolled proliferation of tumor cells leads to accumulation of numerical and structural chromosomal abnormalities.

25. See you next week! CHO cells