This document discusses Layar Merah Herbs Whitening Cream. It provides information on: 1) How the cream works to lighten skin tone and reduce spots through dual action preventing melanin synthesis and treating skin tone evenly without surgery or cosmetics. 2) In vitro and in vivo testing showing the cream significantly reduces melanin, tyrosinase activity, and skin darkening while lightening skin tone up to 28%. 3) Directions for use which include cleansing skin, applying twice daily, and taking a skin allergy test first due to potential reactions to preservatives.

1. Layar Merah Herbs

2. • Concern on the importance of
skin tone
• A fair and spotless skin, is
considered a sign of beauty
and reveals certain social status.
• A skin with a dull tone, spots
and irregular pigmentation
looks much older than the real
age
• Differences in skin color is due
to melanin
• Increase in melanin by external
aggression and aging leads to
changes in skin tone

3. • Surgery is costly, risky and
expensive
• Surgery is aggressive and
invasive
• Cosmetics is Natural, Safe and
Effective
• Whitening Cream decreases
skin tone evenly and
uniformly and reduces
different types of spots

4. • Dual action
• Prevents – Reduces melanin synthesis
• Treats – Evens out skin tone, reduces spots in
number and intensity
• Reduces process of spontaneous melanin
synthesis
• Prevents an accumulation of melanin in the skin
by reducing melanocyte dendrites
• Reduces tyrosinase enzyme formation by
63%, therefore it reduces melanogenesis
• Decreases the amount of pigment synthesized
by melanocytes by 34%, without affecting
their viability
• Modulates melanocyte gene expression
• Resulting in whitening of skin

5. • Instant skin tone whitening effect
• Long-term brightening effect
• Instant radiance boosting and moisturizing
• Decreases skin tone evenly and uniformly and reduces different
types of spots
• Lightening active ingredient in Whitening Cream diminishes
melanogenic activity and decreases skin tone and spots
• Tissue enlightment
• Preventing or inhibiting skin pigmentation
• Whitening Cream has an intelligent depigmentant; its activity is
higher in areas with spots and thus evens skin color

6. • Reduces spots in number and
intensity, regardless of their
origin
• Reduces both tyrosinase
enzyme formation and activity,
as well as melanin synthesis,
all without affecting
melanocyte viability.
• Decreases skin tone in a
uniform and homogenous way
• It reduces the number and
intensity of different types of
hyperpigmentation spots.

7. • Several test were performed to assess the in vitro
efficacy
• PPARγ binding affinity
• Study on depigmentation activity in human melanocytes :
• Evaluation of tyrosinase expression
• Evaluation of tyrosinase activity
• Evaluation of melanin quantity
• Incubation of NHEM cells (Normal Human Epidermal
Melanocytes) was conducted at a concentration of
0.7% for 6 and 9 days.

8. • The active ingredients of Whitening
Cream shows a good affinity to bind
to PPARγ and act as a natural
agonist
• It reduces tyrosinase enzyme
formation by 63%, therefore it
reduces melanogenesis
• It reduces tyrosinase activity by 55%
• It decreases the amount of pigment
synthesized by melanocytes by 34%,
without affecting their viability
• It prevents an accumulation of
melanin in the skin by reducing
melanocyte dendrites

9. • In vitro evaluation of melanin diminution
• Application of 4 mg/cm² of whitening creams on the surface
of reconstructed skin day 0, 1, 5, 6, 7, 8
• Spectrophotometric measurement of the rate of melanin
compared to a control after 9 days
• In vitro evaluation of skin lightening : ∆E
• Epidermis color measurement was performed before starting
treatment (Day 0) and 24h after the last application of
Whitening Cream on tissues containing melanocytes and
keratinocytes from African-american donors (spontaneus
production of melanin)
• Application of 4 mg/cm² of whitening creams on the surface
of reconstructed skin day 0, 1, 5, 6, 7, 8

10. • Chromatometric measure of the degree of pigmentation of
tissues. Quantification of the color of tissues using a Chroma
Minolta CR-400. Recording parameters L * (lightness), a *
and b * (chromaticity coordinates) Colour difference ∆E
• In vitro evaluation of skin lightening : ∆ ITA°
• Same than for ΔE with a skin spontaneus production of
melanin using a Chroma Minolta CR-400 and calculation of Δ
ITA°. ITA angle defines the degree of skin pigmentation
darker skin have lower ITA° values

11. • In vitro evaluation of melanin
diminution shows it has a
significative whitening activity of
around 25%. Melanin diminution is
linked to the inhibilition of
tyrasinase
• In vitro evaluation of skin
lightening : ∆E, shows a higher ∆E
shows a higher darkening ; it has a
significant whitening activity
• After 9 days of treatment the ITA°
values for tissues treated are
higher than untreated tissues
indicating a lighter tissue
coloration compared to untreated
tissues. It reduce the process of
spontaneous melanin synthesis
during the test

12. • In vivo efficacy
• 20 Asian volunteers
• 3% cosmetic formulation with active ingredient on one side of
the face, and placebo on the other.
• 56 days of twice daily application.
• Two areas of the same volunteer: Normal skin and
spotted skin

13. • Normal skin :
• It decreases the intensity of skin
pigmentation
• The ITA° parameter increases up to
28% in skin without spots, with a mean
variation of 3%
• Skin with spots :
• It lightens hyperpigmented areas
getting a smooth and uniform
complexion
• Whitening Cream has an intelligent
depigmentant; its activity is higher in
areas with spots and thus evens skin
color
• It reduces spots in number and
intensity, regardless of their origin

14. • The depigmenting potential of the active ingredient of the
Whitening Cream was assessed using human tanned in
vitro reconstructed epidermis. This in vitro experimental
study was based on the measurement of melanin content
and on the coloration of tissues after 9 days topical
treatment. The results show that it imparts :
• Significant melanin reduction
• Tissue enlightment
• This corroborates the down-regulating effect on melanin
level. These good results of melanin contents diminution
and lighter coloration after treatment prove the whitening
and depigmenting properties of the active ingredient

15. • Objectivation : Illuminating
• It contains mica to boost radiance of the skin for a radian, healthy looking
appearance
• Objectivation : Moisturizing
• It has moisturizing properties thanks to the polymeric coating
• Cosmetic benefits
• Instant whitening effect with a natural complexion – Lighten skin tone or
provide an even skin complexion
• Instant illuminating effect and moisturizing – A radiance-boosting
complexion perfector that smoothes and evens the skin for a luminous
glow and radiant, healthy-looking appearance
• Long-term whitening effect with a surface treatment based on an
efficient whitening active – Preventing or inhibiting skin pigmentation

16. • Skin whitening facial treatments
• Specific anti-spot facial treatments
• Skin lightening body lotions
• Anti-spot hand treatments
• Throat and neckline treatments
• Photo-aging treatments
• Anti-age treatments

17. • Discontinue use of other products 3 days prior to beginning
use of Whitening Cream
• Cleanse skin thoroughly
• Apply Whitening Cream to skin twice daily, gently
massage until all traces of cream disappear into the skin
• For best results, apply twice daily until desired results are
achieved
• Apply in the morning
• Allergic reactions are NOT COMMON and only happen to
a select group of people with very sensitive skin
• People with sensitive skin may react to the preservative
ingredients in the cream

18. • You should take a SKIN ALLERGY TEST with the cream
before applying these products to your face and skin
• The best way to prevent an allergic reaction is to know
if one will occur
• Prior to using the cream, apply a thin layer to a tiny
spot on the face that can be covered by your hair
• Throughout the day, observe it to see if the spot
becomes puffy, itchy or warm
• If all three occur, then you have an allergic reaction to
the cream and should not use it at all

19. • Titanium dioxide
• Aluminum hydroxide
• Glycyrrhiza glabra (Licorice) Root Extract
• Alpha-arbutin
• Boerhavia diffusa Root Extract