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© 2010 Pearson Education, Inc.
THE BASICS OF PHOTOSYNTHESIS
• Photosynthesis:
– Is used by plants, some protists, and some bacteria
– Transforms light energy into chemical energy
– Uses carbon dioxide and water as starting materials
• The chemical energy produced via photosynthesis is stored in the
bonds of sugar molecules.
© 2010 Pearson Education, Inc.
Chloroplasts: Sites of Photosynthesis
• Chloroplasts are:
– The site of photosynthesis
– Found mostly in the interior cells of leaves
© 2010 Pearson Education, Inc.
• Inside chloroplasts are membranous sacs called
thylakoids, which are suspended in a thick fluid, called stroma.
• Thylakoids are concentrated in stacks called grana.
• The green color of chloroplasts is from chlorophyll, a light-
absorbing pigment.
© 2010 Pearson Education, Inc.
• Stomata are tiny pores in leaves where carbon dioxide enters and
oxygen exits.
Leaf cross section
Stomata
Vein
CO2 O2
Figure 7.2-1
Leaf cross section
Stomata
Vein
CO2 O2
Interior cell LM
StromaGranum Thylakoid
Chloroplast
Outer
membrane
Inner
membrane
TEM
Figure 7.2-2
Leaf cross section
Stomata
Vein
CO2 O2
Figure 7.2a
StromaGranum Thylakoid
Chloroplast Outer
membrane
Inner
membrane
TEM
Figure 7.2b
© 2010 Pearson Education, Inc.
The Overall Equation for Photosynthesis
• In the overall equation for photosynthesis, notice that:
– The reactants of photosynthesis are the waste products of cellular
respiration.
Carbon
dioxide
6 O26 CO2
6 H2O C6H12O6
Water Glucose
Photo-
synthesis Oxygen gas
Light
energy
Figure 7.UN1
© 2010 Pearson Education, Inc.
• In photosynthesis:
– Sunlight provides the energy
– Electrons are boosted “uphill” and added to carbon dioxide
– Sugar is produced
© 2010 Pearson Education, Inc.
• During photosynthesis, water is split into:
– Hydrogen
– Oxygen
• Hydrogen is transferred along with electrons and added to carbon
dioxide to produce sugar.
• Oxygen escapes through stomata into the atmosphere.
© 2010 Pearson Education, Inc.
A Photosynthesis Road Map
• Photosynthesis occurs in two stages:
– The light reactions convert solar energy to chemical energy
– The Calvin cycle uses the products of the light reactions to make sugar
from carbon dioxide
Blast Animation: Photosynthesis: Light-Independent Reaction
Light
H2O
O2
Chloroplast
Light
reactions
NADPH
ATP
Figure 7.3-1
Light
H2O
O2
Chloroplast
Light
reactions
NADPH
ATP
Calvin
cycle
CO2
NADP+
ADP
P
Sugar
(C6H12O6)
Figure 7.3-2
© 2010 Pearson Education, Inc.
How Photosystems Harvest Light Energy
• Light behaves as photons, discrete packets of energy.
© 2010 Pearson Education, Inc.
• Chlorophyll molecules absorb photons.
– Electrons in the pigment gain energy.
– As the electrons fall back to their ground state, energy is released as heat
or light.
Light
Photon
Heat
Light (fluorescence)
Ground state
Chlorophyll
molecule
Excited state
e–
(a) Absorption of a photon
Figure 7.8a
© 2010 Pearson Education, Inc.
• A photosystem is a group of chlorophyll and other molecules that
function as a light-gathering antenna.
Chloroplast
Figure 7.9-1
Chloroplast
Thylakoid
membrane
Pigment molecules
Figure 7.9-2
Chloroplast
Thylakoid
membrane
Pigment molecules
Antenna pigment
molecules
Primary
electron
acceptor
Reaction-
center
chlorophyll a
Photon
Electron
transfer
Reaction
center
Photosystem
Transfer of energy
Figure 7.9-3
© 2010 Pearson Education, Inc.
How the Light Reactions Generate ATP and
NADPH
• Two types of photosystems cooperate in the light reactions:
– The water-splitting photosystem
– The NADPH-producing photosystem
Primary
electron
acceptor
Water-splitting
photosystem
Light
H2O
2 H
Reaction-
center
chlorophyll
2e–
2e–
O2++ 1
2
Figure 7.10-1
Primary
electron
acceptor
Water-splitting
photosystem
Light
H2O
2 H
Reaction-
center
chlorophyll
2e–
2e–
O2++
Energy
to make
ATP
1
2
Figure 7.10-2
Primary
electron
acceptor
Water-splitting
photosystem
Light
H2O
2 H
Reaction-
center
chlorophyll
2e–
2e–
O2++
Energy
to make
ATP
Primary
electron
acceptor
2e–
Light
NADPH-producing
photosystem
Reaction-
center
chlorophyll
2e–
NADPH
NADP
1
2
Figure 7.10-3
© 2010 Pearson Education, Inc.
• The light reactions are located in the thylakoid membrane.
© 2010 Pearson Education, Inc.
• An electron transport chain:
– Connects the two photosystems
– Releases energy that the chloroplast uses to make ATP
Light
H2O
Thylakoid
membrane
2e–
O2
ATP
NADP
Light
Stroma
Inside thylakoid
Photosystem Photosystem
Electron transport chain
NADPH
ADP P
H+
ATP
synthase
To Calvin cycle
H
Electron flow
H+
H
H
H
H
1
2
Figure 7.11
Light
H2O
Thylakoid
membrane
2e–
O2
ATP
NADP
Light
Stroma
Inside thylakoid
Photosystem Photosystem
Electron transport chain
NADPH
ADP P
ATP
synthase
To Calvin cycle
Electron flow
H
1
2
H
H
H
H H
H
Figure 7.11a
Water-splitting
photosystem
e–
ATP
NADPH
e–
e–
e–
e–
e–
e–
NADPH-producing
photosystem
Figure 7.12
THE CALVIN CYCLE: MAKING SUGAR
FROM CARBON DIOXIDE
• The Calvin cycle:
– Functions like a sugar factory within the stroma of a chloroplast
– Regenerates the starting material with each turn
© 2010 Pearson Education, Inc.
Calvin
cycle
P
P
P
Three-carbon moleculeRuBP sugar
CO2 (from air)
Figure 7.13-1
ATP
NADPH
Calvin
cycle
ADP  P
NADP
P
P
P
P
G3P sugar
Three-carbon moleculeRuBP sugar
CO2 (from air)
Figure 7.13-2
ATP
NADPH
ADP  P
NADP
P
P
P
P
P
P
G3P sugar
Three-carbon molecule
G3P sugar
G3P sugar
RuBP sugar
CO2 (from air)
Calvin
cycle
Glucose (and
other organic
compounds)
Figure 7.13-3
ATP
NADPH
Calvin
cycle
ADP  P
NADP
P
P
P
P
P
P
ADP  P
ATP
G3P sugar
Three-carbon molecule
G3P sugar
G3P sugar
RuBP sugar
CO2 (from air)
Glucose (and
other organic
compounds)
Figure 7.13-4
Evolution Connection:
Solar-Driven Evolution
• C3 plants:
– Use CO2 directly from the air
– Are very common and widely distributed
© 2010 Pearson Education, Inc.
© 2010 Pearson Education, Inc.
• C4 plants:
– Close their stomata to save water during hot and dry weather
– Can still carry out photosynthesis
• CAM plants:
– Are adapted to very dry climates
– Open their stomata only at night to conserve water
Sugar
C4 Pathway
(example: sugarcane)
C4 plant
Calvin
cycle
Cell
type 1
Four-carbon
compound
Cell
type 2
CO2
CO2
Figure 7.14a
CAM plant
Sugar
Calvin
cycle
Day
Four-carbon
compound
Night
CAM Pathway
(example: pineapple)
CO2
CO2
Figure 7.14b
Carbon
dioxide
6 O26 CO2
6 H2O C6H12O6
Water Glucose
Photo-
synthesis Oxygen gas
Light
energy
Figure 7.UN1
Light
reactions
CO2
O2
H2O
(C6H12O6)
NADPH
Light
Sugar
ATP
ADP
P
NADP
Calvin
cycle
Figure 7.UN2
Light
reactions
CO2
O2
H2O
(C6H12O6)
NADPH
Light
Sugar
ATP
ADP
P
NADP
Calvin
cycle
Figure 7.UN3
Carbon
dioxide
6 O26 CO2
6 H2O C6H12O6
Water Glucose
Photosynthesis
Oxygen
gas
Light energy
Figure 7.UN4
Light H2O
O2
Chloroplast
Light
reactions
NADPH
ATP
Calvin
cycle
CO2
NADP+
ADP
P
Sugar
(C6H12O6)
Stack of
thylakoids Stroma
Figure 7.UN5
acceptor
Water-splitting
photosystem
Photon
H2O
2 H
Chlorophyll
2e–
O2++
ATP
NADPH-producing
photosystem
Chlorophyll
NADPH
NADP+
e–
2e–
2e–
2e–
e–
acceptorADP
Photon
2
1
Figure 7.UN6
NADPH
Calvin
cycle
ADP P
NADP
P
ATP
G3P
CO2
Glucose and
other compounds
Figure 7.UN7

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