This document contains questions about protein function and enzyme kinetics calculations. It asks the reader to calculate a dissociation rate constant from given association rate and dissociation constant values. It also asks the reader to calculate the antigen concentration needed for 20% binding based on a given dissociation constant. Regarding enzyme kinetics, it asks the reader to calculate the Michaelis constant using the maximum reaction rate, enzyme concentration, substrate concentration, and known catalytic rate constant.