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Bacteriophage Reproduction Lytic and Lysogenic Cycles The following information is taken from: http://student.ccbcmd.edu/courses/bio141/lecguide/unit3/index.html#charvir
Bacteriophage Structure ,[object Object],[object Object],[object Object]
Bacteriophage Life Cycle Overview Lytic = steps 1, 2, 5, 6 Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Lysogenic = steps 1, 2, 3, 4 (5 & 6 occur later)
The Lytic Cycle The following information is taken from: http://student.ccbcmd.edu/courses/bio141/lecguide/unit3/index.html#charvir
Adsorption of a Bacteriophage to the Cell Wall of the Bacterium Protein on viral surface attaches to a specific receptor on the host cell surface.
Penetration of the Viral Genome into the Cytoplasm of the Bacterium This step is also known as ‘Entry’. The viral genome (DNA or RNA) enters the host cell.
Viral Replication and Maturation Viral DNA is transcribed and then translated by the host cell. The protein parts produced are then assembled together while still inside the host cell.
Release of the Bacteriophages by Lysis of the Bacterium The fully formed viruses burst from the host cell by lysing it.
http://www.youtube.com/watch?v=wVkCyU5aeeU&NR=1 ,[object Object]
Lytic Cycle Summary ,[object Object],[object Object],[object Object],[object Object],[object Object]
The Lysogenic Cycle The following information is taken from: http://student.ccbcmd.edu/courses/bio141/lecguide/unit3/index.html#charvir
Adsorption of a Bacteriophage to the Cell Wall of the Bacterium
Penetration of the Viral Genome into the Cytoplasm of the Bacterium
Formation of a Prophage The viral DNA is inserted into the host cell’s chromosome.  As the host cell replicates it’s own DNA and divides itself during mitosis, the provirus/prophage is replicated along with it.  The provirus/prophage lives dormant inside the host cell until... Provirus = viral DNA integrated into host DNA Prophage = bacteriophage viral DNA integrated into host DNA
Spontaneous Induction The separation of the provirus from the host chromosome.  At this point the virus will complete the steps of the lytic cycle.
Viral Replication and Maturation
Release of the Bacteriophages by Lysis of the Bacterium
Summary of the Entire Lysogenic Cycle ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
Comparing the two Reproductive Strategies Lytic versus Lysogenic
Lytic Cycle Lysogenic Cycle
 
Advantages and Limits ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Central Dogma DNA    RNA    Protein ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The viral genome directs the host cell's metabolic machinery (ribosomes, tRNA, nutrients, energy, enzymes, etc.) to synthesize viral enzymes and viral parts.
DNA viruses ,[object Object],[object Object],[object Object],[object Object],[object Object],Viral DNA can be integrated into the host cell genome so that it can then be transcribed and translated using the host cell’s machinery
RNA viruses ,[object Object],[object Object],[object Object],[object Object],[object Object],Viral RNA can be immediately translated with host cell’s machinery, skipping transcription.  This occurs in the cell’s cytoplasm.
Retroviruses (RNA Virus) ,[object Object],[object Object],[object Object],[object Object],Retroviruses have an enzyme that is capable of reverse transcribing their RNA into DNA.  This viral DNA can then be integrated into the host cell’s genome. Retroviruses replicate with a high mutation rate.  This increases it’s ability to evolve and survive.

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07 lytic vs lysogenic cycle

  • 1. Bacteriophage Reproduction Lytic and Lysogenic Cycles The following information is taken from: http://student.ccbcmd.edu/courses/bio141/lecguide/unit3/index.html#charvir
  • 2.
  • 3. Bacteriophage Life Cycle Overview Lytic = steps 1, 2, 5, 6 Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Lysogenic = steps 1, 2, 3, 4 (5 & 6 occur later)
  • 4. The Lytic Cycle The following information is taken from: http://student.ccbcmd.edu/courses/bio141/lecguide/unit3/index.html#charvir
  • 5. Adsorption of a Bacteriophage to the Cell Wall of the Bacterium Protein on viral surface attaches to a specific receptor on the host cell surface.
  • 6. Penetration of the Viral Genome into the Cytoplasm of the Bacterium This step is also known as ‘Entry’. The viral genome (DNA or RNA) enters the host cell.
  • 7. Viral Replication and Maturation Viral DNA is transcribed and then translated by the host cell. The protein parts produced are then assembled together while still inside the host cell.
  • 8. Release of the Bacteriophages by Lysis of the Bacterium The fully formed viruses burst from the host cell by lysing it.
  • 9.
  • 10.
  • 11. The Lysogenic Cycle The following information is taken from: http://student.ccbcmd.edu/courses/bio141/lecguide/unit3/index.html#charvir
  • 12. Adsorption of a Bacteriophage to the Cell Wall of the Bacterium
  • 13. Penetration of the Viral Genome into the Cytoplasm of the Bacterium
  • 14. Formation of a Prophage The viral DNA is inserted into the host cell’s chromosome. As the host cell replicates it’s own DNA and divides itself during mitosis, the provirus/prophage is replicated along with it. The provirus/prophage lives dormant inside the host cell until... Provirus = viral DNA integrated into host DNA Prophage = bacteriophage viral DNA integrated into host DNA
  • 15. Spontaneous Induction The separation of the provirus from the host chromosome. At this point the virus will complete the steps of the lytic cycle.
  • 16. Viral Replication and Maturation
  • 17. Release of the Bacteriophages by Lysis of the Bacterium
  • 18.
  • 19.
  • 20. Comparing the two Reproductive Strategies Lytic versus Lysogenic
  • 22.  
  • 23.
  • 24.
  • 25. The viral genome directs the host cell's metabolic machinery (ribosomes, tRNA, nutrients, energy, enzymes, etc.) to synthesize viral enzymes and viral parts.
  • 26.
  • 27.
  • 28.